Restriction map for
pFN21AE1765
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MaeII MfeI MflI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AciI 141 CCGC CCGC(-3/-1)
AlwI 703 GATCC GGATC(4/5)
AvaII 300 GGTCC G^G[AT]CC
BanII 610 GAGCTC G[GA]GC[CT]^C
BbsI 442 GAAGAC GAAGAC(2/6)
BbvI 247 GCTGC GCAGC(8/12)
BclI 390 TGATCA T^GATCA
BfaI 314 CTAG C^TAG
BpmI 707 CTGGAG CTGGAG(16/14)
BpuAI 442 GAAGAC GAAGAC(2/6)
BsaBI 730 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 495 GTCTC GTCTC(1/5)
BsmI 691 GAATGC GAATGC(1/-1)
BspHI 324 TCATGA T^CATGA
BsrBI 141 CCGCTC CCGCTC(-3/-3)
BstEII 549 GGTAACC G^GT[ACGT]ACC
BstF5I 417 GGATG GGATG(2/0)
BstPI 549 GGTAACC G^GT[ACGT]ACC
Cfr13I 300 GGTCC G^G[ACGT]CC
DraIII 601 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 759 CTCTTC CTCTTC(1/4)
EcoO65I 549 GGTAACC G^GT[ACGT]ACC
FbaI 390 TGATCA T^GATCA
Fnu4HI 247 GCTGC GC^[ACGT]GC
FokI 417 GGATG GGATG(9/13)
HincII 254 GTCAAC GT[CT]^[GA]AC
HindII 254 GTCAAC GT[CT]^[GA]AC
ItaI 247 GCTGC GC^[ACGT]GC
Ksp632I 759 CTCTTC CTCTTC(1/4)
MaeI 314 CTAG C^TAG
MamI 730 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MwoI 61 GCAAGCAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 299 GGGTCC GG[ACGT]^[ACGT]CC
NspI 582 GCATGC [GA]CATG^[CT]
RcaI 324 TCATGA T^CATGA
SacI 610 GAGCTC GAGCT^C
Sau96I 300 GGTCC G^G[ACGT]CC
SphI 582 GCATGC GCATG^C
TseI 247 GCTGC G^C[AT]GC
Tsp45I 521 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 264 GTCTAC GT^[AC][GT]AC
754 GTAGAC
AspHI 317 GAGCAC G[AT]GC[AT]^C
610 GAGCTC
BmyI 317 GAGCAC G[AGT]GC[ACT]^C
610 GAGCTC
BsiHKAI 317 GAGCAC G[AT]GC[AT]^C
610 GAGCTC
BsiYI 96 CCAACAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
191 CCACCAAAAGG
BslI 96 CCAACAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
191 CCACCAAAAGG
Bsp1286I 317 GAGCAC G[AGT]GC[ACT]^C
610 GAGCTC
BssKI 642 CCAGG ^CC[ACGT]GG
706 CCTGG
BstNI 642 CCAGG CC^[AT]GG
706 CCTGG
Cac8I 61 GCAAGC GC[ACGT]^[ACGT]GC
582 GCATGC
DpnII 391 GATC ^GATC
703 GATC
EcoRII 642 CCAGG ^CC[AT]GG
706 CCTGG
HphI 520 GGTGA GGTGA(8/7)
349 TCACC
MboI 391 GATC ^GATC
703 GATC
MslI 616 CATAAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
686 CATTTGAATG
MvaI 642 CCAGG CC^[AT]GG
706 CCTGG
NdeII 391 GATC ^GATC
703 GATC
Sau3AI 391 GATC ^GATC
703 GATC
ScrFI 642 CCAGG CC^[ACGT]GG
706 CCTGG
TfiI 328 GATTC G^A[AT]TC
514 GAATC
TspRI 206 CACTG CA[GC]TG[ACGT][ACGT]^
632 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AcsI AfaI AluI ApoI DdeI
HaeIII HinfI MaeIII MboII MnlI
MseI NlaIII PleI RsaI StuI
Tru9I Tsp509I