Restriction map for
pFN21AE9520
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbeI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BseAI BsiHKAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI Eam1105I
Eco47III EcoRI EcoRV EcoT22I FbaI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 861 CGCG CG^CG
AcyI 917 GGCGTC G[GA]^CG[CT]C
AfaI 670 GTAC GT^AC
AflIII 1292 ACGTGT A^C[GA][CT]GT
AocI 796 CCTAAGG CC^T[ACGT]AGG
AvaI 508 CTCGAG C^[CT]CG[GA]G
BbrPI 1291 CACGTG CAC^GTG
BbsI 638 GTCTTC GAAGAC(2/6)
BcnI 243 CCGGG CC^[GC]GG
BfaI 487 CTAG C^TAG
BpuAI 638 GTCTTC GAAGAC(2/6)
BsaHI 917 GGCGTC G[GA]^CG[CT]C
BsaWI 24 TCCGGT [AT]^CCGG[AT]
BseRI 204 GAGGAG GAGGAG(10/8)
BsiEI 1204 CGGCCG CG[GA][CT]^CG
BsmAI 151 GTCTC GTCTC(1/5)
BsmBI 150 CGTCTC CGTCTC(1/5)
BsmFI 235 GTCCC GGGAC(10/14)
BsmI 327 GCATTC GAATGC(1/-1)
BsoBI 508 CTCGAG C^[CT]CG[GA]G
BsrBI 550 CCGCTC CCGCTC(-3/-3)
BsrDI 1211 CATTGC GCAATG(2/0)
BssSI 1120 CTCGTG CACGAG(-5/-1)
BstEII 192 GGTGACC G^GT[ACGT]ACC
BstPI 192 GGTGACC G^GT[ACGT]ACC
BstUI 861 CGCG CG^CG
BstYI 86 GGATCT [GA]^GATC[CT]
Bsu36I 796 CCTAAGG CC^T[ACGT]AGG
DraIII 298 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 226 GACGAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 983 CCATGG C^C[GA][CT]GG
EagI 1204 CGGCCG C^GGCCG
EclXI 1204 CGGCCG C^GGCCG
Eco52I 1204 CGGCCG C^GGCCG
Eco81I 796 CCTAAGG CC^T[ACGT]AGG
EcoNI 486 CCTAGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 192 GGTGACC G^GT[ACGT]ACC
FseI 1201 GGCCGGCC GGCCGG^CC
HgaI 918 GCGTC GACGC(5/10)
Hin1I 917 GGCGTC G[GA]^CG[CT]C
MaeI 487 CTAG C^TAG
MflI 86 GGATCT [GA]^GATC[CT]
MspA1I 620 CAGCTG C[AC]G^C[GT]G
MvnI 861 CGCG CG^CG
NciI 243 CCGGG CC^[GC]GG
NcoI 983 CCATGG C^CATGG
NspI 370 GCATGC [GA]CATG^[CT]
PaeR7I 508 CTCGAG C^TCGAG
PflMI 36 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1291 CACGTG CAC^GTG
PmlI 1291 CACGTG CAC^GTG
PvuII 620 CAGCTG CAG^CTG
RsaI 670 GTAC GT^AC
SfaNI 1283 GCATC GCATC(5/9)
SphI 370 GCATGC GCATG^C
Van91I 36 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 508 CTCGAG C^TCGAG
XhoII 86 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwI 86 GGATC GGATC(4/5)
688 GGATC
BalI 980 TGGCCA TGG^CCA
1229 TGGCCA
BpmI 697 CTCCAG CTGGAG(16/14)
201 CTGGAG
BsaAI 1162 TACGTG [CT]AC^GT[GA]
1291 CACGTG
BstF5I 240 CATCC GGATG(2/0)
1233 CATCC
DpnII 87 GATC ^GATC
689 GATC
DraII 233 AGGTCCC [GA]G^G[ACGT]CC[CT]
1000 AGGTCCT
EcoO109I 233 AGGTCCC [GA]G^G[ACGT]CC[CT]
1000 AGGTCCT
EcoT14I 983 CCATGG C^C[AT][AT]GG
1004 CCTTGG
FokI 240 CATCC GGATG(9/13)
1233 CATCC
HaeII 1062 GGCGCT [GA]GCGC^[CT]
1255 GGCGCT
HinfI 736 GAGTC G^A[ACGT]TC
1330 GACTC
HphI 192 GGTGA GGTGA(8/7)
742 TCACC
MboI 87 GATC ^GATC
689 GATC
MluNI 980 TGGCCA TGG^CCA
1229 TGGCCA
MscI 980 TGGCCA TGG^CCA
1229 TGGCCA
NdeII 87 GATC ^GATC
689 GATC
PleI 1330 GACTC GAGTC(4/5)
736 GAGTC
PpuMI 233 AGGTCCC [GA]G^G[AT]CC[CT]
1000 AGGTCCT
Sau3AI 87 GATC ^GATC
689 GATC
StyI 983 CCATGG C^C[AT][AT]GG
1004 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanI
BanII BbvI BglI BmyI BsaJI
BsgI BsiYI BslI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstNI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI EaeI EarI EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI Ksp632I MaeII MaeIII
MboII MnlI MslI MspI MvaI
MwoI NaeI NgoMIV NlaIII NlaIV
Sau96I ScrFI SmlI StuI TaqI
TseI Tsp45I TspRI TthHB8I