Restriction map for
pFN21AE1683
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BbsI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BseAI BsgI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstZ17I Bsu36I
CelII ClaI DraI DraII DraIII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI Ksp632I MaeII MaeIII MamI
MfeI MluI MroI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI SalI SapI
SbfI SexAI SfaNI SfiI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp45I Tsp509I TspRI Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcyI 108 GGCGCC G[GA]^CG[CT]C
AfaI 213 GTAC GT^AC
AlwI 483 GGATC GGATC(4/5)
AspHI 98 GAGCTC G[AT]GC[AT]^C
AvaI 93 CCCGGG C^[CT]CG[GA]G
BalI 521 TGGCCA TGG^CCA
BbeI 108 GGCGCC GGCGC^C
BbvCI 121 CCTCAGC CCTCAGC(-5/-2)
BfaI 410 CTAG C^TAG
BsaHI 108 GGCGCC G[GA]^CG[CT]C
BsaWI 441 ACCGGA [AT]^CCGG[AT]
BsiHKAI 98 GAGCTC G[AT]GC[AT]^C
BsmFI 91 GTCCC GGGAC(10/14)
BsmI 414 GAATGC GAATGC(1/-1)
BsoBI 93 CCCGGG C^[CT]CG[GA]G
BsrBI 251 CCGCTC CCGCTC(-3/-3)
BstXI 473 CCATCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 483 GGATCT [GA]^GATC[CT]
CpoI 457 CGGTCCG CG^G[AT]CCG
DpnII 484 GATC ^GATC
EcoT14I 128 CCTTGG C^C[AT][AT]GG
Hin1I 108 GGCGCC G[GA]^CG[CT]C
HinfI 29 GAGTC G^A[ACGT]TC
HphI 488 TCACC GGTGA(8/7)
KasI 108 GGCGCC G^GCGCC
MaeI 410 CTAG C^TAG
MboI 484 GATC ^GATC
MboII 137 TCTTC GAAGA(8/7)
MflI 483 GGATCT [GA]^GATC[CT]
MluNI 521 TGGCCA TGG^CCA
MscI 521 TGGCCA TGG^CCA
NaeI 283 GCCGGC GCC^GGC
NarI 108 GGCGCC GG^CGCC
NdeII 484 GATC ^GATC
NgoMIV 283 GCCGGC G^CCGGC
PleI 29 GAGTC GAGTC(4/5)
PstI 346 CTGCAG CTGCA^G
PvuII 352 CAGCTG CAG^CTG
RsaI 213 GTAC GT^AC
RsrII 457 CGGTCCG CG^G[AT]CCG
SacI 98 GAGCTC GAGCT^C
Sau3AI 484 GATC ^GATC
ScaI 212 AGTACT AGT^ACT
SfcI 346 CTGCAG C^T[GA][CT]AG
SfoI 108 GGCGCC GGC^GCC
SgrAI 282 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 93 CCCGGG CCC^GGG
StyI 128 CCTTGG C^C[AT][AT]GG
TaqI 377 TCGA T^CGA
TthHB8I 377 TCGA T^CGA
XhoII 483 GGATCT [GA]^GATC[CT]
XmaCI 93 CCCGGG C^CCGGG
XmaI 93 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AlwNI 349 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
352 CAGCTGCTG
BanI 108 GGCGCC G^G[CT][GA]CC
438 GGCACC
BanII 98 GAGCTC G[GA]GC[CT]^C
243 GAGCCC
BmyI 98 GAGCTC G[AGT]GC[ACT]^C
243 GAGCCC
Bsp1286I 98 GAGCTC G[AGT]GC[ACT]^C
243 GAGCCC
BstAPI 348 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
351 GCAGCTGCTGC
DdeI 76 CTGAG C^T[ACGT]AG
122 CTCAG
EagI 184 CGGCCG C^GGCCG
248 CGGCCG
EclXI 184 CGGCCG C^GGCCG
248 CGGCCG
Eco52I 184 CGGCCG C^GGCCG
248 CGGCCG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AvaII BbvI
BcnI BsaJI BseRI BsiEI BsiYI
BslI BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DsaI
EaeI EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI KspI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV SacII Sau96I ScrFI TseI