##################################
# NO CUT ENZYMES (  212 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BanI        
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsiYI       BslI        
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      Cac8I       CelII       
Cfr10I      Cfr13I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT22I     FauI        FbaI        
Fnu4HI      FseI        FspI        HaeII       HaeIII      
HapII       HgaI        Hin1I       HindIII     HpaII       
HphI        ItaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacII       SalI        SapI        Sau96I      
SbfI        ScaI        ScrFI       SexAI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
TseI        Tsp45I      TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   23 enzymes) #
###################################

AcsI          373  AAATTT               [GA]^AATT[CT]
ApoI          373  AAATTT               [GA]^AATT[CT]
AseI          136  ATTAAT               AT^TAAT
AsnI          136  ATTAAT               AT^TAAT
AspHI         317  GAGCTC               G[AT]GC[AT]^C
BamHI         166  GGATCC               G^GATCC
BsiHKAI       317  GAGCTC               G[AT]GC[AT]^C
BsmAI         331  GAGAC                GTCTC(1/5)
CfoI          267  GCGC                 GCG^C
DraI          210  TTTAAA               TTT^AAA
EcoT14I       199  CCAAGG               C^C[AT][AT]GG
HhaI          267  GCGC                 GCG^C
HinP1I        267  GCGC                 G^CGC
HinfI         255  GAATC                G^A[ACGT]TC
HpaI          142  GTTAAC               GTT^AAC
MaeIII          3  GTTAC                ^GT[ACGT]AC
MnlI          287  GAGG                 CCTC(7/6)
NlaIII        252  CATG                 CATG^
PshBI         136  ATTAAT               AT^TAAT
SacI          317  GAGCTC               GAGCT^C
SfaNI          85  GATGC                GCATC(5/9)
StyI          199  CCAAGG               C^C[AT][AT]GG
TfiI          255  GAATC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BanII         317  GAGCTC               G[GA]GC[CT]^C
              420  GAGCCC              
BmyI          317  GAGCTC               G[AGT]GC[ACT]^C
              420  GAGCCC              
BsaJI         199  CCAAGG               C^C[ACGT][ACGT]GG
              285  CCGAGG              
Bsp1286I      317  GAGCTC               G[AGT]GC[ACT]^C
              420  GAGCCC              
BstF5I         80  CATCC                GGATG(2/0)
              456  CATCC               
BstYI         166  GGATCC               [GA]^GATC[CT]
              289  GGATCT              
DdeI           53  CTCAG                C^T[ACGT]AG
              380  CTAAG               
DpnII         167  GATC                 ^GATC
              290  GATC                
FokI           80  CATCC                GGATG(9/13)
              456  CATCC               
HincII        142  GTTAAC               GT[CT]^[GA]AC
              244  GTCAAC              
HindII        142  GTTAAC               GT[CT]^[GA]AC
              244  GTCAAC              
MboI          167  GATC                 ^GATC
              290  GATC                
MflI          166  GGATCC               [GA]^GATC[CT]
              289  GGATCT              
NdeII         167  GATC                 ^GATC
              290  GATC                
NlaIV         166  GGATCC               GG[ACGT]^[ACGT]CC
              419  GGAGCC              
Sau3AI        167  GATC                 ^GATC
              290  GATC                
XhoII         166  GGATCC               [GA]^GATC[CT]
              289  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

AluI        AlwI        MboII       MseI        Tru9I       
Tsp509I