##################################
# NO CUT ENZYMES (  212 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstEII      
BstNI       BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       HphI        KasI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MunI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NlaIV       NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        ScrFI       SexAI       SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AcsI          261  AAATTT               [GA]^AATT[CT]
ApoI          261  AAATTT               [GA]^AATT[CT]
BbsI          146  GAAGAC               GAAGAC(2/6)
BbvI           70  GCTGC                GCAGC(8/12)
BglII         221  AGATCT               A^GATCT
BmyI          194  GGGCAC               G[AGT]GC[ACT]^C
BpuAI         146  GAAGAC               GAAGAC(2/6)
Bsp1286I      194  GGGCAC               G[AGT]GC[ACT]^C
BstBI          50  TTCGAA               TT^CGAA
BstYI         221  AGATCT               [GA]^GATC[CT]
Cac8I          12  GCAGGC               GC[ACGT]^[ACGT]GC
DdeI          155  CTGAG                C^T[ACGT]AG
DpnII         222  GATC                 ^GATC
EarI          253  GAAGAG               CTCTTC(1/4)
Fnu4HI         70  GCTGC                GC^[ACGT]GC
HaeIII         15  GGCC                 GG^CC
HinfI          77  GATTC                G^A[ACGT]TC
ItaI           70  GCTGC                GC^[ACGT]GC
Ksp632I       253  GAAGAG               CTCTTC(1/4)
MboI          222  GATC                 ^GATC
MflI          221  AGATCT               [GA]^GATC[CT]
NdeII         222  GATC                 ^GATC
NspV           50  TTCGAA               TT^CGAA
PstI           71  CTGCAG               CTGCA^G
Sau3AI        222  GATC                 ^GATC
SfcI           71  CTGCAG               C^T[GA][CT]AG
SfuI           50  TTCGAA               TT^CGAA
TaqI           51  TCGA                 T^CGA
TfiI           77  GATTC                G^A[AT]TC
TseI           70  GCTGC                G^C[AT]GC
Tsp509I       262  AATT                 ^AATT
TthHB8I        51  TCGA                 T^CGA
XhoII         221  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

AvaII         201  GGACC                G^G[AT]CC
              242  GGACC               
BsrI           87  ACTGG                ACTGG(1/-1)
              198  ACTGG               
Cfr13I        201  GGACC                G^G[ACGT]CC
              242  GGACC               
MseI           94  TTAA                 T^TAA
              188  TTAA                
MwoI           16  GCCGTTTCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              228  GCCTAAAGAGC         
Sau96I        201  GGACC                G^G[ACGT]CC
              242  GGACC               
SfaNI          25  GCATC                GCATC(5/9)
              216  GCATC               
Tru9I          94  TTAA                 T^TAA
              188  TTAA                
TspRI         197  CACTG                CA[GC]TG[ACGT][ACGT]^
              238  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

BstF5I      FokI        MboII       MnlI