Restriction map for
pFN21AB7751
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BfrI
BglI BlnI BpmI BpuAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CfoI Cfr10I
ClaI CpoI DraIII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI Ksp632I KspI MamI MluI
MroI MslI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AclI 496 AACGTT AA^CGTT
AvaI 953 CCCGGG C^[CT]CG[GA]G
BalI 755 TGGCCA TGG^CCA
BanII 629 GAGCTC G[GA]GC[CT]^C
BbrPI 1005 CACGTG CAC^GTG
BclI 609 TGATCA T^GATCA
BfaI 769 CTAG C^TAG
BlpI 867 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 867 GCTAAGC GC^T[ACGT]AGC
BsaAI 1005 CACGTG [CT]AC^GT[GA]
BsaI 449 GGTCTC GGTCTC(1/5)
BsgI 907 GTGCAG GTGCAG(16/14)
BsmI 227 GCATTC GAATGC(1/-1)
BsoBI 953 CCCGGG C^[CT]CG[GA]G
Bsp1407I 354 TGTACA T^GTACA
BspMI 254 GCAGGT ACCTGC(4/8)
BsrBI 332 GAGCGG CCGCTC(-3/-3)
BsrDI 267 GCAATG GCAATG(2/0)
BsrGI 354 TGTACA T^GTACA
BstAPI 645 GCACTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 475 CCTGG CC^[AT]GG
CelII 867 GCTAAGC GC^T[ACGT]AGC
DraI 828 TTTAAA TTT^AAA
DraII 1179 AGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 124 GACAACGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 755 TGGCCA [CT]^GGCC[GA]
EcoO109I 1179 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 475 CCTGG ^CC[AT]GG
EcoT22I 1129 ATGCAT ATGCA^T
FauI 334 GCGGG CCCGC(4/6)
FbaI 609 TGATCA T^GATCA
HaeIII 756 GGCC GG^CC
HapII 954 CCGG C^CGG
HgaI 221 GACGC GACGC(5/10)
HpaII 954 CCGG C^CGG
MaeI 769 CTAG C^TAG
MluNI 755 TGGCCA TGG^CCA
MscI 755 TGGCCA TGG^CCA
MspI 954 CCGG C^CGG
MvaI 475 CCTGG CC^[AT]GG
NsiI 1129 ATGCAT ATGCA^T
PflMI 1153 CCATTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1005 CACGTG CAC^GTG
PmlI 1005 CACGTG CAC^GTG
PpuMI 1179 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 496 AACGTT AA^CGTT
PstI 994 CTGCAG CTGCA^G
SacI 629 GAGCTC GAGCT^C
SfcI 994 CTGCAG C^T[GA][CT]AG
SmaI 953 CCCGGG CCC^GGG
SspBI 354 TGTACA T^GTACA
TaqI 312 TCGA T^CGA
TthHB8I 312 TCGA T^CGA
Van91I 1153 CCATTAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 768 TCTAGA T^CTAGA
XmaCI 953 CCCGGG C^CCGGG
XmaI 953 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 723 CCGC CCGC(-3/-1)
334 GCGG
AfaI 355 GTAC GT^AC
1269 GTAC
AspHI 629 GAGCTC G[AT]GC[AT]^C
643 GAGCAC
AvaII 30 GGACC G^G[AT]CC
1180 GGTCC
BcnI 953 CCCGG CC^[GC]GG
954 CCGGG
BglII 458 AGATCT A^GATCT
1211 AGATCT
BmyI 629 GAGCTC G[AGT]GC[ACT]^C
643 GAGCAC
BsiHKAI 629 GAGCTC G[AT]GC[AT]^C
643 GAGCAC
BsmFI 158 GGGAC GGGAC(10/14)
1181 GTCCC
Bsp1286I 629 GAGCTC G[AGT]GC[ACT]^C
643 GAGCAC
Cfr13I 30 GGACC G^G[ACGT]CC
1180 GGTCC
HphI 520 GGTGA GGTGA(8/7)
720 TCACC
MaeIII 239 GTCAC ^GT[ACGT]AC
368 GTGAC
MboII 386 GAAGA GAAGA(8/7)
215 TCTTC
MfeI 836 CAATTG C^AATTG
1330 CAATTG
MspA1I 192 CAGCTG C[AC]G^C[GT]G
729 CAGCTG
MunI 836 CAATTG C^AATTG
1330 CAATTG
NciI 953 CCCGG CC^[GC]GG
954 CCGGG
PleI 949 GACTC GAGTC(4/5)
237 GAGTC
PvuII 192 CAGCTG CAG^CTG
729 CAGCTG
RsaI 355 GTAC GT^AC
1269 GTAC
Sau96I 30 GGACC G^G[ACGT]CC
1180 GGTCC
SmlI 468 CTCAAG C^T[CT][GA]AG
1145 CTCAAG
Tsp45I 239 GTCAC ^GT[GC]AC
368 GTGAC
XcmI 419 CCATTAACACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1184 CCATGTCAACACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstYI DdeI DpnII Fnu4HI FokI
HincII HindII HinfI ItaI MaeII
MboI MflI MnlI MseI MwoI
NdeII NlaIII NlaIV Sau3AI ScrFI
SfaNI TfiI Tru9I TseI Tsp509I
TspRI XhoII