Restriction map for
pFN21AE6262
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BalI BbrPI
BbsI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrFI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FseI FspI HincII
HindII HpaI KpnI Ksp632I KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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ApaI 101 GGGCCC GGGCC^C
Asp700I 658 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 321 GGATCC G^GATCC
BanI 95 GGCGCC G^G[CT][GA]CC
BanII 101 GGGCCC G[GA]GC[CT]^C
BbeI 95 GGCGCC GGCGC^C
BclI 626 TGATCA T^GATCA
BfaI 266 CTAG C^TAG
BglII 255 AGATCT A^GATCT
BmyI 101 GGGCCC G[AGT]GC[ACT]^C
BsaBI 178 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 51 ACCGGA [AT]^CCGG[AT]
BsgI 251 GTGCAG GTGCAG(16/14)
BsmI 827 GCATTC GAATGC(1/-1)
Bsp1286I 101 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 429 TGTACA T^GTACA
BsrGI 429 TGTACA T^GTACA
BssHII 168 GCGCGC G^CGCGC
DraII 365 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 367 GACCCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 282 CCTCCTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 365 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 745 GAATTC G^AATTC
EcoT22I 670 ATGCAT ATGCA^T
FbaI 626 TGATCA T^GATCA
HgaI 116 GCGTC GACGC(5/10)
HindIII 693 AAGCTT A^AGCTT
KasI 95 GGCGCC G^GCGCC
MaeI 266 CTAG C^TAG
MaeII 392 ACGT A^CGT
MaeIII 204 GTTAC ^GT[ACGT]AC
MamI 178 GATTTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 653 TTAA T^TAA
MslI 667 CACATGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 95 GGCGCC GG^CGCC
NsiI 670 ATGCAT ATGCA^T
NspI 668 ACATGC [GA]CATG^[CT]
PpuMI 365 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 760 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 70 CTGCAG CTGCA^G
SfaNI 578 GCATC GCATC(5/9)
SfcI 70 CTGCAG C^T[GA][CT]AG
SfoI 95 GGCGCC GGC^GCC
SspBI 429 TGTACA T^GTACA
Tru9I 653 TTAA T^TAA
XmnI 658 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 745 GAATTC [GA]^AATT[CT]
780 AAATTT
AcyI 95 GGCGCC G[GA]^CG[CT]C
115 GGCGTC
AfaI 430 GTAC GT^AC
544 GTAC
ApoI 745 GAATTC [GA]^AATT[CT]
780 AAATTT
AvaI 104 CCCGGG C^[CT]CG[GA]G
173 CCCGGG
BsaHI 95 GGCGCC G[GA]^CG[CT]C
115 GGCGTC
BsmFI 365 GGGAC GGGAC(10/14)
118 GTCCC
BsoBI 104 CCCGGG C^[CT]CG[GA]G
173 CCCGGG
BsrBI 121 CCGCTC CCGCTC(-3/-3)
136 CCGCTC
DsaI 300 CCATGG C^C[GA][CT]GG
752 CCATGG
EcoT14I 300 CCATGG C^C[AT][AT]GG
752 CCATGG
FauI 120 CCCGC CCCGC(4/6)
28 GCGGG
HaeII 87 GGCGCT [GA]GCGC^[CT]
95 GGCGCC
Hin1I 95 GGCGCC G[GA]^CG[CT]C
115 GGCGTC
HphI 739 GGTGA GGTGA(8/7)
140 TCACC
MspA1I 130 CCGCTG C[AC]G^C[GT]G
448 CAGCGG
NcoI 300 CCATGG C^CATGG
752 CCATGG
PleI 145 GACTC GAGTC(4/5)
305 GAGTC
RsaI 430 GTAC GT^AC
544 GTAC
SmaI 104 CCCGGG CCC^GGG
173 CCCGGG
StyI 300 CCATGG C^C[AT][AT]GG
752 CCATGG
TaqI 182 TCGA T^CGA
399 TCGA
TspRI 327 CACTG CA[GC]TG[ACGT][ACGT]^
361 CACTG
TthHB8I 182 TCGA T^CGA
399 TCGA
XmaCI 104 CCCGGG C^CCGGG
173 CCCGGG
XmaI 104 CCCGGG C^CCGGG
173 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BbvI BcnI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MboII MflI MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TfiI TseI Tsp509I XhoII