Restriction map for
pFN21AB5932
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# NO CUT ENZYMES ( 112 enzymes) #
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AatII AccI AccIII AcsI AflII
AflIII AgeI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspHI AspI AviII AvrII
BalI BanII BbrPI BcgI BclI
BfaI BfrI BglI BlnI BseAI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MaeI MluI
MluNI MroI MscI MslI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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Acc65I 1207 GGTACC G^GTACC
AclI 886 AACGTT AA^CGTT
AhdI 682 GACCCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 352 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 831 CCTAAGG CC^T[ACGT]AGG
Asp718I 1207 GGTACC G^GTACC
AspEI 682 GACCCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 610 GGATCC G^GATCC
BbeI 211 GGCGCC GGCGC^C
BbsI 1464 GTCTTC GAAGAC(2/6)
BbvCI 916 GCTGAGG CCTCAGC(-5/-2)
BciVI 619 GTATCC GTATCC(6/5)
BglII 422 AGATCT A^GATCT
BlpI 814 GCTAAGC GC^T[ACGT]AGC
BpmI 806 CTCCAG CTGGAG(16/14)
Bpu1102I 814 GCTAAGC GC^T[ACGT]AGC
BpuAI 1464 GTCTTC GAAGAC(2/6)
BsaAI 463 TACGTG [CT]AC^GT[GA]
BsaWI 719 ACCGGA [AT]^CCGG[AT]
BseRI 151 GAGGAG GAGGAG(10/8)
BsgI 529 GTGCAG GTGCAG(16/14)
BsiEI 51 CGGCCG CG[GA][CT]^CG
BsrBI 66 GAGCGG CCGCTC(-3/-3)
BsrFI 33 GCCGGC [GA]^CCGG[CT]
BstAPI 1328 GCACTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 831 CCTAAGG CC^T[ACGT]AGG
CelII 814 GCTAAGC GC^T[ACGT]AGC
Cfr10I 33 GCCGGC [GA]^CCGG[CT]
DraII 1256 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 820 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 51 CGGCCG C^GGCCG
Eam1105I 682 GACCCCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 51 CGGCCG C^GGCCG
Eco52I 51 CGGCCG C^GGCCG
Eco81I 831 CCTAAGG CC^T[ACGT]AGG
EcoNI 658 CCTCGGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1256 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 767 CCTTGG C^C[AT][AT]GG
KasI 211 GGCGCC G^GCGCC
KpnI 1207 GGTACC GGTAC^C
MaeIII 751 GTCAC ^GT[ACGT]AC
MfeI 1553 CAATTG C^AATTG
MunI 1553 CAATTG C^AATTG
NaeI 33 GCCGGC GCC^GGC
NarI 211 GGCGCC GG^CGCC
NgoMIV 33 GCCGGC G^CCGGC
PpuMI 1256 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 886 AACGTT AA^CGTT
PstI 204 CTGCAG CTGCA^G
PvuII 931 CAGCTG CAG^CTG
SfcI 204 CTGCAG C^T[GA][CT]AG
SfoI 211 GGCGCC GGC^GCC
SmaI 228 CCCGGG CCC^GGG
SmlI 120 CTTGAG C^T[CT][GA]AG
SphI 1073 GCATGC GCATG^C
StyI 767 CCTTGG C^C[AT][AT]GG
TaqI 319 TCGA T^CGA
Tsp45I 751 GTCAC ^GT[GC]AC
TthHB8I 319 TCGA T^CGA
XmaCI 228 CCCGGG C^CCGGG
XmaI 228 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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BmyI 223 GGGCAC G[AGT]GC[ACT]^C
304 GTGCCC
BsaBI 372 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
402 GATGCCCATC
BsaI 716 GAGACC GGTCTC(1/5)
1246 GAGACC
Bsp1286I 223 GGGCAC G[AGT]GC[ACT]^C
304 GTGCCC
BsrDI 948 CATTGC GCAATG(2/0)
1314 CATTGC
BssHII 242 GCGCGC G^CGCGC
543 GCGCGC
BstXI 324 CCAGAAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1130 CCACCAGGCTGG
DsaI 851 CCACGG C^C[GA][CT]GG
1078 CCACGG
EaeI 51 CGGCCG [CT]^GGCC[GA]
770 TGGCCG
EarI 1121 CTCTTC CTCTTC(1/4)
1234 CTCTTC
HaeII 157 GGCGCT [GA]GCGC^[CT]
211 GGCGCC
Ksp632I 1121 CTCTTC CTCTTC(1/4)
1234 CTCTTC
MamI 372 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
402 GATGCCCATC
MseI 890 TTAA T^TAA
1060 TTAA
MspA1I 61 CAGCGG C[AC]G^C[GT]G
931 CAGCTG
NspI 578 ACATGC [GA]CATG^[CT]
1073 GCATGC
Tru9I 890 TTAA T^TAA
1060 TTAA
Tsp509I 1300 AATT ^AATT
1554 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaI AvaII BanI BbvI
BcnI BsaHI BsaJI BsiYI BslI
BsmAI BsmFI BsoBI BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MflI MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI TseI
TspRI XhoII