Restriction map for
pFN21AB6662
##################################
# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MflI MluI MluNI MroI
MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 1144 CGCG CG^CG
AhdI 1151 GACAGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1195 GTGCAC G^TGCAC
AlwI 12 GGATC GGATC(4/5)
ApaLI 1195 GTGCAC G^TGCAC
AspEI 1151 GACAGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1195 GTGCAC G[AT]GC[AT]^C
AvaI 831 CCCGGG C^[CT]CG[GA]G
BciVI 1174 GGATAC GTATCC(6/5)
BclI 444 TGATCA T^GATCA
BmyI 1195 GTGCAC G[AGT]GC[ACT]^C
BsgI 152 CTGCAC GTGCAG(16/14)
BsiEI 1128 CGATCG CG[GA][CT]^CG
BsiHKAI 1195 GTGCAC G[AT]GC[AT]^C
BsmAI 287 GTCTC GTCTC(1/5)
BsmI 510 GAATGC GAATGC(1/-1)
BsoBI 831 CCCGGG C^[CT]CG[GA]G
Bsp1286I 1195 GTGCAC G[AGT]GC[ACT]^C
BsrBI 1065 GAGCGG CCGCTC(-3/-3)
BstAPI 154 GCACTGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 192 CATCC GGATG(2/0)
BstUI 1144 CGCG CG^CG
Cac8I 73 GCTCGC GC[ACGT]^[ACGT]GC
CfoI 1143 GCGC GCG^C
EaeI 1104 CGGCCA [CT]^GGCC[GA]
Eam1105I 1151 GACAGGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 444 TGATCA T^GATCA
FokI 192 CATCC GGATG(9/13)
HhaI 1143 GCGC GCG^C
HinP1I 1143 GCGC G^CGC
MfeI 929 CAATTG C^AATTG
MslI 505 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1101 CAGCGG C[AC]G^C[GT]G
MunI 929 CAATTG C^AATTG
MvnI 1144 CGCG CG^CG
PstI 469 CTGCAG CTGCA^G
PvuI 1128 CGATCG CGAT^CG
ScaI 735 AGTACT AGT^ACT
SmaI 831 CCCGGG CCC^GGG
SmlI 792 CTTGAG C^T[CT][GA]AG
XmaCI 831 CCCGGG C^CCGGG
XmaI 831 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 1067 GCGG CCGC(-3/-1)
1103 GCGG
Asp700I 138 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
609 GAATACCTTC
BpmI 860 CTGGAG CTGGAG(16/14)
1334 CTGGAG
BseRI 172 CTCCTC GAGGAG(10/8)
646 GAGGAG
BsrDI 557 CATTGC GCAATG(2/0)
1296 GCAATG
BstXI 398 CCAACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
498 CCAGGGACATGG
EarI 313 GAAGAG CTCTTC(1/4)
366 GAAGAG
HphI 835 GGTGA GGTGA(8/7)
916 GGTGA
Ksp632I 313 GAAGAG CTCTTC(1/4)
366 GAAGAG
MaeIII 1124 GTGAC ^GT[ACGT]AC
1339 GTCAC
MwoI 154 GCACTGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
561 GCTTGATTTGC
NlaIV 826 GGACCC GG[ACGT]^[ACGT]CC
853 GGTCCC
PleI 953 GACTC GAGTC(4/5)
1337 GAGTC
SfaNI 70 GATGC GCATC(5/9)
1330 GATGC
SfcI 469 CTGCAG C^T[GA][CT]AG
1161 CTGTAG
Tsp45I 1124 GTGAC ^GT[GC]AC
1339 GTCAC
XmnI 138 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
609 GAATACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbvI BcnI BsaJI BsiYI BslI
BsmFI BsrI BssKI BssSI BstNI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII ItaI MboI MboII MnlI
MseI MspI MvaI NciI NdeII
NlaIII PpuMI RsaI Sau3AI Sau96I
ScrFI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I