Restriction map for
pFN21AE3441
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
Aor51HI AscI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbsI
BbvCI BcgI BclI BfrI BlnI
BpuAI BsaBI BsiEI BsiWI BsmBI
BspDI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Cfr10I ClaI
CpoI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KpnI KspI MamI MluNI
MscI NaeI NgoMIV NheI NotI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PvuI RcaI RsrII
SacII SalI SapI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspI SwaI Tth111I XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 2275 GTCTAC GT^[AC][GT]AC
AccIII 44 TCCGGA T^CCGGA
AclI 70 AACGTT AA^CGTT
AflIII 2792 ACGCGT A^C[GA][CT]GT
ApaI 2630 GGGCCC GGGCC^C
AseI 1480 ATTAAT AT^TAAT
AsnI 1480 ATTAAT AT^TAAT
AvaI 1542 CTCGAG C^[CT]CG[GA]G
BbeI 2604 GGCGCC GGCGC^C
BbrPI 3427 CACGTG CAC^GTG
BciVI 2646 GTATCC GTATCC(6/5)
BcnI 672 CCCGG CC^[GC]GG
BglI 2545 GCCCATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 750 AGATCT A^GATCT
BsaAI 3427 CACGTG [CT]AC^GT[GA]
BsaI 3255 GAGACC GGTCTC(1/5)
BseAI 44 TCCGGA T^CCGGA
BsoBI 1542 CTCGAG C^[CT]CG[GA]G
Bsp1407I 3336 TGTACA T^GTACA
BspEI 44 TCCGGA T^CCGGA
BsrGI 3336 TGTACA T^GTACA
DraIII 539 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
FauI 2439 GCGGG CCCGC(4/6)
HgaI 2481 GACGC GACGC(5/10)
HincII 883 GTCAAC GT[CT]^[GA]AC
HindII 883 GTCAAC GT[CT]^[GA]AC
KasI 2604 GGCGCC G^GCGCC
MluI 2792 ACGCGT A^CGCGT
MroI 44 TCCGGA T^CCGGA
MslI 1059 CACTTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2604 GGCGCC GG^CGCC
NciI 672 CCCGG CC^[GC]GG
NruI 1867 TCGCGA TCG^CGA
NspI 225 GCATGT [GA]CATG^[CT]
PaeR7I 1542 CTCGAG C^TCGAG
PmaCI 3427 CACGTG CAC^GTG
PmlI 3427 CACGTG CAC^GTG
PshBI 1480 ATTAAT AT^TAAT
Psp1406I 70 AACGTT AA^CGTT
SacI 1539 GAGCTC GAGCT^C
SbfI 2595 CCTGCAGG CCTGCA^GG
SfoI 2604 GGCGCC GGC^GCC
Sse8387I 2595 CCTGCAGG CCTGCA^GG
SspBI 3336 TGTACA T^GTACA
XcmI 3747 CCATGGTGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1542 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 2481 GACGCC G[GA]^CG[CT]C
2604 GGCGCC
Alw44I 1823 GTGCAC G^TGCAC
3691 GTGCAC
AocI 467 CCTCAGG CC^T[ACGT]AGG
3163 CCTCAGG
ApaLI 1823 GTGCAC G^TGCAC
3691 GTGCAC
Asp700I 1560 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3188 GAAATAGTTC
BsaHI 2481 GACGCC G[GA]^CG[CT]C
2604 GGCGCC
BsaWI 44 TCCGGA [AT]^CCGG[AT]
2713 TCCGGT
BsmI 2766 GAATGC GAATGC(1/-1)
3546 GCATTC
BsrDI 2408 GCAATG GCAATG(2/0)
2948 GCAATG
Bsu36I 467 CCTCAGG CC^T[ACGT]AGG
3163 CCTCAGG
DraI 703 TTTAAA TTT^AAA
2835 TTTAAA
DrdI 2266 GACTTCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2481 GACGCCTGTGTC
DsaI 1644 CCATGG C^C[GA][CT]GG
3747 CCATGG
Eco81I 467 CCTCAGG CC^T[ACGT]AGG
3163 CCTCAGG
HaeII 2604 GGCGCC [GA]GCGC^[CT]
2891 AGCGCC
Hin1I 2481 GACGCC G[GA]^CG[CT]C
2604 GGCGCC
MfeI 555 CAATTG C^AATTG
2730 CAATTG
MunI 555 CAATTG C^AATTG
2730 CAATTG
NcoI 1644 CCATGG C^CATGG
3747 CCATGG
NdeI 3736 CATATG CA^TATG
3777 CATATG
PpuMI 949 GGGACCT [GA]G^G[AT]CC[CT]
3621 AGGTCCC
ScaI 3172 AGTACT AGT^ACT
3661 AGTACT
StuI 931 AGGCCT AGG^CCT
2830 AGGCCT
XbaI 1306 TCTAGA T^CTAGA
3528 TCTAGA
XmnI 1560 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3188 GAAATAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbvI BfaI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII