Restriction map for
pFN21AE5234
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MroI MseI MslI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TfiI Tru9I Tsp45I Tsp509I TspRI
XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AocI 582 CCTGAGG CC^T[ACGT]AGG
AspI 187 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 196 TGGCCA TGG^CCA
BbsI 133 GTCTTC GAAGAC(2/6)
BglI 251 GCCGCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 420 GCTGAGC GC^T[ACGT]AGC
BmyI 3 GTGCCC G[AGT]GC[ACT]^C
BpmI 94 CTGGAG CTGGAG(16/14)
Bpu1102I 420 GCTGAGC GC^T[ACGT]AGC
BpuAI 133 GTCTTC GAAGAC(2/6)
BsmFI 88 GGGAC GGGAC(10/14)
Bsp1286I 3 GTGCCC G[AGT]GC[ACT]^C
BsrBI 145 GAGCGG CCGCTC(-3/-3)
BsrFI 290 GCCGGT [GA]^CCGG[CT]
BssSI 523 CTCGTG CACGAG(-5/-1)
BstBI 77 TTCGAA TT^CGAA
BstYI 440 AGATCC [GA]^GATC[CT]
Bsu36I 582 CCTGAGG CC^T[ACGT]AGG
CelII 420 GCTGAGC GC^T[ACGT]AGC
CfoI 418 GCGC GCG^C
Cfr10I 290 GCCGGT [GA]^CCGG[CT]
DraII 88 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 196 TGGCCA [CT]^GGCC[GA]
Eco81I 582 CCTGAGG CC^T[ACGT]AGG
EcoNI 522 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 88 GGGACCC [GA]G^G[ACGT]CC[CT]
FauI 44 CCCGC CCCGC(4/6)
HaeII 417 GGCGCT [GA]GCGC^[CT]
HhaI 418 GCGC GCG^C
HinP1I 418 GCGC G^CGC
HinfI 358 GACTC G^A[ACGT]TC
MaeII 269 ACGT A^CGT
MflI 440 AGATCC [GA]^GATC[CT]
MluNI 196 TGGCCA TGG^CCA
MscI 196 TGGCCA TGG^CCA
NspV 77 TTCGAA TT^CGAA
PaeR7I 83 CTCGAG C^TCGAG
PflFI 187 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 219 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 358 GACTC GAGTC(4/5)
PpuMI 88 GGGACCC [GA]G^G[AT]CC[CT]
SfuI 77 TTCGAA TT^CGAA
Tth111I 187 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 219 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 479 CCACCCTGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 83 CTCGAG C^TCGAG
XhoII 440 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 487 GTAC GT^AC
507 GTAC
AlwI 441 GATCC GGATC(4/5)
473 GATCC
AlwNI 589 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
619 CAGGGTCTG
AvaI 83 CTCGAG C^[CT]CG[GA]G
307 CCCGAG
AvaII 89 GGACC G^G[AT]CC
603 GGACC
BsaI 215 GAGACC GGTCTC(1/5)
68 GGTCTC
BseRI 151 CTCCTC GAGGAG(10/8)
97 GAGGAG
BsmAI 215 GAGAC GTCTC(1/5)
69 GTCTC
BsoBI 83 CTCGAG C^[CT]CG[GA]G
307 CCCGAG
BsrI 14 ACTGG ACTGG(1/-1)
489 ACTGG
DdeI 421 CTGAG C^T[ACGT]AG
583 CTGAG
DpnII 441 GATC ^GATC
473 GATC
MaeIII 11 GTAAC ^GT[ACGT]AC
176 GTAAC
MboI 441 GATC ^GATC
473 GATC
MboII 438 GAAGA GAAGA(8/7)
134 TCTTC
NdeII 441 GATC ^GATC
473 GATC
RsaI 487 GTAC GT^AC
507 GTAC
Sau3AI 441 GATC ^GATC
473 GATC
SmlI 63 CTCAAG C^T[CT][GA]AG
83 CTCGAG
StuI 201 AGGCCT AGG^CCT
311 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BcnI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cac8I Cfr13I EcoRII Fnu4HI FokI
HaeIII HapII HpaII ItaI MnlI
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfaNI TaqI
TseI TthHB8I