Restriction map for
pFN21AE9511
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmAI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
Bst1107I BstBI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FspI HindIII
HpaI KpnI KspI MamI MfeI
MflI MroI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacII SbfI ScaI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AlwI 491 GGATC GGATC(4/5)
ApaI 45 GGGCCC GGGCC^C
AvaI 583 CCCGAG C^[CT]CG[GA]G
AvaII 51 GGACC G^G[AT]CC
BcgI 326 GCAAGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 60 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 60 GCTGAGC GC^T[ACGT]AGC
BsaAI 178 TACGTG [CT]AC^GT[GA]
BseRI 781 CTCCTC GAGGAG(10/8)
BsoBI 583 CCCGAG C^[CT]CG[GA]G
BspHI 386 TCATGA T^CATGA
BssSI 622 CTCGTG CACGAG(-5/-1)
BstAPI 690 GCATGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 744 GGTGACC G^GT[ACGT]ACC
BstPI 744 GGTGACC G^GT[ACGT]ACC
CelII 60 GCTGAGC GC^T[ACGT]AGC
DraII 719 AGGCCCT [GA]G^G[ACGT]CC[CT]
EagI 444 CGGCCG C^GGCCG
EarI 253 CTCTTC CTCTTC(1/4)
EclXI 444 CGGCCG C^GGCCG
Eco52I 444 CGGCCG C^GGCCG
EcoO109I 719 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 744 GGTGACC G^GT[ACGT]ACC
EcoT14I 716 CCAAGG C^C[AT][AT]GG
FseI 441 GGCCGGCC GGCCGG^CC
HincII 78 GTCGAC GT[CT]^[GA]AC
HindII 78 GTCGAC GT[CT]^[GA]AC
Ksp632I 253 CTCTTC CTCTTC(1/4)
MaeII 179 ACGT A^CGT
MluI 74 ACGCGT A^CGCGT
NspI 690 GCATGC [GA]CATG^[CT]
RcaI 386 TCATGA T^CATGA
SalI 78 GTCGAC G^TCGAC
SapI 252 GCTCTTC GCTCTTC(1/4)
SexAI 404 ACCTGGT A^CC[AT]GGT
SfaNI 40 GCATC GCATC(5/9)
SfcI 453 CTACAG C^T[GA][CT]AG
SphI 690 GCATGC GCATG^C
StuI 587 AGGCCT AGG^CCT
StyI 716 CCAAGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 78 GTCGAC GT^[AC][GT]AC
127 GTCTAC
AflIII 74 ACGCGT A^C[GA][CT]GT
179 ACGTGT
BalI 236 TGGCCA TGG^CCA
416 TGGCCA
BbsI 204 GAAGAC GAAGAC(2/6)
523 GTCTTC
BfaI 535 CTAG C^TAG
568 CTAG
BpmI 363 CTCCAG CTGGAG(16/14)
724 CTGGAG
BpuAI 204 GAAGAC GAAGAC(2/6)
523 GTCTTC
BsiEI 396 CGACCG CG[GA][CT]^CG
444 CGGCCG
BstF5I 225 CATCC GGATG(2/0)
639 CATCC
BstXI 695 CCATGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
716 CCAAGGCCCTGG
DpnII 138 GATC ^GATC
492 GATC
DsaI 146 CCGTGG C^C[GA][CT]GG
812 CCGTGG
FokI 225 CATCC GGATG(9/13)
639 CATCC
HinfI 81 GACTC G^A[ACGT]TC
427 GAGTC
MaeI 535 CTAG C^TAG
568 CTAG
MboI 138 GATC ^GATC
492 GATC
MluNI 236 TGGCCA TGG^CCA
416 TGGCCA
MscI 236 TGGCCA TGG^CCA
416 TGGCCA
NdeII 138 GATC ^GATC
492 GATC
PleI 81 GACTC GAGTC(4/5)
427 GAGTC
PvuII 897 CAGCTG CAG^CTG
958 CAGCTG
SacI 250 GAGCTC GAGCT^C
316 GAGCTC
Sau3AI 138 GATC ^GATC
492 GATC
TaqI 79 TCGA T^CGA
344 TCGA
TthHB8I 79 TCGA T^CGA
344 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI BanI BanII BbeI BbvI
BcnI BmyI BsaHI BsaJI BsiHKAI
BsiYI BslI Bsp1286I BsrFI BsrI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI EaeI
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI MaeIII
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NgoMIV NlaIII NlaIV RsaI
Sau96I ScrFI SfoI TseI Tsp45I
TspRI