Restriction map for
pFN21AE1711
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbsI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BpuAI BsaBI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstF5I
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AflIII 272 ACACGT A^C[GA][CT]GT
AlwI 153 GATCC GGATC(4/5)
AlwNI 43 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 576 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 121 GGCGCC G^G[CT][GA]CC
BanII 594 GGGCTC G[GA]GC[CT]^C
BbeI 121 GGCGCC GGCGC^C
BbrPI 193 CACGTG CAC^GTG
BcgI 341 GCAAGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 138 GCTGAGC GC^T[ACGT]AGC
BmyI 594 GGGCTC G[AGT]GC[ACT]^C
BpmI 400 CTGGAG CTGGAG(16/14)
Bpu1102I 138 GCTGAGC GC^T[ACGT]AGC
BsgI 196 GTGCAG GTGCAG(16/14)
BsmFI 113 GGGAC GGGAC(10/14)
Bsp1286I 594 GGGCTC G[AGT]GC[ACT]^C
BspMI 198 GCAGGT ACCTGC(4/8)
BssSI 436 CACGAG CACGAG(-5/-1)
BstAPI 371 GCAAAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 201 GGTCACC G^GT[ACGT]ACC
BstPI 201 GGTCACC G^GT[ACGT]ACC
BstYI 152 AGATCC [GA]^GATC[CT]
CelII 138 GCTGAGC GC^T[ACGT]AGC
DraIII 30 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 366 GAAGAG CTCTTC(1/4)
EcoO65I 201 GGTCACC G^GT[ACGT]ACC
EcoT14I 537 CCAAGG C^C[AT][AT]GG
HgaI 210 GCGTC GACGC(5/10)
KasI 121 GGCGCC G^GCGCC
Ksp632I 366 GAAGAG CTCTTC(1/4)
MaeIII 202 GTCAC ^GT[ACGT]AC
MboII 366 GAAGA GAAGA(8/7)
MflI 152 AGATCC [GA]^GATC[CT]
MseI 98 TTAA T^TAA
MslI 321 CATCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 121 GGCGCC GG^CGCC
PmaCI 193 CACGTG CAC^GTG
PmlI 193 CACGTG CAC^GTG
SapI 366 GAAGAGC GCTCTTC(1/4)
SfcI 171 CTACAG C^T[GA][CT]AG
SfoI 121 GGCGCC GGC^GCC
StyI 537 CCAAGG C^C[AT][AT]GG
TaqI 584 TCGA T^CGA
Tru9I 98 TTAA T^TAA
Tsp45I 202 GTCAC ^GT[GC]AC
Tsp509I 94 AATT ^AATT
TspRI 183 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 584 TCGA T^CGA
XcmI 251 CCAAGCTCATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 152 AGATCC [GA]^GATC[CT]
XmnI 576 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AcyI 121 GGCGCC G[GA]^CG[CT]C
209 GGCGTC
BsaAI 106 TACGTG [CT]AC^GT[GA]
193 CACGTG
BsaHI 121 GGCGCC G[GA]^CG[CT]C
209 GGCGTC
BsmAI 265 GAGAC GTCTC(1/5)
87 GTCTC
BsmBI 86 CGTCTC CGTCTC(1/5)
265 GAGACG
BsrI 102 CCAGT ACTGG(1/-1)
182 CCAGT
BstNI 117 CCAGG CC^[AT]GG
480 CCAGG
DpnII 153 GATC ^GATC
393 GATC
EcoRII 117 CCAGG ^CC[AT]GG
480 CCAGG
HaeII 121 GGCGCC [GA]GCGC^[CT]
527 AGCGCC
Hin1I 121 GGCGCC G[GA]^CG[CT]C
209 GGCGTC
HinfI 51 GATTC G^A[ACGT]TC
78 GAATC
HphI 81 TCACC GGTGA(8/7)
203 TCACC
MboI 153 GATC ^GATC
393 GATC
MvaI 117 CCAGG CC^[AT]GG
480 CCAGG
NdeII 153 GATC ^GATC
393 GATC
NlaIII 126 CATG CATG^
450 CATG
Sau3AI 153 GATC ^GATC
393 GATC
SfaNI 215 GCATC GCATC(5/9)
293 GCATC
TfiI 51 GATTC G^A[AT]TC
78 GAATC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BbvI BcnI BsaJI BsiYI BslI
BssKI BstUI Cac8I CfoI Cfr13I
DdeI FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MaeII
MnlI MspA1I MspI MvnI MwoI
NciI NlaIV PvuII RsaI Sau96I
ScrFI TseI