Restriction map for
pFN21AE5329
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII BamHI
BanI BbeI BbsI BbvCI BcgI
BclI BcnI BfrI BglI BglII
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HapII HgaI
Hin1I HincII HindII HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MroI MseI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AocI 76 CCTAAGG CC^T[ACGT]AGG
AspHI 418 GAGCTC G[AT]GC[AT]^C
AvrII 243 CCTAGG C^CTAGG
BanII 418 GAGCTC G[GA]GC[CT]^C
BbrPI 592 CACGTG CAC^GTG
BciVI 457 GTATCC GTATCC(6/5)
BfaI 244 CTAG C^TAG
BlnI 243 CCTAGG C^CTAGG
BmyI 418 GAGCTC G[AGT]GC[ACT]^C
BsaAI 592 CACGTG [CT]AC^GT[GA]
BseRI 229 GAGGAG GAGGAG(10/8)
BsiHKAI 418 GAGCTC G[AT]GC[AT]^C
BsmI 121 GAATGC GAATGC(1/-1)
Bsp1286I 418 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 86 TGTACA T^GTACA
BspMI 424 ACCTGC ACCTGC(4/8)
BsrGI 86 TGTACA T^GTACA
BstEII 525 GGTCACC G^GT[ACGT]ACC
BstPI 525 GGTCACC G^GT[ACGT]ACC
BstXI 218 CCACTTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 76 CCTAAGG CC^T[ACGT]AGG
Cac8I 613 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 540 GCGC GCG^C
DpnII 3 GATC ^GATC
DraIII 203 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 76 CCTAAGG CC^T[ACGT]AGG
EcoO65I 525 GGTCACC G^GT[ACGT]ACC
EcoT22I 303 ATGCAT ATGCA^T
HhaI 540 GCGC GCG^C
HinP1I 540 GCGC G^CGC
HindIII 359 AAGCTT A^AGCTT
HinfI 345 GACTC G^A[ACGT]TC
MaeI 244 CTAG C^TAG
MboI 3 GATC ^GATC
NdeII 3 GATC ^GATC
NsiI 303 ATGCAT ATGCA^T
PflMI 550 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 345 GACTC GAGTC(4/5)
PmaCI 592 CACGTG CAC^GTG
PmlI 592 CACGTG CAC^GTG
SacI 418 GAGCTC GAGCT^C
Sau3AI 3 GATC ^GATC
SexAI 521 ACCAGGT A^CC[AT]GGT
SfaNI 689 GCATC GCATC(5/9)
SmlI 269 CTCAAG C^T[CT][GA]AG
SspBI 86 TGTACA T^GTACA
Tsp509I 32 AATT ^AATT
Van91I 550 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AvaII 503 GGACC G^G[AT]CC
647 GGACC
BalI 463 TGGCCA TGG^CCA
611 TGGCCA
BpmI 498 CTCCAG CTGGAG(16/14)
226 CTGGAG
BsrDI 693 CATTGC GCAATG(2/0)
107 GCAATG
Cfr13I 503 GGACC G^G[ACGT]CC
647 GGACC
DdeI 77 CTAAG C^T[ACGT]AG
727 CTAAG
DraII 502 AGGACCC [GA]G^G[ACGT]CC[CT]
646 AGGACCC
EaeI 463 TGGCCA [CT]^GGCC[GA]
611 TGGCCA
EcoNI 243 CCTAGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
516 CCTCTACCAGG
EcoO109I 502 AGGACCC [GA]G^G[ACGT]CC[CT]
646 AGGACCC
EcoT14I 153 CCTTGG C^C[AT][AT]GG
243 CCTAGG
MaeII 470 ACGT A^CGT
593 ACGT
MboII 5 TCTTC GAAGA(8/7)
757 TCTTC
MluNI 463 TGGCCA TGG^CCA
611 TGGCCA
MscI 463 TGGCCA TGG^CCA
611 TGGCCA
MspA1I 235 CAGCTG C[AC]G^C[GT]G
570 CAGCTG
NlaIII 100 CATG CATG^
145 CATG
PpuMI 502 AGGACCC [GA]G^G[AT]CC[CT]
646 AGGACCC
PvuII 235 CAGCTG CAG^CTG
570 CAGCTG
Sau96I 503 GGACC G^G[ACGT]CC
647 GGACC
SfcI 366 CTACAG C^T[GA][CT]AG
434 CTACAG
StuI 251 AGGCCT AGG^CCT
513 AGGCCT
StyI 153 CCTTGG C^C[AT][AT]GG
243 CCTAGG
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BsiYI
BslI BsmFI BsrI BssKI BstF5I
BstNI EcoRII Fnu4HI FokI HaeIII
HphI ItaI MaeIII MnlI MvaI
MwoI NlaIV RsaI ScrFI TseI
Tsp45I TspRI