Restriction map for
pFN21AE4269
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI AspEI AspI AvaI
AviII BbrPI BbvCI BcgI BclI
BfrI BglI BsiEI BsiWI BsoBI
BspDI BspHI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI FbaI
FseI FspI KspI MfeI MluI
MslI MunI NdeI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 986 GGTACC G^GTACC
AccIII 1013 TCCGGA T^CCGGA
AcsI 1563 AAATTT [GA]^AATT[CT]
AflIII 2097 ACACGT A^C[GA][CT]GT
AlwNI 596 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2192 AGCGCT AGC^GCT
ApaI 2542 GGGCCC GGGCC^C
ApoI 1563 AAATTT [GA]^AATT[CT]
Asp700I 2755 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 986 GGTACC G^GTACC
AvrII 2383 CCTAGG C^CTAGG
BamHI 1010 GGATCC G^GATCC
BbeI 632 GGCGCC GGCGC^C
BciVI 931 GGATAC GTATCC(6/5)
BlnI 2383 CCTAGG C^CTAGG
BlpI 3031 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3031 GCTCAGC GC^T[ACGT]AGC
BsaAI 2104 CACGTA [CT]AC^GT[GA]
BsaBI 2152 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1013 TCCGGA [AT]^CCGG[AT]
BseAI 1013 TCCGGA T^CCGGA
BsmI 1469 GCATTC GAATGC(1/-1)
BspEI 1013 TCCGGA T^CCGGA
BsrFI 920 GCCGGC [GA]^CCGG[CT]
BstAPI 2653 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2828 CCACAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 3031 GCTCAGC GC^T[ACGT]AGC
Cfr10I 920 GCCGGC [GA]^CCGG[CT]
DraIII 421 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2192 AGCGCT AGC^GCT
EcoNI 2869 CCTAAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 940 ATGCAT ATGCA^T
HindIII 1960 AAGCTT A^AGCTT
HpaI 1996 GTTAAC GTT^AAC
KasI 632 GGCGCC G^GCGCC
KpnI 986 GGTACC GGTAC^C
MamI 2152 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1013 TCCGGA T^CCGGA
NaeI 920 GCCGGC GCC^GGC
NarI 632 GGCGCC GG^CGCC
NcoI 1259 CCATGG C^CATGG
NgoMIV 920 GCCGGC G^CCGGC
NheI 2994 GCTAGC G^CTAGC
NsiI 940 ATGCAT ATGCA^T
PflMI 2800 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 3253 GAGCTC GAGCT^C
SfoI 632 GGCGCC GGC^GCC
SphI 938 GCATGC GCATG^C
StuI 2476 AGGCCT AGG^CCT
Van91I 2800 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 189 TCTAGA T^CTAGA
XmnI 2755 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 143 CGCG CG^CG
348 CGCG
BalI 1517 TGGCCA TGG^CCA
3318 TGGCCA
BanII 2542 GGGCCC G[GA]GC[CT]^C
3253 GAGCTC
BcnI 2393 CCGGG CC^[GC]GG
2494 CCCGG
BsmBI 3098 GAGACG CGTCTC(1/5)
3157 GAGACG
BstUI 143 CGCG CG^CG
348 CGCG
EaeI 1517 TGGCCA [CT]^GGCC[GA]
3318 TGGCCA
EcoRV 1161 GATATC GAT^ATC
1221 GATATC
FauI 2748 CCCGC CCCGC(4/6)
1285 GCGGG
HaeII 632 GGCGCC [GA]GCGC^[CT]
2192 AGCGCT
HgaI 3100 GACGC GACGC(5/10)
3159 GACGC
HincII 1858 GTTGAC GT[CT]^[GA]AC
1996 GTTAAC
HindII 1858 GTTGAC GT[CT]^[GA]AC
1996 GTTAAC
MluNI 1517 TGGCCA TGG^CCA
3318 TGGCCA
MscI 1517 TGGCCA TGG^CCA
3318 TGGCCA
MvnI 143 CGCG CG^CG
348 CGCG
NciI 2393 CCGGG CC^[GC]GG
2494 CCCGG
NspI 938 GCATGC [GA]CATG^[CT]
1071 ACATGC
PpuMI 2906 GGGACCT [GA]G^G[AT]CC[CT]
2960 AGGACCT
SmlI 484 CTCAAG C^T[CT][GA]AG
1702 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaII BanI BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII