Restriction map for
pFN21AE5683
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcyI AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BclI BglII
BlnI BpmI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HindIII KasI KpnI KspI
MluI MluNI MroI MscI MslI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AclI 1545 AACGTT AA^CGTT
AflII 294 CTTAAG C^TTAAG
Asp700I 447 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 148 GGTGCC G^G[CT][GA]CC
BbsI 1300 GTCTTC GAAGAC(2/6)
BbvCI 762 CCTCAGC CCTCAGC(-5/-2)
BciVI 23 GTATCC GTATCC(6/5)
BfrI 294 CTTAAG C^TTAAG
BglI 151 GCCTTTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 963 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 963 GCTGAGC GC^T[ACGT]AGC
BpuAI 1300 GTCTTC GAAGAC(2/6)
BsaAI 508 CACGTA [CT]AC^GT[GA]
BsaBI 390 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1249 GGTCTC GGTCTC(1/5)
BspLU11I 1328 ACATGT A^CATGT
CelII 963 GCTGAGC GC^T[ACGT]AGC
DraI 732 TTTAAA TTT^AAA
DrdI 477 GACAGATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 698 CCATGG C^C[GA][CT]GG
FauI 1345 CCCGC CCCGC(4/6)
HgaI 1581 GACGC GACGC(5/10)
HincII 1230 GTTAAC GT[CT]^[GA]AC
HindII 1230 GTTAAC GT[CT]^[GA]AC
HpaI 1230 GTTAAC GTT^AAC
MamI 390 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 698 CCATGG C^CATGG
PpuMI 465 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1545 AACGTT AA^CGTT
SapI 1188 GCTCTTC GCTCTTC(1/4)
SfcI 1022 CTATAG C^T[GA][CT]AG
SmlI 294 CTTAAG C^T[CT][GA]AG
SphI 1558 GCATGC GCATG^C
Tsp45I 486 GTCAC ^GT[GC]AC
XmnI 447 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AciI 1346 CCGC CCGC(-3/-1)
1617 GCGG
AcsI 907 GAATTT [GA]^AATT[CT]
1205 AAATTT
AflIII 507 ACACGT A^C[GA][CT]GT
1328 ACATGT
ApoI 907 GAATTT [GA]^AATT[CT]
1205 AAATTT
AspHI 199 GAGCAC G[AT]GC[AT]^C
596 GAGCAC
AvaII 466 GGTCC G^G[AT]CC
844 GGACC
BcgI 953 CGAACCGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
997 CGATCTAAGTGC
BfaI 223 CTAG C^TAG
1243 CTAG
BseRI 64 CTCCTC GAGGAG(10/8)
1219 CTCCTC
BsiHKAI 199 GAGCAC G[AT]GC[AT]^C
596 GAGCAC
BsrDI 1170 CATTGC GCAATG(2/0)
1096 GCAATG
DraII 465 GGGTCCC [GA]G^G[ACGT]CC[CT]
802 AGGGCCT
EarI 1189 CTCTTC CTCTTC(1/4)
1222 CTCTTC
EcoO109I 465 GGGTCCC [GA]G^G[ACGT]CC[CT]
802 AGGGCCT
EcoT14I 69 CCTTGG C^C[AT][AT]GG
698 CCATGG
EcoT22I 1 ATGCAT ATGCA^T
1556 ATGCAT
HaeIII 804 GGCC GG^CC
1505 GGCC
Ksp632I 1189 CTCTTC CTCTTC(1/4)
1222 CTCTTC
MaeI 223 CTAG C^TAG
1243 CTAG
MaeII 509 ACGT A^CGT
1546 ACGT
MaeIII 486 GTCAC ^GT[ACGT]AC
511 GTAAC
MfeI 517 CAATTG C^AATTG
1361 CAATTG
MspA1I 80 CAGCTG C[AC]G^C[GT]G
1272 CAGCTG
MunI 517 CAATTG C^AATTG
1361 CAATTG
NsiI 1 ATGCAT ATGCA^T
1556 ATGCAT
NspI 1328 ACATGT [GA]CATG^[CT]
1558 GCATGC
PvuII 80 CAGCTG CAG^CTG
1272 CAGCTG
StyI 69 CCTTGG C^C[AT][AT]GG
698 CCATGG
TaqI 996 TCGA T^CGA
1333 TCGA
TthHB8I 996 TCGA T^CGA
1333 TCGA
XcmI 921 CCAATGTAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1438 CCAGTATCAGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AfaI AluI AlwI BbvI BcnI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HapII HinfI HpaII HphI
ItaI MboI MboII MflI MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SspBI
TfiI Tru9I TseI Tsp509I TspRI
XhoII