Restriction map for
pFN21AE6502
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BseRI
BsgI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MslI MspA1I MunI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 484 GAATTT [GA]^AATT[CT]
AfaI 321 GTAC GT^AC
ApoI 484 GAATTT [GA]^AATT[CT]
AseI 199 ATTAAT AT^TAAT
AsnI 199 ATTAAT AT^TAAT
AvaII 9 GGACC G^G[AT]CC
BanI 106 GGCACC G^G[CT][GA]CC
BpmI 552 CTGGAG CTGGAG(16/14)
BsiEI 120 CGACCG CG[GA][CT]^CG
BsiYI 129 CCAAGCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 129 CCAAGCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 145 GAGAC GTCTC(1/5)
BsmBI 145 GAGACG CGTCTC(1/5)
BsmFI 440 GGGAC GGGAC(10/14)
BsrI 543 CCAGT ACTGG(1/-1)
BssKI 12 CCTGG ^CC[ACGT]GG
BstF5I 27 GGATG GGATG(2/0)
BstNI 12 CCTGG CC^[AT]GG
Cac8I 538 GCCTGC GC[ACGT]^[ACGT]GC
Cfr13I 9 GGACC G^G[ACGT]CC
DraI 169 TTTAAA TTT^AAA
DraII 8 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 214 GAAGAG CTCTTC(1/4)
EcoO109I 8 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 12 CCTGG ^CC[AT]GG
FokI 27 GGATG GGATG(9/13)
HaeIII 295 GGCC GG^CC
HgaI 147 GACGC GACGC(5/10)
HinfI 37 GAGTC G^A[ACGT]TC
Ksp632I 214 GAAGAG CTCTTC(1/4)
MvaI 12 CCTGG CC^[AT]GG
NdeI 533 CATATG CA^TATG
PleI 37 GAGTC GAGTC(4/5)
PpuMI 8 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 199 ATTAAT AT^TAAT
RsaI 321 GTAC GT^AC
SapI 214 GAAGAGC GCTCTTC(1/4)
Sau96I 9 GGACC G^G[ACGT]CC
ScrFI 12 CCTGG CC^[ACGT]GG
SspI 361 AATATT AAT^ATT
StuI 294 AGGCCT AGG^CCT
TspRI 544 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AspHI 19 GAGCTC G[AT]GC[AT]^C
333 GAGCTC
BbvI 84 GCAGC GCAGC(8/12)
95 GCTGC
BsaWI 122 ACCGGA [AT]^CCGG[AT]
315 TCCGGT
BsiHKAI 19 GAGCTC G[AT]GC[AT]^C
333 GAGCTC
DdeI 64 CTAAG C^T[ACGT]AG
151 CTCAG
HapII 123 CCGG C^CGG
316 CCGG
HpaII 123 CCGG C^CGG
316 CCGG
MnlI 239 CCTC CCTC(7/6)
7 GAGG
MspI 123 CCGG C^CGG
316 CCGG
NlaIV 106 GGCACC GG[ACGT]^[ACGT]CC
125 GGAACC
NspI 376 GCATGT [GA]CATG^[CT]
476 ACATGC
SacI 19 GAGCTC GAGCT^C
333 GAGCTC
TaqI 35 TCGA T^CGA
80 TCGA
TseI 84 GCAGC G^C[AT]GC
95 GCTGC
Tsp45I 380 GTGAC ^GT[GC]AC
571 GTGAC
TthHB8I 35 TCGA T^CGA
80 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AluI BanII BmyI BsaJI
Bsp1286I DpnII EcoT14I Fnu4HI ItaI
MaeIII MboI MboII MseI MwoI
NdeII NlaIII Sau3AI StyI Tru9I
Tsp509I