Restriction map for
pFN21AE5672
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AseI AsnI
Asp718I AspEI AspI BamHI BbvCI
BciVI BclI BfrI BglII BsaBI
BseAI BsgI BsiWI BsmFI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BstBI ClaI CpoI DraI
Eam1105I Eco47III EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HinfI HpaI KpnI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PleI
PmeI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SbfI ScaI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I XbaI XcmI XmaCI
XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AcyI 728 GGCGCC G[GA]^CG[CT]C
AocI 978 CCTGAGG CC^T[ACGT]AGG
ApaI 261 GGGCCC GGGCC^C
AscI 1170 GGCGCGCC GG^CGCGCC
Asp700I 753 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1145 TGCGCA TGC^GCA
AvrII 366 CCTAGG C^CTAGG
BanII 261 GGGCCC G[GA]GC[CT]^C
BbeI 728 GGCGCC GGCGC^C
BbrPI 910 CACGTG CAC^GTG
BcgI 599 GCATCTGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 772 GCCGCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 366 CCTAGG C^CTAGG
BsaAI 910 CACGTG [CT]AC^GT[GA]
BsaHI 728 GGCGCC G[GA]^CG[CT]C
BsaI 1396 GAGACC GGTCTC(1/5)
BsaWI 1183 ACCGGA [AT]^CCGG[AT]
BsrDI 1431 CATTGC GCAATG(2/0)
BsrFI 346 GCCGGC [GA]^CCGG[CT]
BssSI 652 CACGAG CACGAG(-5/-1)
Bst1107I 1521 GTATAC GTA^TAC
BstEII 240 GGTCACC G^GT[ACGT]ACC
BstPI 240 GGTCACC G^GT[ACGT]ACC
BstXI 782 CCATCGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 644 AGATCC [GA]^GATC[CT]
BstZ17I 1521 GTATAC GTA^TAC
Bsu36I 978 CCTGAGG CC^T[ACGT]AGG
Cfr10I 346 GCCGGC [GA]^CCGG[CT]
DraIII 1300 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 460 GACCCGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 464 CGGCCG C^GGCCG
EclXI 464 CGGCCG C^GGCCG
Eco52I 464 CGGCCG C^GGCCG
Eco81I 978 CCTGAGG CC^T[ACGT]AGG
EcoO65I 240 GGTCACC G^GT[ACGT]ACC
FauI 720 CCCGC CCCGC(4/6)
FspI 1145 TGCGCA TGC^GCA
Hin1I 728 GGCGCC G[GA]^CG[CT]C
KasI 728 GGCGCC G^GCGCC
KspI 1352 CCGCGG CCGC^GG
MaeIII 241 GTCAC ^GT[ACGT]AC
MflI 644 AGATCC [GA]^GATC[CT]
NaeI 346 GCCGGC GCC^GGC
NarI 728 GGCGCC GG^CGCC
NcoI 1256 CCATGG C^CATGG
NgoMIV 346 GCCGGC G^CCGGC
NspI 704 ACATGC [GA]CATG^[CT]
PmaCI 910 CACGTG CAC^GTG
PmlI 910 CACGTG CAC^GTG
SacII 1352 CCGCGG CCGC^GG
SapI 1245 GCTCTTC GCTCTTC(1/4)
SfiI 771 GGCCGCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 728 GGCGCC GGC^GCC
Tsp45I 241 GTCAC ^GT[GC]AC
Tsp509I 487 AATT ^AATT
XhoII 644 AGATCC [GA]^GATC[CT]
XmnI 753 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 645 GATCC GGATC(4/5)
1442 GGATC
AlwNI 39 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1045 CAGCTGCTG
BanI 100 GGTGCC G^G[CT][GA]CC
728 GGCGCC
BcnI 462 CCCGG CC^[GC]GG
500 CCGGG
BfaI 367 CTAG C^TAG
961 CTAG
BlpI 405 GCTAAGC GC^T[ACGT]AGC
627 GCTGAGC
Bpu1102I 405 GCTAAGC GC^T[ACGT]AGC
627 GCTGAGC
BsiEI 453 CGACCG CG[GA][CT]^CG
464 CGGCCG
BsmBI 813 CGTCTC CGTCTC(1/5)
21 GAGACG
BsrBI 1033 CCGCTC CCGCTC(-3/-3)
613 GAGCGG
BstAPI 651 GCACGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1299 GCACGATGTGC
CelII 405 GCTAAGC GC^T[ACGT]AGC
627 GCTGAGC
DpnII 645 GATC ^GATC
1443 GATC
HgaI 360 GACGC GACGC(5/10)
1130 GCGTC
MaeI 367 CTAG C^TAG
961 CTAG
MaeII 305 ACGT A^CGT
911 ACGT
MboI 645 GATC ^GATC
1443 GATC
NciI 462 CCCGG CC^[GC]GG
500 CCGGG
NdeII 645 GATC ^GATC
1443 GATC
PaeR7I 943 CTCGAG C^TCGAG
1054 CTCGAG
PflMI 1089 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1224 CCAGCAGGTGG
Sau3AI 645 GATC ^GATC
1443 GATC
SexAI 1007 ACCTGGT A^CC[AT]GGT
1016 ACCTGGT
SfcI 36 CTACAG C^T[GA][CT]AG
1497 CTACAG
TspRI 196 CAGTG CA[GC]TG[ACGT][ACGT]^
1216 CAGTG
Van91I 1089 CCAGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1224 CCAGCAGGTGG
XhoI 943 CTCGAG C^TCGAG
1054 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AspHI AvaI AvaII BalI BbsI
BbvI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BspMI BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NlaIII NlaIV PvuII RsaI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TseI TthHB8I