Restriction map for
pFN21AE6676
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI Asp718I AspEI AspI AvaI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BseRI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspHI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
FbaI FseI FspI HaeII HincII
HindII HindIII HpaI HphI KasI
KpnI KspI MaeIII MamI MfeI
MunI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StyI SwaI TaqI Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccIII 383 TCCGGA T^CCGGA
AcyI 109 GGCGTC G[GA]^CG[CT]C
AseI 733 ATTAAT AT^TAAT
AsnI 733 ATTAAT AT^TAAT
Asp700I 180 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 65 GGATCC G^GATCC
BanII 248 GGGCTC G[GA]GC[CT]^C
BfaI 475 CTAG C^TAG
BglI 277 GCCGACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 496 CTGGAG CTGGAG(16/14)
BsaHI 109 GGCGTC G[GA]^CG[CT]C
BsaJI 59 CCTGGG C^C[ACGT][ACGT]GG
BseAI 383 TCCGGA T^CCGGA
BsgI 209 GTGCAG GTGCAG(16/14)
BsmAI 558 GTCTC GTCTC(1/5)
BsmI 147 GAATGC GAATGC(1/-1)
BspEI 383 TCCGGA T^CCGGA
BspMI 517 ACCTGC ACCTGC(4/8)
BsrBI 69 CCGCTC CCGCTC(-3/-3)
BstAPI 834 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 74 CCACGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 65 GGATCC [GA]^GATC[CT]
CfoI 286 GCGC GCG^C
DdeI 761 CTCAG C^T[ACGT]AG
DrdI 658 GACGGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 53 ATGCAT ATGCA^T
FauI 544 GCGGG CCCGC(4/6)
HgaI 110 GCGTC GACGC(5/10)
HhaI 286 GCGC GCG^C
Hin1I 109 GGCGTC G[GA]^CG[CT]C
HinP1I 286 GCGC G^CGC
MaeI 475 CTAG C^TAG
MaeII 487 ACGT A^CGT
MflI 65 GGATCC [GA]^GATC[CT]
MluI 205 ACGCGT A^CGCGT
MroI 383 TCCGGA T^CCGGA
MslI 75 CACGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 53 ATGCAT ATGCA^T
PshBI 733 ATTAAT AT^TAAT
SphI 273 GCATGC GCATG^C
StuI 240 AGGCCT AGG^CCT
XcmI 485 CCACGTTGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 65 GGATCC [GA]^GATC[CT]
XmnI 180 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 206 CGCG CG^CG
287 CGCG
AlwI 66 GATCC GGATC(4/5)
65 GGATC
AlwNI 580 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
835 CAGCTGCTG
AspHI 228 GAGCAC G[AT]GC[AT]^C
504 GAGCAC
BsaWI 383 TCCGGA [AT]^CCGG[AT]
777 TCCGGT
BsiHKAI 228 GAGCAC G[AT]GC[AT]^C
504 GAGCAC
BspLU11I 596 ACATGT A^CATGT
629 ACATGT
BstF5I 56 CATCC GGATG(2/0)
530 GGATG
BstUI 206 CGCG CG^CG
287 CGCG
DpnII 66 GATC ^GATC
378 GATC
DraII 191 GGGACCT [GA]G^G[ACGT]CC[CT]
260 GGGACCC
EarI 138 CTCTTC CTCTTC(1/4)
849 GAAGAG
EcoO109I 191 GGGACCT [GA]G^G[ACGT]CC[CT]
260 GGGACCC
FokI 56 CATCC GGATG(9/13)
530 GGATG
HapII 384 CCGG C^CGG
778 CCGG
HpaII 384 CCGG C^CGG
778 CCGG
Ksp632I 138 CTCTTC CTCTTC(1/4)
849 GAAGAG
MboI 66 GATC ^GATC
378 GATC
MseI 538 TTAA T^TAA
734 TTAA
MspA1I 130 CAGCTG C[AC]G^C[GT]G
835 CAGCTG
MspI 384 CCGG C^CGG
778 CCGG
MvnI 206 CGCG CG^CG
287 CGCG
NdeII 66 GATC ^GATC
378 GATC
PleI 759 GACTC GAGTC(4/5)
556 GAGTC
PpuMI 191 GGGACCT [GA]G^G[AT]CC[CT]
260 GGGACCC
PvuII 130 CAGCTG CAG^CTG
835 CAGCTG
Sau3AI 66 GATC ^GATC
378 GATC
SfaNI 531 GATGC GCATC(5/9)
55 GCATC
Tru9I 538 TTAA T^TAA
734 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AflIII AluI AvaII BalI
BbsI BbvI BmyI BpuAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I EaeI EcoRII
Fnu4HI HaeIII HinfI ItaI MboII
MluNI MnlI MscI MvaI MwoI
NlaIII NlaIV NspI Sau96I ScrFI
TfiI TseI TspRI