Restriction map for
pFN21AE4441
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
Acc65I AccII AccIII AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AvaI AviII
AvrII BalI BanII BbeI BbrPI
BciVI BcnI BfrI BglI BlnI
BlpI BmyI BpmI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HapII HhaI HinP1I HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshBI PvuI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TspRI XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 466 GACGTC GACGT^C
AccI 568 GTCGAC GT^[AC][GT]AC
AclI 955 AACGTT AA^CGTT
AcsI 1258 AAATTT [GA]^AATT[CT]
AflIII 1212 ACATGT A^C[GA][CT]GT
ApoI 1258 AAATTT [GA]^AATT[CT]
Asp700I 1153 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 921 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 532 GGCACC G^G[CT][GA]CC
BbsI 864 GTCTTC GAAGAC(2/6)
BbvCI 47 CCTCAGC CCTCAGC(-5/-2)
BbvI 1183 GCTGC GCAGC(8/12)
BclI 554 TGATCA T^GATCA
BfaI 1162 CTAG C^TAG
BglII 967 AGATCT A^GATCT
BpuAI 864 GTCTTC GAAGAC(2/6)
BsgI 456 CTGCAC GTGCAG(16/14)
BsmAI 687 GAGAC GTCTC(1/5)
BsmBI 687 GAGACG CGTCTC(1/5)
BsmFI 641 GGGAC GGGAC(10/14)
BsmI 612 GAATGC GAATGC(1/-1)
BspHI 908 TCATGA T^CATGA
BspLU11I 1212 ACATGT A^CATGT
BspMI 896 ACCTGC ACCTGC(4/8)
BstBI 373 TTCGAA TT^CGAA
EaeI 366 CGGCCG [CT]^GGCC[GA]
EagI 366 CGGCCG C^GGCCG
EclXI 366 CGGCCG C^GGCCG
Eco52I 366 CGGCCG C^GGCCG
FauI 436 CCCGC CCCGC(4/6)
FbaI 554 TGATCA T^GATCA
HgaI 689 GACGC GACGC(5/10)
HphI 960 TCACC GGTGA(8/7)
MaeI 1162 CTAG C^TAG
MslI 145 CATTCTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 217 CAGCTG C[AC]G^C[GT]G
NspI 1212 ACATGT [GA]CATG^[CT]
NspV 373 TTCGAA TT^CGAA
PflFI 921 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1089 GAGTC GAGTC(4/5)
PshAI 390 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 955 AACGTT AA^CGTT
PstI 1018 CTGCAG CTGCA^G
PvuII 217 CAGCTG CAG^CTG
RcaI 908 TCATGA T^CATGA
SalI 568 GTCGAC G^TCGAC
SfcI 1018 CTGCAG C^T[GA][CT]AG
SfuI 373 TTCGAA TT^CGAA
SmlI 1268 CTCAAG C^T[CT][GA]AG
TseI 1183 GCTGC G^C[AT]GC
Tth111I 921 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 803 CCACATCGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1153 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 466 GACGTC G[GA]^CG[CT]C
689 GACGCC
AvaII 129 GGTCC G^G[AT]CC
428 GGACC
BamHI 296 GGATCC G^GATCC
514 GGATCC
BcgI 490 CGACAGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1109 GCAAAGTCCTCG
BsaAI 669 CACGTA [CT]AC^GT[GA]
978 CACGTA
BsaHI 466 GACGTC G[GA]^CG[CT]C
689 GACGCC
BseRI 94 CTCCTC GAGGAG(10/8)
1084 GAGGAG
BsiEI 366 CGGCCG CG[GA][CT]^CG
486 CGACCG
BsrI 1253 ACTGG ACTGG(1/-1)
1096 CCAGT
Cac8I 439 GCCAGC GC[ACGT]^[ACGT]GC
692 GCCTGC
Fnu4HI 368 GCCGC GC^[ACGT]GC
1183 GCTGC
HaeIII 367 GGCC GG^CC
993 GGCC
Hin1I 466 GACGTC G[GA]^CG[CT]C
689 GACGCC
ItaI 368 GCCGC GC^[ACGT]GC
1183 GCTGC
PflMI 230 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
984 CCACCCCATGG
SfaNI 88 GATGC GCATC(5/9)
38 GCATC
Tsp45I 469 GTCAC ^GT[GC]AC
919 GTGAC
Tsp509I 1034 AATT ^AATT
1259 AATT
Van91I 230 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
984 CCACCCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AfaI AluI AlwI BsaJI
BsiYI BslI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I FokI HincII HindII
HinfI MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NcoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI StyI TaqI
TfiI Tru9I TthHB8I XhoII