Restriction map for
pFN21AE5453
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspHI AspI AviII AvrII
BalI BamHI BanII BbeI BbsI
BcgI BciVI BfrI BglI BglII
BlnI BpmI BpuAI BsaBI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspMI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KasI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SmlI SnaBI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp45I Tth111I TthHB8I Van91I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 19 GGTACC G^GTACC
AcsI 618 AAATTC [GA]^AATT[CT]
AcyI 3 GGCGTC G[GA]^CG[CT]C
AflIII 587 ACATGT A^C[GA][CT]GT
AlwNI 730 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 889 CCTGAGG CC^T[ACGT]AGG
ApoI 618 AAATTC [GA]^AATT[CT]
Asp700I 181 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 19 GGTACC G^GTACC
AvaII 897 GGACC G^G[AT]CC
BanI 19 GGTACC G^G[CT][GA]CC
BbrPI 346 CACGTG CAC^GTG
BbvCI 253 CCTCAGC CCTCAGC(-5/-2)
BfaI 800 CTAG C^TAG
BlpI 690 GCTCAGC GC^T[ACGT]AGC
BmyI 392 GGGCAC G[AGT]GC[ACT]^C
Bpu1102I 690 GCTCAGC GC^T[ACGT]AGC
BsaAI 346 CACGTG [CT]AC^GT[GA]
BsaHI 3 GGCGTC G[GA]^CG[CT]C
BsgI 115 GTGCAG GTGCAG(16/14)
BsmFI 6 GTCCC GGGAC(10/14)
Bsp1286I 392 GGGCAC G[AGT]GC[ACT]^C
BspLU11I 587 ACATGT A^CATGT
BsrBI 883 CCGCTC CCGCTC(-3/-3)
BsrDI 954 CATTGC GCAATG(2/0)
BstYI 478 GGATCT [GA]^GATC[CT]
Bsu36I 889 CCTGAGG CC^T[ACGT]AGG
CelII 690 GCTCAGC GC^T[ACGT]AGC
DraII 896 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 889 CCTGAGG CC^T[ACGT]AGG
EcoO109I 896 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 474 CCAAGG C^C[AT][AT]GG
HaeII 860 AGCGCC [GA]GCGC^[CT]
Hin1I 3 GGCGTC G[GA]^CG[CT]C
HphI 575 TCACC GGTGA(8/7)
KpnI 19 GGTACC GGTAC^C
MaeI 800 CTAG C^TAG
MaeII 347 ACGT A^CGT
MflI 478 GGATCT [GA]^GATC[CT]
MslI 783 CACATACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 665 CAGCTG C[AC]G^C[GT]G
NaeI 55 GCCGGC GCC^GGC
NgoMIV 55 GCCGGC G^CCGGC
PmaCI 346 CACGTG CAC^GTG
PmlI 346 CACGTG CAC^GTG
PpuMI 896 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 665 CAGCTG CAG^CTG
SfcI 727 CTACAG C^T[GA][CT]AG
SgrAI 54 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 389 CCCGGG CCC^GGG
SpeI 799 ACTAGT A^CTAGT
SphI 423 GCATGC GCATG^C
StyI 474 CCAAGG C^C[AT][AT]GG
TspRI 999 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 478 GGATCT [GA]^GATC[CT]
XmaCI 389 CCCGGG C^CCGGG
XmaI 389 CCCGGG C^CCGGG
XmnI 181 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 20 GTAC GT^AC
585 GTAC
AlwI 457 GATCC GGATC(4/5)
478 GGATC
AvaI 90 CTCGGG C^[CT]CG[GA]G
389 CCCGGG
BclI 336 TGATCA T^GATCA
629 TGATCA
BcnI 389 CCCGG CC^[GC]GG
390 CCGGG
BseRI 872 GAGGAG GAGGAG(10/8)
892 GAGGAG
BsiYI 639 CCTAATGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
888 CCCTGAGGAGG
BslI 639 CCTAATGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
888 CCCTGAGGAGG
BsoBI 90 CTCGGG C^[CT]CG[GA]G
389 CCCGGG
BsrFI 22 ACCGGC [GA]^CCGG[CT]
55 GCCGGC
CfoI 59 GCGC GCG^C
861 GCGC
Cfr10I 22 ACCGGC [GA]^CCGG[CT]
55 GCCGGC
Cfr13I 897 GGACC G^G[ACGT]CC
1029 GGCCC
FauI 8 CCCGC CCCGC(4/6)
882 CCCGC
FbaI 336 TGATCA T^GATCA
629 TGATCA
HgaI 4 GCGTC GACGC(5/10)
837 GCGTC
HhaI 59 GCGC GCG^C
861 GCGC
HinP1I 59 GCGC G^CGC
861 GCGC
MaeIII 385 GTTAC ^GT[ACGT]AC
416 GTTAC
NciI 389 CCCGG CC^[GC]GG
390 CCGGG
NlaIV 19 GGTACC GG[ACGT]^[ACGT]CC
314 GGAGCC
NspI 423 GCATGC [GA]CATG^[CT]
587 ACATGT
RsaI 20 GTAC GT^AC
585 GTAC
SapI 1020 GCTCTTC GCTCTTC(1/4)
1036 GCTCTTC
Sau96I 897 GGACC G^G[ACGT]CC
1029 GGCCC
SfaNI 271 GATGC GCATC(5/9)
309 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BbvI BsaJI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII PleI
Sau3AI ScrFI TfiI Tru9I TseI
Tsp509I