Restriction map for
pFN21AE2377
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BcgI BcnI BlnI BsaHI
BseAI BsiWI BsmBI BsmFI BspDI
BspEI BspMI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraII DraIII
Eam1105I Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FseI FspI
HaeII Hin1I HindIII HpaI KasI
KpnI MfeI MluI MroI MunI
NarI NciI NcoI NdeI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RsrII SacI SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 584 GTAGAC GT^[AC][GT]AC
AflII 2976 CTTAAG C^TTAAG
AlwNI 2095 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 2014 ATTAAT AT^TAAT
AsnI 2014 ATTAAT AT^TAAT
AvaI 3890 CTCGAG C^[CT]CG[GA]G
BalI 326 TGGCCA TGG^CCA
BanII 57 GGGCTC G[GA]GC[CT]^C
BciVI 1897 GGATAC GTATCC(6/5)
BclI 1551 TGATCA T^GATCA
BfrI 2976 CTTAAG C^TTAAG
BglI 124 GCCGGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2824 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2824 GCTAAGC GC^T[ACGT]AGC
BsaI 3640 GAGACC GGTCTC(1/5)
BsmI 3573 GAATGC GAATGC(1/-1)
BsoBI 3890 CTCGAG C^[CT]CG[GA]G
BspLU11I 2521 ACATGT A^CATGT
BsrBI 117 CCGCTC CCGCTC(-3/-3)
BsrFI 124 GCCGGC [GA]^CCGG[CT]
BstAPI 1225 GCAAGCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1850 CCAAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2824 GCTAAGC GC^T[ACGT]AGC
Cfr10I 124 GCCGGC [GA]^CCGG[CT]
DraI 1363 TTTAAA TTT^AAA
DrdI 1016 GACCAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 62 CCTTGG C^C[AT][AT]GG
FbaI 1551 TGATCA T^GATCA
MluNI 326 TGGCCA TGG^CCA
MscI 326 TGGCCA TGG^CCA
NaeI 124 GCCGGC GCC^GGC
NgoMIV 124 GCCGGC G^CCGGC
PaeR7I 3890 CTCGAG C^TCGAG
PshBI 2014 ATTAAT AT^TAAT
PvuII 2432 CAGCTG CAG^CTG
SpeI 1981 ACTAGT A^CTAGT
SphI 1056 GCATGC GCATG^C
StyI 62 CCTTGG C^C[AT][AT]GG
XbaI 3319 TCTAGA T^CTAGA
XcmI 53 CCACGGGCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3890 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Asp700I 3304 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3719 GAAATAATTC
BbrPI 212 CACGTG CAC^GTG
2587 CACGTG
BglII 223 AGATCT A^GATCT
1970 AGATCT
BsaAI 212 CACGTG [CT]AC^GT[GA]
2587 CACGTG
BseRI 1092 CTCCTC GAGGAG(10/8)
3342 CTCCTC
BsgI 28 CTGCAC GTGCAG(16/14)
1449 GTGCAG
BsiEI 102 CGGCCG CG[GA][CT]^CG
122 CGGCCG
BsmAI 3640 GAGAC GTCTC(1/5)
3794 GAGAC
Bsp1407I 653 TGTACA T^GTACA
3781 TGTACA
BspHI 1481 TCATGA T^CATGA
3987 TCATGA
BsrGI 653 TGTACA T^GTACA
3781 TGTACA
EagI 102 CGGCCG C^GGCCG
122 CGGCCG
EclXI 102 CGGCCG C^GGCCG
122 CGGCCG
Eco52I 102 CGGCCG C^GGCCG
122 CGGCCG
FauI 145 CCCGC CCCGC(4/6)
166 GCGGG
HgaI 1958 GACGC GACGC(5/10)
462 GCGTC
HincII 2137 GTTGAC GT[CT]^[GA]AC
3037 GTTGAC
HindII 2137 GTTGAC GT[CT]^[GA]AC
3037 GTTGAC
KspI 41 CCGCGG CCGC^GG
146 CCGCGG
NheI 1665 GCTAGC G^CTAGC
1878 GCTAGC
PflMI 1835 CCAGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2401 CCAGGATTTGG
PmaCI 212 CACGTG CAC^GTG
2587 CACGTG
PmlI 212 CACGTG CAC^GTG
2587 CACGTG
PstI 1993 CTGCAG CTGCA^G
2158 CTGCAG
RcaI 1481 TCATGA T^CATGA
3987 TCATGA
SacII 41 CCGCGG CCGC^GG
146 CCGCGG
SapI 355 GAAGAGC GCTCTTC(1/4)
1873 GAAGAGC
ScaI 2500 AGTACT AGT^ACT
3451 AGTACT
SspBI 653 TGTACA T^GTACA
3781 TGTACA
SspI 2382 AATATT AAT^ATT
2647 AATATT
StuI 1556 AGGCCT AGG^CCT
1756 AGGCCT
Van91I 1835 CCAGGGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2401 CCAGGATTTGG
XmnI 3304 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3719 GAAATAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaII
BanI BbsI BbvI BfaI BmyI
BpmI BpuAI BsaBI BsaJI BsaWI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII