Restriction map for
pFN21AE2324
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# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccIII AciI AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeII MfeI MflI MluI MroI
MslI MspA1I MunI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StuI StyI SwaI TspRI
Tth111I Van91I XbaI XcmI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 73 GTAGAC GT^[AC][GT]AC
AccII 7 CGCG CG^CG
AflIII 985 ACATGT A^C[GA][CT]GT
AlwNI 506 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 973 GAGCAC G[AT]GC[AT]^C
AvaI 68 CTCGAG C^[CT]CG[GA]G
AvaII 160 GGACC G^G[AT]CC
BalI 61 TGGCCA TGG^CCA
BclI 840 TGATCA T^GATCA
BfaI 344 CTAG C^TAG
BmyI 973 GAGCAC G[AGT]GC[ACT]^C
BpmI 20 CTGGAG CTGGAG(16/14)
BsaBI 253 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 23 GAGACC GGTCTC(1/5)
BseRI 478 GAGGAG GAGGAG(10/8)
BsiHKAI 973 GAGCAC G[AT]GC[AT]^C
BsoBI 68 CTCGAG C^[CT]CG[GA]G
Bsp1286I 973 GAGCAC G[AGT]GC[ACT]^C
BspHI 141 TCATGA T^CATGA
BspLU11I 985 ACATGT A^CATGT
BspMI 546 ACCTGC ACCTGC(4/8)
BsrDI 386 GCAATG GCAATG(2/0)
BssKI 19 CCTGG ^CC[ACGT]GG
BstNI 19 CCTGG CC^[AT]GG
BstUI 7 CGCG CG^CG
EaeI 61 TGGCCA [CT]^GGCC[GA]
EcoNI 532 CCTAAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 19 CCTGG ^CC[AT]GG
EcoT22I 308 ATGCAT ATGCA^T
FbaI 840 TGATCA T^GATCA
HapII 742 CCGG C^CGG
HpaII 742 CCGG C^CGG
HphI 850 GGTGA GGTGA(8/7)
MaeI 344 CTAG C^TAG
MamI 253 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 61 TGGCCA TGG^CCA
MscI 61 TGGCCA TGG^CCA
MspI 742 CCGG C^CGG
MvaI 19 CCTGG CC^[AT]GG
MvnI 7 CGCG CG^CG
NruI 6 TCGCGA TCG^CGA
NsiI 308 ATGCAT ATGCA^T
NspI 985 ACATGT [GA]CATG^[CT]
PaeR7I 68 CTCGAG C^TCGAG
PstI 548 CTGCAG CTGCA^G
RcaI 141 TCATGA T^CATGA
ScrFI 19 CCTGG CC^[ACGT]GG
SmlI 68 CTCGAG C^T[CT][GA]AG
SpeI 343 ACTAGT A^CTAGT
XhoI 68 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 271 AAATTT [GA]^AATT[CT]
786 AAATTT
ApoI 271 AAATTT [GA]^AATT[CT]
786 AAATTT
BsiYI 434 CCAGAAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
532 CCTAAAAGAGG
BslI 434 CCAGAAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
532 CCTAAAAGAGG
BsmAI 23 GAGAC GTCTC(1/5)
145 GAGAC
BstF5I 393 GGATG GGATG(2/0)
699 GGATG
Cfr13I 160 GGACC G^G[ACGT]CC
708 GGGCC
DpnII 841 GATC ^GATC
937 GATC
FokI 393 GGATG GGATG(9/13)
699 GGATG
HincII 281 GTCAAC GT[CT]^[GA]AC
409 GTTGAC
HindII 281 GTCAAC GT[CT]^[GA]AC
409 GTTGAC
MboI 841 GATC ^GATC
937 GATC
MseI 230 TTAA T^TAA
515 TTAA
NdeII 841 GATC ^GATC
937 GATC
PleI 3 GACTC GAGTC(4/5)
528 GAGTC
Sau3AI 841 GATC ^GATC
937 GATC
Sau96I 160 GGACC G^G[ACGT]CC
708 GGGCC
SfaNI 310 GCATC GCATC(5/9)
508 GCATC
TaqI 69 TCGA T^CGA
939 TCGA
Tru9I 230 TTAA T^TAA
515 TTAA
Tsp509I 272 AATT ^AATT
787 AATT
TthHB8I 69 TCGA T^CGA
939 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BbvI Cac8I DdeI EarI
Fnu4HI HaeIII HinfI ItaI Ksp632I
MaeIII MboII MnlI NlaIII SfcI
TfiI TseI Tsp45I