Restriction map for
pFN21AB9945
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccIII AciI AclI
AcyI AfaI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbvCI
BcgI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
Bsp1286I Bsp1407I BspDI BspEI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MaeII MfeI MluI MroI MspA1I
MunI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 7 CGCG CG^CG
AflIII 1274 ACATGT A^C[GA][CT]GT
AlwI 1441 GGATC GGATC(4/5)
AlwNI 506 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1272 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 68 CTCGAG C^[CT]CG[GA]G
AvaII 160 GGACC G^G[AT]CC
BalI 61 TGGCCA TGG^CCA
BbsI 1492 GAAGAC GAAGAC(2/6)
BciVI 1061 GGATAC GTATCC(6/5)
BpmI 20 CTGGAG CTGGAG(16/14)
BpuAI 1492 GAAGAC GAAGAC(2/6)
BsaI 23 GAGACC GGTCTC(1/5)
BsaJI 1127 CCTTGG C^C[ACGT][ACGT]GG
BseRI 478 GAGGAG GAGGAG(10/8)
BsoBI 68 CTCGAG C^[CT]CG[GA]G
BspHI 141 TCATGA T^CATGA
BspLU11I 1274 ACATGT A^CATGT
BspMI 546 ACCTGC ACCTGC(4/8)
BsrI 1538 CCAGT ACTGG(1/-1)
BssKI 19 CCTGG ^CC[ACGT]GG
BstNI 19 CCTGG CC^[AT]GG
BstUI 7 CGCG CG^CG
BstYI 1441 GGATCT [GA]^GATC[CT]
EaeI 61 TGGCCA [CT]^GGCC[GA]
EcoNI 532 CCTAAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 19 CCTGG ^CC[AT]GG
EcoT14I 1127 CCTTGG C^C[AT][AT]GG
HapII 742 CCGG C^CGG
HpaII 742 CCGG C^CGG
MflI 1441 GGATCT [GA]^GATC[CT]
MluNI 61 TGGCCA TGG^CCA
MscI 61 TGGCCA TGG^CCA
MspI 742 CCGG C^CGG
MvaI 19 CCTGG CC^[AT]GG
MvnI 7 CGCG CG^CG
NruI 6 TCGCGA TCG^CGA
PaeR7I 68 CTCGAG C^TCGAG
PstI 548 CTGCAG CTGCA^G
RcaI 141 TCATGA T^CATGA
ScrFI 19 CCTGG CC^[ACGT]GG
SpeI 343 ACTAGT A^CTAGT
StuI 1124 AGGCCT AGG^CCT
StyI 1127 CCTTGG C^C[AT][AT]GG
XhoI 68 CTCGAG C^TCGAG
XhoII 1441 GGATCT [GA]^GATC[CT]
XmnI 1272 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 73 GTAGAC GT^[AC][GT]AC
1245 GTCTAC
BclI 840 TGATCA T^GATCA
1304 TGATCA
BsaBI 253 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1456 GATGGTAATC
BsiYI 434 CCAGAAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
532 CCTAAAAGAGG
BslI 434 CCAGAAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
532 CCTAAAAGAGG
BsrDI 386 GCAATG GCAATG(2/0)
1487 GCAATG
EcoT22I 308 ATGCAT ATGCA^T
1116 ATGCAT
FbaI 840 TGATCA T^GATCA
1304 TGATCA
HincII 281 GTCAAC GT[CT]^[GA]AC
409 GTTGAC
HindII 281 GTCAAC GT[CT]^[GA]AC
409 GTTGAC
MamI 253 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1456 GATGGTAATC
MslI 1179 CACAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1308 CACCCAAGTG
NsiI 308 ATGCAT ATGCA^T
1116 ATGCAT
NspI 1065 ACATGC [GA]CATG^[CT]
1274 ACATGT
SfcI 548 CTGCAG C^T[GA][CT]AG
554 CTACAG
SmlI 68 CTCGAG C^T[CT][GA]AG
1451 CTCAAG
TspRI 1196 CACTG CA[GC]TG[ACGT][ACGT]^
1539 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AcsI AluI ApoI BbvI BfaI
BsmAI BstF5I Cac8I Cfr13I DdeI
DpnII EarI Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
NdeII NlaIII PleI Sau3AI Sau96I
SfaNI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TthHB8I