Restriction map for
pFN21AB7044
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII AccI AccIII AcsI AflII
AgeI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI AvrII
BalI BamHI BbrPI BcgI BclI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaI BsaWI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EarI Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI Ksp632I
MfeI MflI MluI MluNI MroI
MscI MslI MunI NdeI NheI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tsp509I Van91I XhoI XhoII
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 1100 GGTACC G^GTACC
AclI 1042 AACGTT AA^CGTT
AflIII 1013 ACACGT A^C[GA][CT]GT
AlwNI 899 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 847 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1100 GGTACC G^GTACC
AspI 304 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 207 TGCGCA TGC^GCA
BbeI 60 GGCGCC GGCGC^C
BciVI 610 GGATAC GTATCC(6/5)
BglI 373 GCCGAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1302 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 156 CTGCAC GTGCAG(16/14)
BsiEI 386 CGGCCG CG[GA][CT]^CG
BsmAI 476 GAGAC GTCTC(1/5)
BsmBI 476 GAGACG CGTCTC(1/5)
Bsp1407I 26 TGTACA T^GTACA
BsrBI 187 GAGCGG CCGCTC(-3/-3)
BsrFI 224 GCCGGC [GA]^CCGG[CT]
BsrGI 26 TGTACA T^GTACA
BstXI 1052 CCAGTCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 224 GCCGGC [GA]^CCGG[CT]
DraIII 1167 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 386 CGGCCG C^GGCCG
EclXI 386 CGGCCG C^GGCCG
Eco52I 386 CGGCCG C^GGCCG
EcoNI 690 CCTGATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 207 TGCGCA TGC^GCA
KasI 60 GGCGCC G^GCGCC
KpnI 1100 GGTACC GGTAC^C
KspI 383 CCGCGG CCGC^GG
MamI 1302 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 763 TTAA T^TAA
NaeI 224 GCCGGC GCC^GGC
NarI 60 GGCGCC GG^CGCC
NcoI 102 CCATGG C^CATGG
NgoMIV 224 GCCGGC G^CCGGC
NotI 385 GCGGCCGC GC^GGCCGC
PflFI 304 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1042 AACGTT AA^CGTT
PvuII 1204 CAGCTG CAG^CTG
SacII 383 CCGCGG CCGC^GG
SexAI 830 ACCTGGT A^CC[AT]GGT
SfoI 60 GGCGCC GGC^GCC
SmaI 421 CCCGGG CCC^GGG
SspBI 26 TGTACA T^GTACA
TfiI 681 GAATC G^A[AT]TC
Tru9I 763 TTAA T^TAA
Tth111I 304 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1393 TCTAGA T^CTAGA
XmaCI 421 CCCGGG C^CCGGG
XmaI 421 CCCGGG C^CCGGG
XmnI 847 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 27 GTAC GT^AC
1101 GTAC
AhdI 478 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1369 GACGAGAGGTC
AlwI 866 GATCC GGATC(4/5)
991 GGATC
ApaI 286 GGGCCC GGGCC^C
699 GGGCCC
AspEI 478 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1369 GACGAGAGGTC
AspHI 802 GAGCTC G[AT]GC[AT]^C
954 GAGCTC
AvaII 828 GGACC G^G[AT]CC
1211 GGTCC
BanI 60 GGCGCC G^G[CT][GA]CC
1100 GGTACC
BbvCI 1113 GCTGAGG CCTCAGC(-5/-2)
1206 GCTGAGG
BseRI 1383 CTCCTC GAGGAG(10/8)
531 GAGGAG
BsiHKAI 802 GAGCTC G[AT]GC[AT]^C
954 GAGCTC
BspMI 160 ACCTGC ACCTGC(4/8)
210 GCAGGT
DpnII 866 GATC ^GATC
992 GATC
EaeI 380 TGGCCG [CT]^GGCC[GA]
386 CGGCCG
Eam1105I 478 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1369 GACGAGAGGTC
EcoT14I 102 CCATGG C^C[AT][AT]GG
1058 CCTTGG
MaeII 1015 ACGT A^CGT
1043 ACGT
MboI 866 GATC ^GATC
992 GATC
NdeII 866 GATC ^GATC
992 GATC
PpuMI 827 GGGACCT [GA]G^G[AT]CC[CT]
1210 AGGTCCC
RsaI 27 GTAC GT^AC
1101 GTAC
SacI 802 GAGCTC GAGCT^C
954 GAGCTC
Sau3AI 866 GATC ^GATC
992 GATC
SfaNI 1110 GATGC GCATC(5/9)
173 GCATC
StyI 102 CCATGG C^C[AT][AT]GG
1058 CCTTGG
TspRI 510 CAGTG CA[GC]TG[ACGT][ACGT]^
522 CACTG
XcmI 236 CCAACGAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
977 CCAAATGGTCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
BanII BbsI BbvI BcnI BfaI
BmyI BpuAI BsaHI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssHII BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
PstI Sau96I ScrFI SfcI SmlI
TaqI TseI Tsp45I TthHB8I