##################################
# NO CUT ENZYMES (  195 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsiYI       BslI        BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        Tsp45I      Tth111I     TthHB8I     Van91I      
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AcsI          319  AAATTC               [GA]^AATT[CT]
AluI          344  AGCT                 AG^CT
AlwNI         340  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          184  CCTCAGG              CC^T[ACGT]AGG
ApoI          319  AAATTC               [GA]^AATT[CT]
AspHI          37  GTGCTC               G[AT]GC[AT]^C
BbsI          388  GAAGAC               GAAGAC(2/6)
BbvI          342  GCAGC                GCAGC(8/12)
BfaI          313  CTAG                 C^TAG
BmyI           37  GTGCTC               G[AGT]GC[ACT]^C
BpuAI         388  GAAGAC               GAAGAC(2/6)
BsaJI          29  CCCTGG               C^C[ACGT][ACGT]GG
BsiHKAI        37  GTGCTC               G[AT]GC[AT]^C
BsmAI         304  GAGAC                GTCTC(1/5)
Bsp1286I       37  GTGCTC               G[AGT]GC[ACT]^C
BspHI         512  TCATGA               T^CATGA
BsrI          207  CCAGT                ACTGG(1/-1)
BstF5I        300  GGATG                GGATG(2/0)
BstYI         517  AGATCC               [GA]^GATC[CT]
Bsu36I        184  CCTCAGG              CC^T[ACGT]AGG
Cfr13I         27  GGCCC                G^G[ACGT]CC
EarI          268  CTCTTC               CTCTTC(1/4)
Eco81I        184  CCTCAGG              CC^T[ACGT]AGG
Fnu4HI        342  GCAGC                GC^[ACGT]GC
FokI          300  GGATG                GGATG(9/13)
HphI          449  TCACC                GGTGA(8/7)
ItaI          342  GCAGC                GC^[ACGT]GC
Ksp632I       268  CTCTTC               CTCTTC(1/4)
MaeI          313  CTAG                 C^TAG
MaeIII         98  GTAAC                ^GT[ACGT]AC
MflI          517  AGATCC               [GA]^GATC[CT]
MseI          553  TTAA                 T^TAA
MspA1I        343  CAGCTG               C[AC]G^C[GT]G
PvuII         343  CAGCTG               CAG^CTG
RcaI          512  TCATGA               T^CATGA
Sau96I         27  GGCCC                G^G[ACGT]CC
SfaNI         369  GATGC                GCATC(5/9)
SmlI          221  CTCAAG               C^T[CT][GA]AG
Tru9I         553  TTAA                 T^TAA
TseI          342  GCAGC                G^C[AT]GC
Tsp509I       320  AATT                 ^AATT
TspRI         327  CACTG                CA[GC]TG[ACGT][ACGT]^
XbaI          312  TCTAGA               T^CTAGA
XhoII         517  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

AlwI          249  GATCC                GGATC(4/5)
              518  GATCC               
BseRI         395  CTCCTC               GAGGAG(10/8)
              190  GAGGAG              
BssKI          30  CCTGG                ^CC[ACGT]GG
              354  CCTGG               
BstNI          30  CCTGG                CC^[AT]GG
              354  CCTGG               
EcoRII         30  CCTGG                ^CC[AT]GG
              354  CCTGG               
HaeIII          3  GGCC                 GG^CC
               27  GGCC                
MvaI           30  CCTGG                CC^[AT]GG
              354  CCTGG               
PleI          283  GACTC                GAGTC(4/5)
              412  GACTC               
ScrFI          30  CCTGG                CC^[ACGT]GG
              354  CCTGG               
TfiI          137  GAATC                G^A[AT]TC
              446  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

DdeI        DpnII       HinfI       MboI        MboII       
MnlI        NdeII       NlaIII      Sau3AI