Restriction map for
pFN21AE4039
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglI
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BseRI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsoBI Bsp1286I BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRV EcoT14I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspI StuI StyI
SwaI Tsp45I Tth111I Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 386 GTAGAC GT^[AC][GT]AC
AcsI 677 GAATTC [GA]^AATT[CT]
AflIII 251 ACATGT A^C[GA][CT]GT
AlwI 525 GGATC GGATC(4/5)
ApoI 677 GAATTC [GA]^AATT[CT]
AseI 466 ATTAAT AT^TAAT
AsnI 466 ATTAAT AT^TAAT
Asp700I 193 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2 TGGCCA TGG^CCA
BanI 322 GGTGCC G^G[CT][GA]CC
BbvI 223 GCTGC GCAGC(8/12)
BclI 429 TGATCA T^GATCA
BcnI 288 CCCGG CC^[GC]GG
BglII 502 AGATCT A^GATCT
BsaI 295 GAGACC GGTCTC(1/5)
BsaJI 116 CCCTGG C^C[ACGT][ACGT]GG
BsaWI 479 ACCGGA [AT]^CCGG[AT]
BsgI 271 CTGCAC GTGCAG(16/14)
BsmFI 260 GTCCC GGGAC(10/14)
BsmI 550 GCATTC GAATGC(1/-1)
Bsp1407I 141 TGTACA T^GTACA
BspLU11I 251 ACATGT A^CATGT
BspMI 219 ACCTGC ACCTGC(4/8)
BsrGI 141 TGTACA T^GTACA
BstAPI 217 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 396 GGTAACC G^GT[ACGT]ACC
BstPI 396 GGTAACC G^GT[ACGT]ACC
Cfr13I 576 GGGCC G^G[ACGT]CC
DraII 575 GGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoO109I 575 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 396 GGTAACC G^GT[ACGT]ACC
EcoRI 677 GAATTC G^AATTC
EcoT22I 548 ATGCAT ATGCA^T
FbaI 429 TGATCA T^GATCA
Fnu4HI 223 GCTGC GC^[ACGT]GC
ItaI 223 GCTGC GC^[ACGT]GC
MaeIII 397 GTAAC ^GT[ACGT]AC
MboII 424 GAAGA GAAGA(8/7)
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 246 CACTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 288 CCCGG CC^[GC]GG
NsiI 548 ATGCAT ATGCA^T
PshBI 466 ATTAAT AT^TAAT
Sau96I 576 GGGCC G^G[ACGT]CC
ScaI 75 AGTACT AGT^ACT
SfcI 384 CTGTAG C^T[GA][CT]AG
SphI 546 GCATGC GCATG^C
SspBI 141 TGTACA T^GTACA
TseI 223 GCTGC G^C[AT]GC
TspRI 246 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 500 CCAGATCTATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 193 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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BsmAI 295 GAGAC GTCTC(1/5)
435 GTCTC
BsrI 36 ACTGG ACTGG(1/-1)
341 ACTGG
BstNI 117 CCTGG CC^[AT]GG
168 CCAGG
BstYI 502 AGATCT [GA]^GATC[CT]
525 GGATCT
EcoRII 117 CCTGG ^CC[AT]GG
168 CCAGG
HaeIII 3 GGCC GG^CC
577 GGCC
HapII 289 CCGG C^CGG
480 CCGG
HinfI 46 GATTC G^A[ACGT]TC
485 GATTC
HpaII 289 CCGG C^CGG
480 CCGG
MflI 502 AGATCT [GA]^GATC[CT]
525 GGATCT
MseI 467 TTAA T^TAA
616 TTAA
MspI 289 CCGG C^CGG
480 CCGG
MvaI 117 CCTGG CC^[AT]GG
168 CCAGG
MwoI 103 GCTTTGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
217 GCACCTGCTGC
NlaIV 322 GGTGCC GG[ACGT]^[ACGT]CC
575 GGGGCC
NspI 251 ACATGT [GA]CATG^[CT]
546 GCATGC
TfiI 46 GATTC G^A[AT]TC
485 GATTC
Tru9I 467 TTAA T^TAA
616 TTAA
Tsp509I 519 AATT ^AATT
678 AATT
XbaI 648 TCTAGA T^CTAGA
690 TCTAGA
XhoII 502 AGATCT [GA]^GATC[CT]
525 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AfaI AluI BfaI BssKI BstF5I
Cac8I DdeI DpnII FokI HphI
MaeI MboI MnlI NdeII NlaIII
RsaI Sau3AI ScrFI SfaNI TaqI
TthHB8I