Restriction map for
pFN21AE5332
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmFI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DsaI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AciI 336 CCGC CCGC(-3/-1)
AfaI 333 GTAC GT^AC
AflIII 460 ACATGT A^C[GA][CT]GT
AocI 646 CCTGAGG CC^T[ACGT]AGG
ApaI 251 GGGCCC GGGCC^C
Asp700I 564 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 432 GGATCC G^GATCC
BanI 358 GGTGCC G^G[CT][GA]CC
BanII 251 GGGCCC G[GA]GC[CT]^C
BbsI 214 GAAGAC GAAGAC(2/6)
BbvCI 469 GCTGAGG CCTCAGC(-5/-2)
BclI 344 TGATCA T^GATCA
BcnI 254 CCCGG CC^[GC]GG
BmyI 251 GGGCCC G[AGT]GC[ACT]^C
BpuAI 214 GAAGAC GAAGAC(2/6)
BsiYI 78 CCTATATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 78 CCTATATGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 865 GAGAC GTCTC(1/5)
BsmBI 865 GAGACG CGTCTC(1/5)
BsmI 115 GAATGC GAATGC(1/-1)
Bsp1286I 251 GGGCCC G[AGT]GC[ACT]^C
BspHI 26 TCATGA T^CATGA
BspLU11I 460 ACATGT A^CATGT
Bsu36I 646 CCTGAGG CC^T[ACGT]AGG
DrdI 511 GACCATCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 646 CCTGAGG CC^T[ACGT]AGG
FbaI 344 TGATCA T^GATCA
HapII 255 CCGG C^CGG
HindIII 565 AAGCTT A^AGCTT
HpaII 255 CCGG C^CGG
HphI 440 TCACC GGTGA(8/7)
MfeI 110 CAATTG C^AATTG
MseI 781 TTAA T^TAA
MslI 303 CACAGATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 255 CCGG C^CGG
MunI 110 CAATTG C^AATTG
MwoI 341 GCATGATCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 254 CCCGG CC^[GC]GG
NspI 460 ACATGT [GA]CATG^[CT]
PleI 714 GACTC GAGTC(4/5)
PpuMI 74 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 662 CAGCTG CAG^CTG
RcaI 26 TCATGA T^CATGA
RsaI 333 GTAC GT^AC
SfcI 240 CTACAG C^T[GA][CT]AG
TfiI 593 GAATC G^A[AT]TC
Tru9I 781 TTAA T^TAA
XmnI 564 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 121 AAATTC [GA]^AATT[CT]
419 GAATTT
ApoI 121 AAATTC [GA]^AATT[CT]
419 GAATTT
AvaII 75 GGACC G^G[AT]CC
267 GGACC
BseRI 559 GAGGAG GAGGAG(10/8)
862 GAGGAG
BstNI 147 CCTGG CC^[AT]GG
279 CCTGG
BstYI 432 GGATCC [GA]^GATC[CT]
642 AGATCC
DraII 74 AGGACCT [GA]G^G[ACGT]CC[CT]
250 AGGGCCC
EcoO109I 74 AGGACCT [GA]G^G[ACGT]CC[CT]
250 AGGGCCC
EcoRII 147 CCTGG ^CC[AT]GG
279 CCTGG
HaeIII 252 GGCC GG^CC
486 GGCC
HinfI 593 GAATC G^A[ACGT]TC
714 GACTC
MboII 214 GAAGA GAAGA(8/7)
773 TCTTC
MflI 432 GGATCC [GA]^GATC[CT]
642 AGATCC
MspA1I 336 CCGCTG C[AC]G^C[GT]G
662 CAGCTG
MvaI 147 CCTGG CC^[AT]GG
279 CCTGG
SfaNI 322 GATGC GCATC(5/9)
818 GCATC
XhoII 432 GGATCC [GA]^GATC[CT]
642 AGATCC
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsgI
BsrI BssKI BstF5I Cfr13I DdeI
DpnII EcoT14I Fnu4HI FokI ItaI
MaeIII MboI MnlI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI StyI
TseI Tsp45I Tsp509I TspRI