Restriction map for
pFN21AE2132
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# NO CUT ENZYMES ( 71 enzymes) #
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AatII AclI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AspEI
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BglII BlnI
BsaI BspHI BspLU11I BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
FseI HpaI KasI MfeI MluI
MluNI MscI MunI NarI NcoI
NdeI NheI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RcaI RsrII
SapI ScaI SfiI SfoI SfuI
SgrAI SpeI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 1537 GGTACC G^GTACC
AccIII 1071 TCCGGA T^CCGGA
AflII 1345 CTTAAG C^TTAAG
AocI 1957 CCTCAGG CC^T[ACGT]AGG
Asp718I 1537 GGTACC G^GTACC
AviII 189 TGCGCA TGC^GCA
BanI 1537 GGTACC G^G[CT][GA]CC
BfrI 1345 CTTAAG C^TTAAG
BlpI 2216 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2216 GCTTAGC GC^T[ACGT]AGC
BsaBI 1509 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1071 TCCGGA [AT]^CCGG[AT]
BseAI 1071 TCCGGA T^CCGGA
BsiEI 957 CGGCCG CG[GA][CT]^CG
BsiWI 1634 CGTACG C^GTACG
BsmBI 105 GAGACG CGTCTC(1/5)
BsmI 225 GCATTC GAATGC(1/-1)
BspDI 1717 ATCGAT AT^CGAT
BspEI 1071 TCCGGA T^CCGGA
BspMI 173 ACCTGC ACCTGC(4/8)
BsrBI 1026 CCGCTC CCGCTC(-3/-3)
BsrFI 38 GCCGGC [GA]^CCGG[CT]
BssHII 1825 GCGCGC G^CGCGC
BstXI 2264 CCATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 381 GGATCT [GA]^GATC[CT]
Bsu36I 1957 CCTCAGG CC^T[ACGT]AGG
CelII 2216 GCTTAGC GC^T[ACGT]AGC
Cfr10I 38 GCCGGC [GA]^CCGG[CT]
ClaI 1717 ATCGAT AT^CGAT
DraI 621 TTTAAA TTT^AAA
DraII 368 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 2528 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 712 GACATGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 957 CGGCCG [CT]^GGCC[GA]
EagI 957 CGGCCG C^GGCCG
EclXI 957 CGGCCG C^GGCCG
Eco52I 957 CGGCCG C^GGCCG
Eco81I 1957 CCTCAGG CC^T[ACGT]AGG
EcoNI 1970 CCTATGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 368 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1394 CCTTGG C^C[AT][AT]GG
EcoT22I 223 ATGCAT ATGCA^T
FspI 189 TGCGCA TGC^GCA
HaeII 986 GGCGCT [GA]GCGC^[CT]
KpnI 1537 GGTACC GGTAC^C
MamI 1509 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 381 GGATCT [GA]^GATC[CT]
MroI 1071 TCCGGA T^CCGGA
NaeI 38 GCCGGC GCC^GGC
NgoMIV 38 GCCGGC G^CCGGC
NotI 956 GCGGCCGC GC^GGCCGC
NsiI 223 ATGCAT ATGCA^T
PshAI 1975 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2038 GAGCTC GAGCT^C
SalI 15 GTCGAC G^TCGAC
SbfI 1084 CCTGCAGG CCTGCA^GG
SexAI 1994 ACCTGGT A^CC[AT]GGT
SmaI 938 CCCGGG CCC^GGG
SmlI 1345 CTTAAG C^T[CT][GA]AG
SnaBI 1636 TACGTA TAC^GTA
SphI 481 GCATGC GCATG^C
Sse8387I 1084 CCTGCAGG CCTGCA^GG
StyI 1394 CCTTGG C^C[AT][AT]GG
XhoII 381 GGATCT [GA]^GATC[CT]
XmaCI 938 CCCGGG C^CCGGG
XmaI 938 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AseI 598 ATTAAT AT^TAAT
2208 ATTAAT
AsnI 598 ATTAAT AT^TAAT
2208 ATTAAT
AspHI 1682 GTGCTC G[AT]GC[AT]^C
2038 GAGCTC
AspI 1138 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1782 GACTCTGTC
BclI 866 TGATCA T^GATCA
1052 TGATCA
BfaI 436 CTAG C^TAG
2200 CTAG
BpmI 1611 CTCCAG CTGGAG(16/14)
164 CTGGAG
BsaAI 1564 TACGTG [CT]AC^GT[GA]
1636 TACGTA
BsgI 359 CTGCAC GTGCAG(16/14)
1616 GTGCAG
BsiHKAI 1682 GTGCTC G[AT]GC[AT]^C
2038 GAGCTC
Bsp1407I 665 TGTACA T^GTACA
1568 TGTACA
BsrGI 665 TGTACA T^GTACA
1568 TGTACA
EarI 1033 CTCTTC CTCTTC(1/4)
2275 GAAGAG
FbaI 866 TGATCA T^GATCA
1052 TGATCA
HincII 15 GTCGAC GT[CT]^[GA]AC
1135 GTTGAC
HindII 15 GTCGAC GT[CT]^[GA]AC
1135 GTTGAC
HindIII 1057 AAGCTT A^AGCTT
2419 AAGCTT
Ksp632I 1033 CTCTTC CTCTTC(1/4)
2275 GAAGAG
MaeI 436 CTAG C^TAG
2200 CTAG
PflFI 1138 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1782 GACTCTGTC
PflMI 307 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1002 CCACAGCATGG
PshBI 598 ATTAAT AT^TAAT
2208 ATTAAT
SspBI 665 TGTACA T^GTACA
1568 TGTACA
StuI 343 AGGCCT AGG^CCT
902 AGGCCT
Tth111I 1138 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1782 GACTCTGTC
Van91I 307 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1002 CCACAGCATGG
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AvaI AvaII BanII
BbsI BbvI BcnI BglI BmyI
BpuAI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KspI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XmnI