##################################
# NO CUT ENZYMES (  175 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsmBI       BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstEII      BstF5I      BstPI       BstUI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRV       EcoT14I     
EcoT22I     FokI        FseI        FspI        HapII       
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
PacI        PaeR7I      PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StyI        
SwaI        Tsp45I      TspRI       Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AspI          660  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         103  GGACC                G^G[AT]CC
BclI          291  TGATCA               T^GATCA
BfaI          737  CTAG                 C^TAG
BsaAI         437  CACGTA               [CT]AC^GT[GA]
BsmAI         666  GTCTC                GTCTC(1/5)
BsmI          956  GCATTC               GAATGC(1/-1)
BsrDI         261  CATTGC               GCAATG(2/0)
BstBI         959  TTCGAA               TT^CGAA
BstYI         556  GGATCT               [GA]^GATC[CT]
Cac8I         586  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI          584  GCGC                 GCG^C
DdeI          794  CTGAG                C^T[ACGT]AG
DraII         296  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EarI           13  GAAGAG               CTCTTC(1/4)
EcoO109I      296  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         256  GAATTC               G^AATTC
FauI          514  CCCGC                CCCGC(4/6)
FbaI          291  TGATCA               T^GATCA
HaeII         583  GGCGCT               [GA]GCGC^[CT]
HhaI          584  GCGC                 GCG^C
HinP1I        584  GCGC                 G^CGC
HphI          874  TCACC                GGTGA(8/7)
Ksp632I        13  GAAGAG               CTCTTC(1/4)
MaeI          737  CTAG                 C^TAG
MaeII         438  ACGT                 A^CGT
MaeIII         48  GTTAC                ^GT[ACGT]AC
MflI          556  GGATCT               [GA]^GATC[CT]
MslI          901  CATGAGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        398  CAGCTG               C[AC]G^C[GT]G
MwoI          691  GCCCCTCATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV         103  GGACCC               GG[ACGT]^[ACGT]CC
NspV          959  TTCGAA               TT^CGAA
PflFI         660  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII         398  CAGCTG               CAG^CTG
SfuI          959  TTCGAA               TT^CGAA
SspI          850  AATATT               AAT^ATT
StuI          285  AGGCCT               AGG^CCT
TaqI          960  TCGA                 T^CGA
Tth111I       660  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       960  TCGA                 T^CGA
XhoII         556  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AciI          515  CCGC                 CCGC(-3/-1)
              571  CCGC                
AlwI          346  GATCC                GGATC(4/5)
              556  GGATC               
BsaJI         205  CCGAGG               C^C[ACGT][ACGT]GG
              759  CCCAGG              
BsrI            9  ACTGG                ACTGG(1/-1)
               82  CCAGT               
BstXI         229  CCATCTAATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              900  CCATGAGTGTGG        
SfaNI         547  GCATC                GCATC(5/9)
              829  GCATC               
SfcI          464  CTACAG               C^T[GA][CT]AG
              923  CTGTAG              


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AcsI        AluI        ApoI        BbsI        BbvI        
BpuAI       BseRI       BsiYI       BslI        BsmFI       
BssKI       BstNI       Cfr13I      DpnII       EcoNI       
EcoRII      Fnu4HI      HaeIII      HinfI       ItaI        
MboI        MboII       MnlI        MseI        MvaI        
NdeII       NlaIII      Sau3AI      Sau96I      ScrFI       
TfiI        Tru9I       TseI        Tsp509I