Restriction map for
pFN21AE2100
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BpmI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI KspI MaeII
MaeIII MamI MfeI MluI MroI
MslI MspA1I MspI MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NlaIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tsp45I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 319 AAATTT [GA]^AATT[CT]
AluI 43 AGCT AG^CT
AlwNI 340 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 319 AAATTT [GA]^AATT[CT]
Asp700I 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 37 GTGCTC G[AT]GC[AT]^C
AvaI 184 CCCGAG C^[CT]CG[GA]G
BalI 201 TGGCCA TGG^CCA
BbsI 216 GAAGAC GAAGAC(2/6)
BbvI 292 GCTGC GCAGC(8/12)
BfaI 313 CTAG C^TAG
BlpI 39 GCTCAGC GC^T[ACGT]AGC
BmyI 37 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC
BpuAI 216 GAAGAC GAAGAC(2/6)
BsiHKAI 37 GTGCTC G[AT]GC[AT]^C
BsmAI 391 GAGAC GTCTC(1/5)
BsoBI 184 CCCGAG C^[CT]CG[GA]G
Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C
BspHI 512 TCATGA T^CATGA
BsrI 207 CCAGT ACTGG(1/-1)
BstF5I 417 CATCC GGATG(2/0)
BstYI 517 AGATCC [GA]^GATC[CT]
CelII 39 GCTCAGC GC^T[ACGT]AGC
DraII 106 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 106 AGGGCCT [GA]G^G[ACGT]CC[CT]
Fnu4HI 292 GCTGC GC^[ACGT]GC
FokI 417 CATCC GGATG(9/13)
ItaI 292 GCTGC GC^[ACGT]GC
MaeI 313 CTAG C^TAG
MflI 517 AGATCC [GA]^GATC[CT]
MluNI 201 TGGCCA TGG^CCA
MscI 201 TGGCCA TGG^CCA
MseI 553 TTAA T^TAA
NspI 361 GCATGT [GA]CATG^[CT]
PflMI 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 512 TCATGA T^CATGA
SmlI 221 CTCAAG C^T[CT][GA]AG
Tru9I 553 TTAA T^TAA
TseI 292 GCTGC G^C[AT]GC
Tsp509I 320 AATT ^AATT
TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 53 CCATCTGTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 517 AGATCC [GA]^GATC[CT]
XmnI 432 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 10 enzymes) #
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AlwI 249 GATCC GGATC(4/5)
518 GATCC
BseRI 187 GAGGAG GAGGAG(10/8)
190 GAGGAG
BsiYI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
183 CCCCGAGGAGG
BslI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
183 CCCCGAGGAGG
Cfr13I 3 GGCCC G^G[ACGT]CC
107 GGGCC
DdeI 40 CTCAG C^T[ACGT]AG
80 CTCAG
EaeI 26 TGGCCG [CT]^GGCC[GA]
201 TGGCCA
EarI 268 CTCTTC CTCTTC(1/4)
388 GAAGAG
Ksp632I 268 CTCTTC CTCTTC(1/4)
388 GAAGAG
Sau96I 3 GGCCC G^G[ACGT]CC
107 GGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
BsaJI BssKI BstNI DpnII EcoRII
HaeIII HinfI MboI MboII MnlI
MvaI NdeII NlaIII PleI Sau3AI
ScrFI TfiI