Restriction map for
pFN21AB7309
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# NO CUT ENZYMES ( 92 enzymes) #
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AatII Acc65I AclI AcyI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AvrII BanI
BbeI BbvCI BclI BfrI BlnI
BlpI Bpu1102I BsaHI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FbaI
FseI Hin1I HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
SacII SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 45 GTCGAC GT^[AC][GT]AC
AccIII 83 TCCGGA T^CCGGA
ApaI 1941 GGGCCC GGGCC^C
AspI 849 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 18 CCCGGG C^[CT]CG[GA]G
AviII 917 TGCGCA TGC^GCA
BamHI 929 GGATCC G^GATCC
BcgI 953 CGACAGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1100 GTATCC GTATCC(6/5)
BsaI 2525 GAGACC GGTCTC(1/5)
BseAI 83 TCCGGA T^CCGGA
BsmI 948 GCATTC GAATGC(1/-1)
BsoBI 18 CCCGGG C^[CT]CG[GA]G
BspEI 83 TCCGGA T^CCGGA
BsrBI 1321 GAGCGG CCGCTC(-3/-3)
BsrDI 621 CATTGC GCAATG(2/0)
BsrFI 1469 GCCGGT [GA]^CCGG[CT]
BstAPI 89 GCAAAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1469 GCCGGT [GA]^CCGG[CT]
CpoI 310 CGGACCG CG^G[AT]CCG
DraI 297 TTTAAA TTT^AAA
DrdI 36 GACTTCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2338 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 419 GATATC GAT^ATC
FspI 917 TGCGCA TGC^GCA
HaeII 2322 AGCGCC [GA]GCGC^[CT]
HgaI 174 GACGC GACGC(5/10)
HindIII 187 AAGCTT A^AGCTT
MroI 83 TCCGGA T^CCGGA
NspI 94 ACATGC [GA]CATG^[CT]
PflFI 849 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 310 CGGACCG CG^G[AT]CCG
SalI 45 GTCGAC G^TCGAC
SmaI 18 CCCGGG CCC^GGG
SmlI 1318 CTTGAG C^T[CT][GA]AG
StuI 957 AGGCCT AGG^CCT
Tth111I 849 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 170 CCAGGACGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 18 CCCGGG C^CCGGG
XmaI 18 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 176 CGCG CG^CG
892 CGCG
Alw44I 344 GTGCAC G^TGCAC
2174 GTGCAC
AocI 924 CCTCAGG CC^T[ACGT]AGG
981 CCTTAGG
ApaLI 344 GTGCAC G^TGCAC
2174 GTGCAC
Asp700I 777 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2373 GAAACTGTTC
BbrPI 703 CACGTG CAC^GTG
2717 CACGTG
BglI 1483 GCCAGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1689 GCCTGAGAGGC
BglII 389 AGATCT A^GATCT
902 AGATCT
BsaAI 703 CACGTG [CT]AC^GT[GA]
2717 CACGTG
BsaBI 268 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
752 GATTCTTATC
BsaWI 83 TCCGGA [AT]^CCGG[AT]
308 ACCGGA
BssSI 208 CACGAG CACGAG(-5/-1)
1561 CACGAG
BstUI 176 CGCG CG^CG
892 CGCG
BstXI 766 CCAAATAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1465 CCAAGCCGGTGG
Bsu36I 924 CCTCAGG CC^T[ACGT]AGG
981 CCTTAGG
Eco81I 924 CCTCAGG CC^T[ACGT]AGG
981 CCTTAGG
EcoT14I 734 CCATGG C^C[AT][AT]GG
1028 CCATGG
HincII 45 GTCGAC GT[CT]^[GA]AC
2014 GTTGAC
HindII 45 GTCGAC GT[CT]^[GA]AC
2014 GTTGAC
MamI 268 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
752 GATTCTTATC
MvnI 176 CGCG CG^CG
892 CGCG
NcoI 734 CCATGG C^CATGG
1028 CCATGG
NdeI 325 CATATG CA^TATG
1706 CATATG
PflMI 324 CCATATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1611 CCAGAAACTGG
PmaCI 703 CACGTG CAC^GTG
2717 CACGTG
PmlI 703 CACGTG CAC^GTG
2717 CACGTG
PpuMI 1974 AGGACCT [GA]G^G[AT]CC[CT]
2334 GGGACCT
SacI 2103 GAGCTC GAGCT^C
2449 GAGCTC
StyI 734 CCATGG C^C[AT][AT]GG
1028 CCATGG
TfiI 752 GATTC G^A[AT]TC
911 GAATC
Van91I 324 CCATATGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1611 CCAGAAACTGG
XmnI 777 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2373 GAAACTGTTC
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PshAI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII