Restriction map for
pFN21AE6473
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII BamHI BcgI BclI
BfrI BglII BsaBI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MluI MroI MslI NaeI NcoI
NdeI NgoMIV NheI NotI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tth111I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 340 GTAGAC GT^[AC][GT]AC
AccII 124 CGCG CG^CG
AcyI 168 GGCGCC G[GA]^CG[CT]C
AfaI 723 GTAC GT^AC
Asp700I 742 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1240 CCTAGG C^CTAGG
BanI 168 GGCGCC G^G[CT][GA]CC
BbeI 168 GGCGCC GGCGC^C
BbrPI 680 CACGTG CAC^GTG
BbsI 373 GTCTTC GAAGAC(2/6)
BciVI 1167 GGATAC GTATCC(6/5)
BfaI 1241 CTAG C^TAG
BlnI 1240 CCTAGG C^CTAGG
BpuAI 373 GTCTTC GAAGAC(2/6)
BsaAI 680 CACGTG [CT]AC^GT[GA]
BsaHI 168 GGCGCC G[GA]^CG[CT]C
BsaWI 929 ACCGGA [AT]^CCGG[AT]
BspMI 290 ACCTGC ACCTGC(4/8)
BsrBI 13 GAGCGG CCGCTC(-3/-3)
BssSI 1039 CTCGTG CACGAG(-5/-1)
BstAPI 285 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 124 CGCG CG^CG
DraIII 523 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 621 GAAGAG CTCTTC(1/4)
EcoNI 1158 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 168 GGCGCC [GA]GCGC^[CT]
Hin1I 168 GGCGCC G[GA]^CG[CT]C
HphI 129 GGTGA GGTGA(8/7)
KasI 168 GGCGCC G^GCGCC
Ksp632I 621 GAAGAG CTCTTC(1/4)
MaeI 1241 CTAG C^TAG
MfeI 1333 CAATTG C^AATTG
MseI 965 TTAA T^TAA
MunI 1333 CAATTG C^AATTG
MvnI 124 CGCG CG^CG
NarI 168 GGCGCC GG^CGCC
NruI 123 TCGCGA TCG^CGA
PaeR7I 637 CTCGAG C^TCGAG
PmaCI 680 CACGTG CAC^GTG
PmlI 680 CACGTG CAC^GTG
RsaI 723 GTAC GT^AC
SfoI 168 GGCGCC GGC^GCC
Tru9I 965 TTAA T^TAA
XcmI 1175 CCAAAGAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 637 CTCGAG C^TCGAG
XmnI 742 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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BalI 272 TGGCCA TGG^CCA
332 TGGCCA
BanII 544 GAGCTC G[GA]GC[CT]^C
1057 GAGCTC
BbvCI 436 GCTGAGG CCTCAGC(-5/-2)
478 GCTGAGG
BglI 805 GCCCGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
970 GCCTACCAGGC
BlpI 180 GCTGAGC GC^T[ACGT]AGC
546 GCTCAGC
BpmI 695 CTCCAG CTGGAG(16/14)
1138 CTGGAG
Bpu1102I 180 GCTGAGC GC^T[ACGT]AGC
546 GCTCAGC
CelII 180 GCTGAGC GC^T[ACGT]AGC
546 GCTCAGC
DsaI 275 CCACGG C^C[GA][CT]GG
527 CCGTGG
EaeI 272 TGGCCA [CT]^GGCC[GA]
332 TGGCCA
EcoT14I 686 CCAAGG C^C[AT][AT]GG
1240 CCTAGG
MaeII 681 ACGT A^CGT
707 ACGT
MluNI 272 TGGCCA TGG^CCA
332 TGGCCA
MscI 272 TGGCCA TGG^CCA
332 TGGCCA
PflMI 198 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
522 CCACCCCGTGG
SacI 544 GAGCTC GAGCT^C
1057 GAGCTC
SphI 814 GCATGC GCATG^C
894 GCATGC
StuI 236 AGGCCT AGG^CCT
887 AGGCCT
StyI 686 CCAAGG C^C[AT][AT]GG
1240 CCTAGG
Tsp509I 1148 AATT ^AATT
1334 AATT
Van91I 198 CCAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
522 CCACCCCGTGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaI AvaII BbvI BcnI BmyI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TseI
Tsp45I TspRI TthHB8I XhoII