Restriction map for
pFN21AE9568
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT14I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StyI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AciI 230 CCGC CCGC(-3/-1)
AlwNI 203 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 325 ATTAAT AT^TAAT
AsnI 325 ATTAAT AT^TAAT
AvaII 170 GGACC G^G[AT]CC
BbvI 985 GCAGC GCAGC(8/12)
BciVI 1177 GTATCC GTATCC(6/5)
BcnI 948 CCCGG CC^[GC]GG
BglII 545 AGATCT A^GATCT
BsaBI 432 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 10 GAGAC GTCTC(1/5)
BsmFI 969 GTCCC GGGAC(10/14)
BspHI 149 TCATGA T^CATGA
BssSI 47 CACGAG CACGAG(-5/-1)
BstAPI 914 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 794 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 169 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 583 GACTTTGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 227 TGGCCG [CT]^GGCC[GA]
EcoO109I 169 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 329 ATGCAT ATGCA^T
HapII 949 CCGG C^CGG
HgaI 1064 GCGTC GACGC(5/10)
HincII 862 GTCAAC GT[CT]^[GA]AC
HindII 862 GTCAAC GT[CT]^[GA]AC
HpaII 949 CCGG C^CGG
MamI 432 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 949 CCGG C^CGG
NciI 948 CCCGG CC^[GC]GG
NsiI 329 ATGCAT ATGCA^T
PflMI 312 CCAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 169 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 325 ATTAAT AT^TAAT
RcaI 149 TCATGA T^CATGA
SspI 499 AATATT AAT^ATT
StuI 234 AGGCCT AGG^CCT
TseI 985 GCAGC G^C[AT]GC
Van91I 312 CCAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 917 GAATTT [GA]^AATT[CT]
976 AAATTC
AfaI 94 GTAC GT^AC
369 GTAC
ApoI 917 GAATTT [GA]^AATT[CT]
976 AAATTC
BfaI 644 CTAG C^TAG
853 CTAG
BsaJI 111 CCTGGG C^C[ACGT][ACGT]GG
269 CCTGGG
BsgI 408 CTGCAC GTGCAG(16/14)
983 GTGCAG
BstEII 78 GGTCACC G^GT[ACGT]ACC
789 GGTCACC
BstPI 78 GGTCACC G^GT[ACGT]ACC
789 GGTCACC
Cfr13I 170 GGACC G^G[ACGT]CC
1050 GGCCC
EcoO65I 78 GGTCACC G^GT[ACGT]ACC
789 GGTCACC
EcoRV 528 GATATC GAT^ATC
1105 GATATC
Fnu4HI 229 GCCGC GC^[ACGT]GC
985 GCAGC
ItaI 229 GCCGC GC^[ACGT]GC
985 GCAGC
MaeI 644 CTAG C^TAG
853 CTAG
MboII 482 TCTTC GAAGA(8/7)
775 TCTTC
MseI 326 TTAA T^TAA
658 TTAA
MslI 792 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1137 CACAAAAGTG
NlaIV 170 GGACCC GG[ACGT]^[ACGT]CC
265 GGTTCC
PleI 849 GACTC GAGTC(4/5)
1075 GAGTC
RsaI 94 GTAC GT^AC
369 GTAC
Sau96I 170 GGACC G^G[ACGT]CC
1050 GGCCC
SexAI 71 ACCAGGT A^CC[AT]GGT
188 ACCTGGT
SfcI 843 CTACAG C^T[GA][CT]AG
1041 CTACAG
Tru9I 326 TTAA T^TAA
658 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AlwI BpmI BsiYI BslI
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII EcoRII
FokI HaeIII HinfI HphI MaeIII
MboI MflI MnlI MvaI MwoI
NdeII NlaIII Sau3AI ScrFI SfaNI
TfiI Tsp45I Tsp509I TspRI XhoII