Restriction map for
pFN21AE5321
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspHI AspI AvaI AviII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlpI BmyI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DpnII
DraI DraII DraIII DrdI DsaI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NlaIV NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AciI 526 GCGG CCGC(-3/-1)
AflIII 546 ACATGT A^C[GA][CT]GT
AhdI 755 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 755 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 403 GGACC G^G[AT]CC
AvrII 27 CCTAGG C^CTAGG
BbsI 49 GTCTTC GAAGAC(2/6)
BlnI 27 CCTAGG C^CTAGG
BpuAI 49 GTCTTC GAAGAC(2/6)
BsmFI 244 GGGAC GGGAC(10/14)
BspDI 787 ATCGAT AT^CGAT
BspLU11I 546 ACATGT A^CATGT
BsrDI 660 CATTGC GCAATG(2/0)
BstBI 436 TTCGAA TT^CGAA
Cfr13I 403 GGACC G^G[ACGT]CC
ClaI 787 ATCGAT AT^CGAT
Eam1105I 755 GACCAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 327 GAAGAG CTCTTC(1/4)
EcoNI 27 CCTAGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 27 CCTAGG C^C[AT][AT]GG
Ksp632I 327 GAAGAG CTCTTC(1/4)
MaeII 16 ACGT A^CGT
MseI 784 TTAA T^TAA
NspV 436 TTCGAA TT^CGAA
Sau96I 403 GGACC G^G[ACGT]CC
SfaNI 160 GCATC GCATC(5/9)
SfuI 436 TTCGAA TT^CGAA
StuI 344 AGGCCT AGG^CCT
StyI 27 CCTAGG C^C[AT][AT]GG
Tru9I 784 TTAA T^TAA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 118 GAATTT [GA]^AATT[CT]
565 GAATTT
ApoI 118 GAATTT [GA]^AATT[CT]
565 GAATTT
Asp700I 498 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
795 GAAGAACTTC
BsgI 538 CTGCAC GTGCAG(16/14)
806 GTGCAG
BsrI 732 ACTGG ACTGG(1/-1)
840 ACTGG
BssKI 510 CCTGG ^CC[ACGT]GG
757 CCAGG
BstF5I 611 CATCC GGATG(2/0)
45 GGATG
BstNI 510 CCTGG CC^[AT]GG
757 CCAGG
EcoRII 510 CCTGG ^CC[AT]GG
757 CCAGG
FokI 611 CATCC GGATG(9/13)
45 GGATG
HaeIII 345 GGCC GG^CC
432 GGCC
HinfI 291 GACTC G^A[ACGT]TC
761 GAGTC
HphI 591 GGTGA GGTGA(8/7)
212 TCACC
MslI 541 CACACACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
543 CACACATGTG
MspA1I 535 CAGCTG C[AC]G^C[GT]G
632 CAGCTG
MvaI 510 CCTGG CC^[AT]GG
757 CCAGG
MwoI 424 GCTGTGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
625 GCTTGGACAGC
NspI 546 ACATGT [GA]CATG^[CT]
674 ACATGC
PleI 291 GACTC GAGTC(4/5)
761 GAGTC
PvuII 535 CAGCTG CAG^CTG
632 CAGCTG
ScrFI 510 CCTGG CC^[ACGT]GG
757 CCAGG
XmnI 498 GAATAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
795 GAAGAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AfaI AluI BbvI BfaI BsaJI
BsiYI BslI Cac8I DdeI Fnu4HI
ItaI MaeI MaeIII MboII MnlI
NlaIII RsaI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I