Restriction map for
pFN21AE1178
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AocI Aor51HI AscI Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspI Tth111I Van91I XbaI
XcmI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Alw44I 31 GTGCAC G^TGCAC
AlwI 1271 GGATC GGATC(4/5)
ApaLI 31 GTGCAC G^TGCAC
AseI 649 ATTAAT AT^TAAT
AsnI 649 ATTAAT AT^TAAT
BalI 69 TGGCCA TGG^CCA
BanI 225 GGCACC G^G[CT][GA]CC
BcgI 33 GCACTTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiWI 746 CGTACG C^GTACG
BsmBI 368 GAGACG CGTCTC(1/5)
Bsp1407I 1336 TGTACA T^GTACA
BspMI 200 ACCTGC ACCTGC(4/8)
BsrDI 1991 GCAATG GCAATG(2/0)
BsrGI 1336 TGTACA T^GTACA
BstYI 1271 GGATCT [GA]^GATC[CT]
DraIII 840 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 69 TGGCCA [CT]^GGCC[GA]
EarI 181 GAAGAG CTCTTC(1/4)
EcoRV 628 GATATC GAT^ATC
EcoT22I 1147 ATGCAT ATGCA^T
HgaI 496 GACGC GACGC(5/10)
Ksp632I 181 GAAGAG CTCTTC(1/4)
MaeII 371 ACGT A^CGT
MflI 1271 GGATCT [GA]^GATC[CT]
MluNI 69 TGGCCA TGG^CCA
MscI 69 TGGCCA TGG^CCA
NsiI 1147 ATGCAT ATGCA^T
PleI 1284 GAGTC GAGTC(4/5)
PshAI 318 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 649 ATTAAT AT^TAAT
PstI 202 CTGCAG CTGCA^G
SapI 181 GAAGAGC GCTCTTC(1/4)
SbfI 201 CCTGCAGG CCTGCA^GG
SmaI 415 CCCGGG CCC^GGG
Sse8387I 201 CCTGCAGG CCTGCA^GG
SspBI 1336 TGTACA T^GTACA
SwaI 1404 ATTTAAAT ATTT^AAAT
TaqI 197 TCGA T^CGA
Tsp45I 427 GTGAC ^GT[GC]AC
TthHB8I 197 TCGA T^CGA
XhoII 1271 GGATCT [GA]^GATC[CT]
XmaCI 415 CCCGGG C^CCGGG
XmaI 415 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 747 GTAC GT^AC
1337 GTAC
AlwNI 55 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
777 CAGGCTCTG
ApaI 77 GGGCCC GGGCC^C
1814 GGGCCC
AvaI 41 CTCGGG C^[CT]CG[GA]G
415 CCCGGG
BbsI 1909 GAAGAC GAAGAC(2/6)
772 GTCTTC
BpuAI 1909 GAAGAC GAAGAC(2/6)
772 GTCTTC
BsgI 755 GTGCAG GTGCAG(16/14)
1746 GTGCAG
BsmAI 368 GAGAC GTCTC(1/5)
466 GAGAC
BsoBI 41 CTCGGG C^[CT]CG[GA]G
415 CCCGGG
DpnII 159 GATC ^GATC
1272 GATC
DraI 832 TTTAAA TTT^AAA
1405 TTTAAA
DsaI 187 CCATGG C^C[GA][CT]GG
732 CCATGG
EcoRI 1214 GAATTC G^AATTC
1961 GAATTC
HphI 1731 GGTGA GGTGA(8/7)
488 TCACC
MaeIII 10 GTAAC ^GT[ACGT]AC
427 GTGAC
MboI 159 GATC ^GATC
1272 GATC
NcoI 187 CCATGG C^CATGG
732 CCATGG
NdeII 159 GATC ^GATC
1272 GATC
PpuMI 191 GGGTCCT [GA]G^G[AT]CC[CT]
232 AGGACCT
PvuII 1042 CAGCTG CAG^CTG
1294 CAGCTG
RsaI 747 GTAC GT^AC
1337 GTAC
Sau3AI 159 GATC ^GATC
1272 GATC
SmlI 954 CTCAAG C^T[CT][GA]AG
2022 CTCAAG
StuI 275 AGGCCT AGG^CCT
1106 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AluI ApoI Asp700I
AspHI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DraII EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII ItaI
MaeI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NlaIII NlaIV SacI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp509I TspRI XmnI