Restriction map for
pFN21AE3050
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BpmI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
KspI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI StyI SwaI
TaqI Tsp45I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 319 AAATTT [GA]^AATT[CT]
AluI 43 AGCT AG^CT
AlwI 249 GATCC GGATC(4/5)
AlwNI 340 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 319 AAATTT [GA]^AATT[CT]
Asp700I 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 37 GTGCTC G[AT]GC[AT]^C
BbsI 216 GAAGAC GAAGAC(2/6)
BbvI 292 GCTGC GCAGC(8/12)
BglII 517 AGATCT A^GATCT
BlpI 39 GCTCAGC GC^T[ACGT]AGC
BmyI 37 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC
BpuAI 216 GAAGAC GAAGAC(2/6)
BseRI 190 GAGGAG GAGGAG(10/8)
BsiHKAI 37 GTGCTC G[AT]GC[AT]^C
BsiYI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 391 GAGAC GTCTC(1/5)
Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C
BspHI 512 TCATGA T^CATGA
BsrI 207 CCAGT ACTGG(1/-1)
BstYI 517 AGATCT [GA]^GATC[CT]
CelII 39 GCTCAGC GC^T[ACGT]AGC
DraII 106 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 26 TGGCCG [CT]^GGCC[GA]
EcoO109I 106 AGGGCCT [GA]G^G[ACGT]CC[CT]
Fnu4HI 292 GCTGC GC^[ACGT]GC
ItaI 292 GCTGC GC^[ACGT]GC
MflI 517 AGATCT [GA]^GATC[CT]
MseI 553 TTAA T^TAA
NspI 361 GCATGT [GA]CATG^[CT]
PflMI 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 512 TCATGA T^CATGA
SmlI 221 CTCAAG C^T[CT][GA]AG
SspI 549 AATATT AAT^ATT
Tru9I 553 TTAA T^TAA
TseI 292 GCTGC G^C[AT]GC
Tsp509I 320 AATT ^AATT
TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 517 AGATCT [GA]^GATC[CT]
XmnI 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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BfaI 64 CTAG C^TAG
313 CTAG
BstF5I 417 CATCC GGATG(2/0)
383 GGATG
Cfr13I 3 GGCCC G^G[ACGT]CC
107 GGGCC
DdeI 40 CTCAG C^T[ACGT]AG
80 CTCAG
EarI 268 CTCTTC CTCTTC(1/4)
388 GAAGAG
FokI 417 CATCC GGATG(9/13)
383 GGATG
Ksp632I 268 CTCTTC CTCTTC(1/4)
388 GAAGAG
MaeI 64 CTAG C^TAG
313 CTAG
Sau96I 3 GGCCC G^G[ACGT]CC
107 GGGCC
TfiI 137 GAATC G^A[AT]TC
446 GAATC
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
BsaJI BssKI BstNI DpnII EcoRII
HaeIII HinfI MboI MboII MnlI
MvaI NdeII NlaIII PleI Sau3AI
ScrFI