##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AfaI        
AflII       AgeI        AhdI        AlwI        AlwNI       
AocI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BsgI        BsiWI       
BsmI        Bsp1407I    BspDI       BspLU11I    BsrDI       
BsrGI       Bst1107I    BstAPI      BstBI       BstF5I      
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DdeI        DpnII       DraI        
DraIII      DrdI        Eam1105I    Eco81I      EcoNI       
EcoRI       EcoRV       EcoT22I     FbaI        FokI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        MamI        MboI        MfeI        
MflI        MluI        MseI        MslI        MunI        
NdeI        NdeII       NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RsaI        RsrII       SacI        SalI        Sau3AI      
SbfI        ScaI        SfcI        SfiI        SfuI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tth111I     XbaI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccI          667  GTCTAC               GT^[AC][GT]AC
AccIII       1104  TCCGGA               T^CCGGA
AflIII        218  ACGTGT               A^C[GA][CT]GT
Alw44I        873  GTGCAC               G^TGCAC
ApaLI         873  GTGCAC               G^TGCAC
AspHI         873  GTGCAC               G[AT]GC[AT]^C
BbsI         1073  GTCTTC               GAAGAC(2/6)
BpmI          202  CTCCAG               CTGGAG(16/14)
BpuAI        1073  GTCTTC               GAAGAC(2/6)
BsaAI         610  TACGTG               [CT]AC^GT[GA]
BsaI          883  GGTCTC               GGTCTC(1/5)
BsaWI        1104  TCCGGA               [AT]^CCGG[AT]
BseAI        1104  TCCGGA               T^CCGGA
BsiHKAI       873  GTGCAC               G[AT]GC[AT]^C
BsmBI         630  CGTCTC               CGTCTC(1/5)
BspEI        1104  TCCGGA               T^CCGGA
BspHI         518  TCATGA               T^CATGA
BspMI         164  ACCTGC               ACCTGC(4/8)
BsrBI        1027  CCGCTC               CCGCTC(-3/-3)
BstEII        444  GGTCACC              G^GT[ACGT]ACC
BstPI         444  GGTCACC              G^GT[ACGT]ACC
EcoO65I       444  GGTCACC              G^GT[ACGT]ACC
HgaI          547  GACGC                GACGC(5/10)
KspI          497  CCGCGG               CCGC^GG
MroI         1104  TCCGGA               T^CCGGA
NcoI           37  CCATGG               C^CATGG
NspI          602  ACATGC               [GA]CATG^[CT]
PflMI         182  CCATCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI          518  TCATGA               T^CATGA
SacII         497  CCGCGG               CCGC^GG
SapI          660  GCTCTTC              GCTCTTC(1/4)
SexAI         248  ACCTGGT              A^CC[AT]GGT
SmaI          969  CCCGGG               CCC^GGG
Tsp509I      1012  AATT                 ^AATT
Van91I        182  CCATCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           91  CCAGGGGTTCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         969  CCCGGG               C^CCGGG
XmaI          969  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

Aor51HI       452  AGCGCT               AGC^GCT
              560  AGCGCT              
BseRI         624  CTCCTC               GAGGAG(10/8)
              742  GAGGAG              
BssHII        838  GCGCGC               G^CGCGC
              942  GCGCGC              
BssSI          16  CACGAG               CACGAG(-5/-1)
              652  CTCGTG              
Eco47III      452  AGCGCT               AGC^GCT
              560  AGCGCT              
HphI          252  GGTGA                GGTGA(8/7)
              446  TCACC               
PvuII         467  CAGCTG               CAG^CTG
              700  CAGCTG              
SfaNI         657  GATGC                GCATC(5/9)
              371  GCATC               
SgrAI         275  CGCCGGCG             C[GA]^CCGG[CT]G
              752  CGCCGGCG            
TaqI          374  TCGA                 T^CGA
             1199  TCGA                
TthHB8I       374  TCGA                 T^CGA
             1199  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   83 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        ApaI        
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbvI        BcnI        BfaI        BglI        
BmyI        BsaHI       BsaJI       BsiEI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrFI       BsrI        BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DraII       
DsaI        EaeI        EagI        EarI        EclXI       
Eco52I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       ItaI        
KasI        Ksp632I     MaeI        MaeII       MaeIII      
MboII       MluNI       MnlI        MscI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NgoMIV      NlaIII      NlaIV       
PleI        Sau96I      ScrFI       SfoI        StyI        
TseI        Tsp45I      TspRI