Restriction map for
pFN21AE3220
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrGI
BssHII Bst1107I BstBI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT14I FbaI FseI FspI HaeII
Hin1I KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MunI NarI NciI NcoI
NdeI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 669 AAATTC [GA]^AATT[CT]
AlwI 1470 GATCC GGATC(4/5)
ApoI 669 AAATTC [GA]^AATT[CT]
Asp700I 561 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 595 GAAGAC GAAGAC(2/6)
BciVI 658 GTATCC GTATCC(6/5)
BpuAI 595 GAAGAC GAAGAC(2/6)
BsaJI 710 CCACGG C^C[ACGT][ACGT]GG
BseRI 953 GAGGAG GAGGAG(10/8)
BsiYI 399 CCAAACAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 399 CCAAACAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 342 GAATGC GAATGC(1/-1)
BsrFI 1228 GCCGGC [GA]^CCGG[CT]
BssSI 459 CACGAG CACGAG(-5/-1)
BstAPI 231 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1039 GGTAACC G^GT[ACGT]ACC
BstPI 1039 GGTAACC G^GT[ACGT]ACC
CfoI 205 GCGC GCG^C
Cfr10I 1228 GCCGGC [GA]^CCGG[CT]
DraIII 270 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 777 GACTATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 710 CCACGG C^C[GA][CT]GG
EaeI 44 TGGCCG [CT]^GGCC[GA]
EcoO65I 1039 GGTAACC G^GT[ACGT]ACC
EcoT22I 1343 ATGCAT ATGCA^T
FauI 959 GCGGG CCCGC(4/6)
HapII 1229 CCGG C^CGG
HgaI 202 GACGC GACGC(5/10)
HhaI 205 GCGC GCG^C
HinP1I 205 GCGC G^CGC
HindIII 286 AAGCTT A^AGCTT
HpaII 1229 CCGG C^CGG
MslI 1458 CACAACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 404 CAGCGG C[AC]G^C[GT]G
MspI 1229 CCGG C^CGG
NaeI 1228 GCCGGC GCC^GGC
NgoMIV 1228 GCCGGC G^CCGGC
NheI 585 GCTAGC G^CTAGC
NsiI 1343 ATGCAT ATGCA^T
NspI 1345 GCATGT [GA]CATG^[CT]
PleI 223 GACTC GAGTC(4/5)
PstI 120 CTGCAG CTGCA^G
SspI 1055 AATATT AAT^ATT
StuI 929 AGGCCT AGG^CCT
TfiI 1200 GATTC G^A[AT]TC
XmnI 561 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 204 CGCG CG^CG
416 CGCG
AflIII 179 ACGTGT A^C[GA][CT]GT
893 ACACGT
AlwNI 232 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
843 CAGAGACTG
BsaAI 178 TACGTG [CT]AC^GT[GA]
1105 TACGTG
BsgI 193 GTGCAG GTGCAG(16/14)
229 GTGCAG
BsrBI 159 CCGCTC CCGCTC(-3/-3)
208 CCGCTC
BssKI 303 CCAGG ^CC[ACGT]GG
1255 CCAGG
BstNI 303 CCAGG CC^[AT]GG
1255 CCAGG
BstUI 204 CGCG CG^CG
416 CGCG
Cac8I 585 GCTAGC GC[ACGT]^[ACGT]GC
1228 GCCGGC
DraII 115 GGGTCCT [GA]G^G[ACGT]CC[CT]
806 AGGACCC
EcoO109I 115 GGGTCCT [GA]G^G[ACGT]CC[CT]
806 AGGACCC
EcoRII 303 CCAGG ^CC[AT]GG
1255 CCAGG
HincII 423 GTTAAC GT[CT]^[GA]AC
897 GTTAAC
HindII 423 GTTAAC GT[CT]^[GA]AC
897 GTTAAC
HinfI 223 GACTC G^A[ACGT]TC
1200 GATTC
HpaI 423 GTTAAC GTT^AAC
897 GTTAAC
HphI 714 GGTGA GGTGA(8/7)
1203 TCACC
MvaI 303 CCAGG CC^[AT]GG
1255 CCAGG
MvnI 204 CGCG CG^CG
416 CGCG
NlaIV 115 GGGTCC GG[ACGT]^[ACGT]CC
807 GGACCC
PpuMI 115 GGGTCCT [GA]G^G[AT]CC[CT]
806 AGGACCC
SapI 69 GAAGAGC GCTCTTC(1/4)
161 GCTCTTC
ScrFI 303 CCAGG CC^[ACGT]GG
1255 CCAGG
TaqI 212 TCGA T^CGA
496 TCGA
Tsp45I 703 GTGAC ^GT[GC]AC
938 GTGAC
TthHB8I 212 TCGA T^CGA
496 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BfaI BpmI BsmAI BsmFI BsrI
BstF5I Cfr13I DdeI DpnII EarI
Fnu4HI FokI HaeIII ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MwoI NdeII NlaIII
RsaI Sau3AI Sau96I SfaNI SfcI
SmlI Tru9I TseI Tsp509I TspRI