Restriction map for
pFN21AE2785
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# NO CUT ENZYMES ( 166 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI TfiI TseI Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 385 GACGTC GACGT^C
AccII 69 CGCG CG^CG
AlwI 281 GGATC GGATC(4/5)
AlwNI 546 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 172 GAGCAC G[AT]GC[AT]^C
AvaII 321 GGTCC G^G[AT]CC
AviII 351 TGCGCA TGC^GCA
BanII 145 GAGCCC G[GA]GC[CT]^C
BpmI 329 CTGGAG CTGGAG(16/14)
BsiHKAI 172 GAGCAC G[AT]GC[AT]^C
BsmAI 554 GTCTC GTCTC(1/5)
BsmFI 95 GGGAC GGGAC(10/14)
BsmI 514 GAATGC GAATGC(1/-1)
BsrI 328 ACTGG ACTGG(1/-1)
BssHII 68 GCGCGC G^CGCGC
BstF5I 558 CATCC GGATG(2/0)
BstNI 128 CCAGG CC^[AT]GG
BstUI 69 CGCG CG^CG
BstYI 281 GGATCT [GA]^GATC[CT]
DpnII 282 GATC ^GATC
DraI 202 TTTAAA TTT^AAA
EcoRII 128 CCAGG ^CC[AT]GG
EcoT14I 40 CCAAGG C^C[AT][AT]GG
Fnu4HI 461 GCGGC GC^[ACGT]GC
FokI 558 CATCC GGATG(9/13)
FspI 351 TGCGCA TGC^GCA
HphI 389 TCACC GGTGA(8/7)
ItaI 461 GCGGC GC^[ACGT]GC
MaeIII 388 GTCAC ^GT[ACGT]AC
MboI 282 GATC ^GATC
MflI 281 GGATCT [GA]^GATC[CT]
MvaI 128 CCAGG CC^[AT]GG
MvnI 69 CGCG CG^CG
NdeII 282 GATC ^GATC
PstI 11 CTGCAG CTGCA^G
Sau3AI 282 GATC ^GATC
SbfI 10 CCTGCAGG CCTGCA^GG
SfaNI 184 GATGC GCATC(5/9)
Sse8387I 10 CCTGCAGG CCTGCA^GG
StuI 365 AGGCCT AGG^CCT
StyI 40 CCAAGG C^C[AT][AT]GG
TaqI 191 TCGA T^CGA
Tsp45I 388 GTCAC ^GT[GC]AC
TthHB8I 191 TCGA T^CGA
XhoII 281 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 30 GACGCC G[GA]^CG[CT]C
385 GACGTC
AluI 379 AGCT AG^CT
547 AGCT
BcnI 92 CCGGG CC^[GC]GG
497 CCGGG
BsaHI 30 GACGCC G[GA]^CG[CT]C
385 GACGTC
BseRI 46 GAGGAG GAGGAG(10/8)
79 GAGGAG
BsgI 58 GTGCAG GTGCAG(16/14)
264 GTGCAG
BsiYI 39 CCCAAGGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
312 CCATCAGAGGG
BslI 39 CCCAAGGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
312 CCATCAGAGGG
Cfr13I 116 GGCCC G^G[ACGT]CC
321 GGTCC
EarI 63 GAAGAG CTCTTC(1/4)
169 GAAGAG
HaeIII 116 GGCC GG^CC
366 GGCC
Hin1I 30 GACGCC G[GA]^CG[CT]C
385 GACGTC
HinfI 156 GAGTC G^A[ACGT]TC
405 GACTC
Ksp632I 63 GAAGAG CTCTTC(1/4)
169 GAAGAG
NciI 92 CCGGG CC^[GC]GG
497 CCGGG
PleI 405 GACTC GAGTC(4/5)
156 GAGTC
SapI 63 GAAGAGC GCTCTTC(1/4)
169 GAAGAGC
Sau96I 116 GGCCC G^G[ACGT]CC
321 GGTCC
SfcI 11 CTGCAG C^T[GA][CT]AG
543 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI Asp700I BbsI BmyI BpuAI
BsaJI Bsp1286I BssKI Cac8I CfoI
DdeI FauI HapII HgaI HhaI
HinP1I HpaII MaeII MboII MnlI
MseI MspI MwoI NlaIV ScrFI
Tru9I TspRI XmnI