Restriction map for
pFN21AE1620
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AflII AgeI AhdI AluI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AvaI AvaII AviII
AvrII BamHI BanII BbeI BbrPI
BbsI BbvCI BbvI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaJI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI Fnu4HI FseI FspI HaeII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaI HpaII ItaI
KasI Ksp632I KspI MaeI MaeII
MamI MboII MfeI MflI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
TseI TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 19 GGTACC G^GTACC
AcsI 208 GAATTT [GA]^AATT[CT]
AcyI 364 GGCGTC G[GA]^CG[CT]C
AflIII 279 ACATGT A^C[GA][CT]GT
ApoI 208 GAATTT [GA]^AATT[CT]
Asp718I 19 GGTACC G^GTACC
AspHI 246 GAGCAC G[AT]GC[AT]^C
AspI 214 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 107 TGGCCA TGG^CCA
BanI 19 GGTACC G^G[CT][GA]CC
BcgI 216 CGAAGTCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 246 GAGCAC G[AGT]GC[ACT]^C
BpmI 36 CTCCAG CTGGAG(16/14)
BsaHI 364 GGCGTC G[GA]^CG[CT]C
BsgI 283 GTGCAG GTGCAG(16/14)
BsiHKAI 246 GAGCAC G[AT]GC[AT]^C
BsiYI 257 CCTTAGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 257 CCTTAGATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 34 GTCTC GTCTC(1/5)
BsmI 349 GAATGC GAATGC(1/-1)
Bsp1286I 246 GAGCAC G[AGT]GC[ACT]^C
BspHI 356 TCATGA T^CATGA
BspLU11I 279 ACATGT A^CATGT
BssSI 375 CACGAG CACGAG(-5/-1)
BstXI 89 CCAGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 100 GCTGGC GC[ACGT]^[ACGT]GC
Cfr13I 201 GGGCC G^G[ACGT]CC
DraI 172 TTTAAA TTT^AAA
DrdI 232 GACAGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 107 TGGCCA [CT]^GGCC[GA]
Hin1I 364 GGCGTC G[GA]^CG[CT]C
HphI 149 TCACC GGTGA(8/7)
KpnI 19 GGTACC GGTAC^C
MluNI 107 TGGCCA TGG^CCA
MnlI 328 GAGG CCTC(7/6)
MscI 107 TGGCCA TGG^CCA
MslI 126 CATCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 100 GCTGGCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 19 GGTACC GG[ACGT]^[ACGT]CC
NspI 279 ACATGT [GA]CATG^[CT]
PflFI 214 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 224 CTGCAG CTGCA^G
RcaI 356 TCATGA T^CATGA
Sau96I 201 GGGCC G^G[ACGT]CC
SfaNI 7 GATGC GCATC(5/9)
SfcI 224 CTGCAG C^T[GA][CT]AG
Tsp509I 209 AATT ^AATT
Tth111I 214 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 86 CCACCAGGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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BclI 122 TGATCA T^GATCA
146 TGATCA
DdeI 182 CTGAG C^T[ACGT]AG
258 CTTAG
DpnII 123 GATC ^GATC
147 GATC
FbaI 122 TGATCA T^GATCA
146 TGATCA
HaeIII 108 GGCC GG^CC
202 GGCC
HgaI 353 GCGTC GACGC(5/10)
365 GCGTC
MaeIII 220 GTCAC ^GT[ACGT]AC
367 GTCAC
MboI 123 GATC ^GATC
147 GATC
MseI 155 TTAA T^TAA
173 TTAA
NdeII 123 GATC ^GATC
147 GATC
Sau3AI 123 GATC ^GATC
147 GATC
Tru9I 155 TTAA T^TAA
173 TTAA
Tsp45I 220 GTCAC ^GT[GC]AC
367 GTCAC
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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AfaI BsrI BssKI BstF5I BstNI
EcoRII FokI MvaI NlaIII RsaI
ScrFI TspRI