Restriction map for
pFN21AE3419
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI BamHI BbeI BbrPI
BciVI BclI BfrI BglI BglII
BlpI Bpu1102I BsaBI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 619 GTCTAC GT^[AC][GT]AC
AcsI 1192 AAATTT [GA]^AATT[CT]
AcyI 1408 GACGCC G[GA]^CG[CT]C
AgeI 57 ACCGGT A^CCGGT
Aor51HI 564 AGCGCT AGC^GCT
ApoI 1192 AAATTT [GA]^AATT[CT]
AspHI 553 GTGCTC G[AT]GC[AT]^C
AspI 1269 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1124 TGCGCA TGC^GCA
AvrII 264 CCTAGG C^CTAGG
BanI 54 GGCACC G^G[CT][GA]CC
BanII 570 GGGCTC G[GA]GC[CT]^C
BbvCI 1361 CCTCAGC CCTCAGC(-5/-2)
BcgI 824 GCATCGTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 264 CCTAGG C^CTAGG
BsaHI 1408 GACGCC G[GA]^CG[CT]C
BsaWI 57 ACCGGT [AT]^CCGG[AT]
BsiEI 366 CGACCG CG[GA][CT]^CG
BsiHKAI 553 GTGCTC G[AT]GC[AT]^C
BsmFI 1381 GGGAC GGGAC(10/14)
DraI 922 TTTAAA TTT^AAA
DrdI 669 GACCGACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 564 AGCGCT AGC^GCT
EcoRV 944 GATATC GAT^ATC
FspI 1124 TGCGCA TGC^GCA
Hin1I 1408 GACGCC G[GA]^CG[CT]C
HincII 196 GTCAAC GT[CT]^[GA]AC
HindII 196 GTCAAC GT[CT]^[GA]AC
MseI 923 TTAA T^TAA
NspI 1059 ACATGC [GA]CATG^[CT]
PflFI 1269 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1092 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 57 ACCGGT A^CCGGT
PleI 682 GACTC GAGTC(4/5)
PpuMI 260 AGGTCCT [GA]G^G[AT]CC[CT]
SapI 555 GCTCTTC GCTCTTC(1/4)
SgrAI 56 CACCGGTG C[GA]^CCGG[CT]G
StuI 1004 AGGCCT AGG^CCT
Tru9I 923 TTAA T^TAA
Tsp509I 1193 AATT ^AATT
Tth111I 1269 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1092 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 333 CGCG CG^CG
616 CGCG
AfaI 609 GTAC GT^AC
1256 GTAC
Asp700I 17 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1191 GAAATTTTTC
AvaII 261 GGTCC G^G[AT]CC
478 GGACC
BbsI 289 GTCTTC GAAGAC(2/6)
1050 GTCTTC
BfaI 136 CTAG C^TAG
265 CTAG
BpuAI 289 GTCTTC GAAGAC(2/6)
1050 GTCTTC
BsaAI 550 TACGTG [CT]AC^GT[GA]
622 TACGTG
BspMI 167 ACCTGC ACCTGC(4/8)
581 ACCTGC
BsrI 272 ACTGG ACTGG(1/-1)
1029 CCAGT
BstUI 333 CGCG CG^CG
616 CGCG
BstYI 1160 GGATCT [GA]^GATC[CT]
1216 GGATCT
EarI 556 CTCTTC CTCTTC(1/4)
1200 CTCTTC
EcoNI 278 CCTTCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1151 CCTTCTACAGG
EcoT14I 71 CCAAGG C^C[AT][AT]GG
264 CCTAGG
HaeII 564 AGCGCT [GA]GCGC^[CT]
748 AGCGCC
Ksp632I 556 CTCTTC CTCTTC(1/4)
1200 CTCTTC
MaeI 136 CTAG C^TAG
265 CTAG
MaeII 551 ACGT A^CGT
623 ACGT
MflI 1160 GGATCT [GA]^GATC[CT]
1216 GGATCT
MvnI 333 CGCG CG^CG
616 CGCG
NaeI 51 GCCGGC GCC^GGC
501 GCCGGC
NgoMIV 51 GCCGGC G^CCGGC
501 GCCGGC
PvuII 954 CAGCTG CAG^CTG
1376 CAGCTG
RsaI 609 GTAC GT^AC
1256 GTAC
SfcI 1155 CTACAG C^T[GA][CT]AG
1385 CTACAG
SmaI 794 CCCGGG CCC^GGG
1338 CCCGGG
StyI 71 CCAAGG C^C[AT][AT]GG
264 CCTAGG
TaqI 257 TCGA T^CGA
365 TCGA
TfiI 1025 GAATC G^A[AT]TC
1351 GATTC
TthHB8I 257 TCGA T^CGA
365 TCGA
XcmI 1055 CCAAACATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1088 CCAGCCAGGCCGTGG
XhoII 1160 GGATCT [GA]^GATC[CT]
1216 GGATCT
XmaCI 794 CCCGGG C^CCGGG
1338 CCCGGG
XmaI 794 CCCGGG C^CCGGG
1338 CCCGGG
XmnI 17 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1191 GAAATTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI AvaI BalI
BbvI BcnI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrFI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SmlI TseI Tsp45I TspRI