Restriction map for
pFN21AE3130
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AvaII
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmFI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstF5I BstPI BstYI
Bsu36I CelII Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoT14I
FauI FbaI FokI FseI FspI
HaeII HgaI Hin1I HindIII KasI
KspI MaeII MamI MflI MluI
MroI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI Sau96I SbfI SfcI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TaqI Tsp45I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 77 GGTACC G^GTACC
AccI 708 GTATAC GT^[AC][GT]AC
AccII 65 CGCG CG^CG
AlwNI 130 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 77 GGTACC G^GTACC
AvaI 8 CCCGGG C^[CT]CG[GA]G
BbsI 664 GAAGAC GAAGAC(2/6)
BpuAI 664 GAAGAC GAAGAC(2/6)
BsgI 48 GTGCAG GTGCAG(16/14)
BsmBI 399 GAGACG CGTCTC(1/5)
BsmI 192 GAATGC GAATGC(1/-1)
BsoBI 8 CCCGGG C^[CT]CG[GA]G
BspMI 50 GCAGGT ACCTGC(4/8)
BsrFI 44 GCCGGT [GA]^CCGG[CT]
BsrI 288 ACTGG ACTGG(1/-1)
Bst1107I 708 GTATAC GTA^TAC
BstNI 81 CCTGG CC^[AT]GG
BstUI 65 CGCG CG^CG
BstXI 479 CCAGACATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 708 GTATAC GTA^TAC
Cac8I 168 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 64 GCGC GCG^C
Cfr10I 44 GCCGGT [GA]^CCGG[CT]
DpnII 583 GATC ^GATC
EcoNI 691 CCTAATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 81 CCTGG ^CC[AT]GG
EcoRV 381 GATATC GAT^ATC
EcoT22I 899 ATGCAT ATGCA^T
HhaI 64 GCGC GCG^C
HinP1I 64 GCGC G^CGC
HincII 340 GTTAAC GT[CT]^[GA]AC
HindII 340 GTTAAC GT[CT]^[GA]AC
HpaI 340 GTTAAC GTT^AAC
KpnI 77 GGTACC GGTAC^C
MboI 583 GATC ^GATC
MfeI 295 CAATTG C^AATTG
MslI 898 CATGCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 295 CAATTG C^AATTG
MvaI 81 CCTGG CC^[AT]GG
MvnI 65 CGCG CG^CG
NdeII 583 GATC ^GATC
NsiI 899 ATGCAT ATGCA^T
NspI 897 ACATGC [GA]CATG^[CT]
PflMI 547 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 785 GAGCTC GAGCT^C
Sau3AI 583 GATC ^GATC
ScaI 731 AGTACT AGT^ACT
SexAI 80 ACCTGGT A^CC[AT]GGT
SfaNI 144 GCATC GCATC(5/9)
SmaI 8 CCCGGG CCC^GGG
TspRI 559 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 547 CCATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 8 CCCGGG C^CCGGG
XmaI 8 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 638 CCGC CCGC(-3/-1)
97 GCGG
AcsI 176 AAATTC [GA]^AATT[CT]
721 GAATTT
AfaI 78 GTAC GT^AC
732 GTAC
ApoI 176 AAATTC [GA]^AATT[CT]
721 GAATTT
Asp700I 664 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
718 GAAGAATTTC
AspHI 605 GTGCTC G[AT]GC[AT]^C
785 GAGCTC
BalI 245 TGGCCA TGG^CCA
555 TGGCCA
BanI 33 GGTGCC G^G[CT][GA]CC
77 GGTACC
BanII 4 GAGCCC G[GA]GC[CT]^C
785 GAGCTC
BcnI 8 CCCGG CC^[GC]GG
9 CCGGG
BpmI 713 CTCCAG CTGGAG(16/14)
289 CTGGAG
BseRI 836 CTCCTC GAGGAG(10/8)
219 GAGGAG
BsiHKAI 605 GTGCTC G[AT]GC[AT]^C
785 GAGCTC
EaeI 245 TGGCCA [CT]^GGCC[GA]
555 TGGCCA
HaeIII 246 GGCC GG^CC
556 GGCC
HapII 9 CCGG C^CGG
45 CCGG
HpaII 9 CCGG C^CGG
45 CCGG
MaeIII 931 GTTAC ^GT[ACGT]AC
952 GTTAC
MluNI 245 TGGCCA TGG^CCA
555 TGGCCA
MscI 245 TGGCCA TGG^CCA
555 TGGCCA
MspI 9 CCGG C^CGG
45 CCGG
MwoI 135 GCTGAGAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
294 GCAATTGAAGC
NciI 8 CCCGG CC^[GC]GG
9 CCGGG
NdeI 484 CATATG CA^TATG
902 CATATG
PvuII 130 CAGCTG CAG^CTG
755 CAGCTG
RsaI 78 GTAC GT^AC
732 GTAC
SapI 109 GAAGAGC GCTCTTC(1/4)
124 GAAGAGC
SspI 510 AATATT AAT^ATT
860 AATATT
XmnI 664 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
718 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AluI BbvI BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BssKI
DdeI EarI Fnu4HI HinfI HphI
ItaI Ksp632I MaeI MboII MnlI
MseI MspA1I NlaIII NlaIV ScrFI
SmlI TfiI Tru9I TseI Tsp509I