Restriction map for
pFN21AE4007
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# NO CUT ENZYMES ( 199 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BpmI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I Cfr10I
Cfr13I ClaI CpoI DpnII DraI
DraII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI HaeII HapII HgaI Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeII
MamI MboI MfeI MflI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI Sau3AI Sau96I
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AluI 458 AGCT AG^CT
AspHI 322 GTGCTC G[AT]GC[AT]^C
AviII 277 TGCGCA TGC^GCA
BbvI 167 GCTGC GCAGC(8/12)
BfaI 413 CTAG C^TAG
BlpI 150 GCTCAGC GC^T[ACGT]AGC
BmyI 322 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 150 GCTCAGC GC^T[ACGT]AGC
BsaJI 39 CCTGGG C^C[ACGT][ACGT]GG
BsiHKAI 322 GTGCTC G[AT]GC[AT]^C
BsiYI 39 CCTGGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 39 CCTGGGACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 322 GTGCTC G[AGT]GC[ACT]^C
BspMI 274 ACCTGC ACCTGC(4/8)
BsrBI 100 CCGCTC CCGCTC(-3/-3)
BsrDI 408 CATTGC GCAATG(2/0)
BstXI 7 CCATTCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 320 GCGTGC GC[ACGT]^[ACGT]GC
CelII 150 GCTCAGC GC^T[ACGT]AGC
CfoI 278 GCGC GCG^C
DraIII 200 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Fnu4HI 167 GCTGC GC^[ACGT]GC
FspI 277 TGCGCA TGC^GCA
HhaI 278 GCGC GCG^C
HinP1I 278 GCGC G^CGC
HinfI 444 GATTC G^A[ACGT]TC
ItaI 167 GCTGC GC^[ACGT]GC
MaeI 413 CTAG C^TAG
MseI 236 TTAA T^TAA
MwoI 380 GCTGTGTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 191 GGAACC GG[ACGT]^[ACGT]CC
TaqI 70 TCGA T^CGA
TfiI 444 GATTC G^A[AT]TC
Tru9I 236 TTAA T^TAA
TseI 167 GCTGC G^C[AT]GC
Tsp509I 260 AATT ^AATT
TspRI 13 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 70 TCGA T^CGA
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# TWO CUT ENZYMES ( 15 enzymes) #
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BbsI 292 GAAGAC GAAGAC(2/6)
142 GTCTTC
BpuAI 292 GAAGAC GAAGAC(2/6)
142 GTCTTC
BsmFI 42 GGGAC GGGAC(10/14)
416 GTCCC
BsrI 45 ACTGG ACTGG(1/-1)
12 CCAGT
BssKI 39 CCTGG ^CC[ACGT]GG
195 CCAGG
BstF5I 309 GGATG GGATG(2/0)
453 GGATG
BstNI 39 CCTGG CC^[AT]GG
195 CCAGG
DdeI 151 CTCAG C^T[ACGT]AG
420 CTCAG
EcoRII 39 CCTGG ^CC[AT]GG
195 CCAGG
FokI 309 GGATG GGATG(9/13)
453 GGATG
HaeIII 37 GGCC GG^CC
426 GGCC
MslI 8 CATTCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
108 CATCTATATG
MvaI 39 CCTGG CC^[AT]GG
195 CCAGG
NlaIII 147 CATG CATG^
393 CATG
ScrFI 39 CCTGG CC^[ACGT]GG
195 CCAGG
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# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
AciI HphI MaeIII MboII MnlI
SfaNI