Restriction map for
pFN21AE4475
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AvaI AviII
AvrII BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoT22I FauI
FseI FspI HaeII HapII Hin1I
HincII HindII HpaI HpaII KasI
KpnI KspI MaeI MamI MfeI
MluI MroI MspI MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 325 CGCG CG^CG
AciI 987 CCGC CCGC(-3/-1)
AclI 160 AACGTT AA^CGTT
AseI 733 ATTAAT AT^TAAT
AsnI 733 ATTAAT AT^TAAT
AspHI 1270 GAGCAC G[AT]GC[AT]^C
AspI 169 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 291 GGATCC G^GATCC
BbsI 1023 GAAGAC GAAGAC(2/6)
BpuAI 1023 GAAGAC GAAGAC(2/6)
BsaAI 1372 TACGTG [CT]AC^GT[GA]
BseRI 100 GAGGAG GAGGAG(10/8)
BsiHKAI 1270 GAGCAC G[AT]GC[AT]^C
BsmFI 898 GTCCC GGGAC(10/14)
BsmI 1180 GAATGC GAATGC(1/-1)
BspMI 917 ACCTGC ACCTGC(4/8)
BstAPI 1002 GCACACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 325 CGCG CG^CG
BstYI 291 GGATCC [GA]^GATC[CT]
CfoI 285 GCGC GCG^C
DdeI 98 CTGAG C^T[ACGT]AG
DraI 73 TTTAAA TTT^AAA
DraII 1052 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoNI 1132 CCTGCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1052 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 481 GATATC GAT^ATC
HhaI 285 GCGC GCG^C
HinP1I 285 GCGC G^CGC
HindIII 1144 AAGCTT A^AGCTT
MflI 291 GGATCC [GA]^GATC[CT]
MspA1I 205 CAGCTG C[AC]G^C[GT]G
MvnI 325 CGCG CG^CG
PflFI 169 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1052 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 733 ATTAAT AT^TAAT
Psp1406I 160 AACGTT AA^CGTT
PvuII 205 CAGCTG CAG^CTG
TfiI 23 GATTC G^A[AT]TC
Tth111I 169 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 291 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 523 AAATTT [GA]^AATT[CT]
1263 AAATTC
AfaI 181 GTAC GT^AC
915 GTAC
AlwNI 1051 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1094 CAGGCTCTG
ApoI 523 AAATTT [GA]^AATT[CT]
1263 AAATTC
AvaII 897 GGTCC G^G[AT]CC
1053 GGTCC
BclI 501 TGATCA T^GATCA
740 TGATCA
Cfr13I 897 GGTCC G^G[ACGT]CC
1053 GGTCC
DsaI 1155 CCATGG C^C[GA][CT]GG
1379 CCATGG
FbaI 501 TGATCA T^GATCA
740 TGATCA
HgaI 816 GACGC GACGC(5/10)
258 GCGTC
MwoI 80 GCTGTGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1002 GCACACAATGC
NcoI 1155 CCATGG C^CATGG
1379 CCATGG
PleI 318 GACTC GAGTC(4/5)
444 GACTC
PstI 332 CTGCAG CTGCA^G
1436 CTGCAG
RsaI 181 GTAC GT^AC
915 GTAC
Sau96I 897 GGTCC G^G[ACGT]CC
1053 GGTCC
SfaNI 628 GATGC GCATC(5/9)
1102 GATGC
XcmI 197 CCATCACCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
884 CCACATCTCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AluI AlwI BalI BbvI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I DpnII EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MvaI NdeII NlaIII
NlaIV PflMI SapI Sau3AI ScrFI
SfcI StuI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I