Restriction map for
pFN21AE5237
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BpmI
BpuAI BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MaeII MaeIII MfeI
MflI MluI MluNI MroI MscI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tsp45I Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AcsI 591 AAATTT [GA]^AATT[CT]
ApaI 398 GGGCCC GGGCC^C
ApoI 591 AAATTT [GA]^AATT[CT]
AvaII 193 GGTCC G^G[AT]CC
BanII 398 GGGCCC G[GA]GC[CT]^C
BfaI 674 CTAG C^TAG
BlpI 338 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 338 GCTTAGC GC^T[ACGT]AGC
BsaBI 318 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 746 GAGACC GGTCTC(1/5)
BsrDI 343 GCAATG GCAATG(2/0)
BstF5I 30 CATCC GGATG(2/0)
CelII 338 GCTTAGC GC^T[ACGT]AGC
CfoI 45 GCGC GCG^C
EarI 115 CTCTTC CTCTTC(1/4)
FauI 710 GCGGG CCCGC(4/6)
FokI 30 CATCC GGATG(9/13)
HhaI 45 GCGC GCG^C
HinP1I 45 GCGC G^CGC
Ksp632I 115 CTCTTC CTCTTC(1/4)
MaeI 674 CTAG C^TAG
MamI 318 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 673 GCTAGC G^CTAGC
PflMI 196 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 403 CAGCTG CAG^CTG
ScaI 384 AGTACT AGT^ACT
StuI 452 AGGCCT AGG^CCT
TaqI 738 TCGA T^CGA
TspRI 168 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 738 TCGA T^CGA
Van91I 196 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 44 CGCG CG^CG
251 CGCG
AfaI 385 GTAC GT^AC
618 GTAC
AlwNI 197 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
355 CAGTTGCTG
BbvI 627 GCTGC GCAGC(8/12)
688 GCTGC
BclI 374 TGATCA T^GATCA
693 TGATCA
BcnI 33 CCCGG CC^[GC]GG
105 CCCGG
BsiYI 76 CCCTTGGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
196 CCAGGAACTGG
BslI 76 CCCTTGGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
196 CCAGGAACTGG
BstUI 44 CGCG CG^CG
251 CGCG
DdeI 339 CTTAG C^T[ACGT]AG
608 CTCAG
EcoT14I 77 CCTTGG C^C[AT][AT]GG
477 CCTTGG
FbaI 374 TGATCA T^GATCA
693 TGATCA
HapII 34 CCGG C^CGG
106 CCGG
HinfI 366 GATTC G^A[ACGT]TC
482 GATTC
HpaII 34 CCGG C^CGG
106 CCGG
MboII 587 GAAGA GAAGA(8/7)
116 TCTTC
MseI 311 TTAA T^TAA
449 TTAA
MspA1I 403 CAGCTG C[AC]G^C[GT]G
621 CCGCTG
MspI 34 CCGG C^CGG
106 CCGG
MvnI 44 CGCG CG^CG
251 CGCG
NciI 33 CCCGG CC^[GC]GG
105 CCCGG
RsaI 385 GTAC GT^AC
618 GTAC
SfaNI 29 GCATC GCATC(5/9)
323 GCATC
StyI 77 CCTTGG C^C[AT][AT]GG
477 CCTTGG
TfiI 366 GATTC G^A[AT]TC
482 GATTC
Tru9I 311 TTAA T^TAA
449 TTAA
TseI 627 GCTGC G^C[AT]GC
688 GCTGC
Tsp509I 223 AATT ^AATT
592 AATT
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AluI BmyI BsaJI BseRI
BsmAI Bsp1286I BsrI BssKI BstNI
Cac8I Cfr13I DpnII EcoRII Fnu4HI
HaeIII ItaI MboI MnlI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI