Restriction map for
pFN21AE1773
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DpnII DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MunI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Van91I XbaI XcmI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccII 475 CGCG CG^CG
AciI 474 CCGC CCGC(-3/-1)
AfaI 354 GTAC GT^AC
AlwNI 694 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 472 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 606 CTCGAG C^[CT]CG[GA]G
AvaII 32 GGACC G^G[AT]CC
BcgI 36 CGAGCTCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaJI 333 CCTGGG C^C[ACGT][ACGT]GG
BseRI 485 GAGGAG GAGGAG(10/8)
BsmFI 31 GGGAC GGGAC(10/14)
BsoBI 606 CTCGAG C^[CT]CG[GA]G
Bst1107I 194 GTATAC GTA^TAC
BstF5I 713 GGATG GGATG(2/0)
BstUI 475 CGCG CG^CG
BstZ17I 194 GTATAC GTA^TAC
EcoNI 11 CCTTCCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 713 GGATG GGATG(9/13)
HapII 270 CCGG C^CGG
HgaI 476 GCGTC GACGC(5/10)
HindIII 544 AAGCTT A^AGCTT
HpaII 270 CCGG C^CGG
MaeII 771 ACGT A^CGT
MspI 270 CCGG C^CGG
MvnI 475 CGCG CG^CG
MwoI 359 GCAGTAGTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 31 GGGACC GG[ACGT]^[ACGT]CC
PaeR7I 606 CTCGAG C^TCGAG
PflFI 472 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 357 CTGCAG CTGCA^G
RsaI 354 GTAC GT^AC
Tsp45I 416 GTCAC ^GT[GC]AC
TspRI 532 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 472 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 606 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 194 GTATAC GT^[AC][GT]AC
510 GTCTAC
AcsI 133 GAATTT [GA]^AATT[CT]
832 GAATTT
ApoI 133 GAATTT [GA]^AATT[CT]
832 GAATTT
BanII 37 GAGCTC G[GA]GC[CT]^C
609 GAGCTC
BbvI 693 GCAGC GCAGC(8/12)
746 GCAGC
BfaI 274 CTAG C^TAG
397 CTAG
BsiYI 11 CCTTCCATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
112 CCTGTGCATGG
BslI 11 CCTTCCATAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
112 CCTGTGCATGG
BsrI 464 ACTGG ACTGG(1/-1)
699 ACTGG
Cfr13I 32 GGACC G^G[ACGT]CC
142 GGCCC
DraI 777 TTTAAA TTT^AAA
792 TTTAAA
Fnu4HI 693 GCAGC GC^[ACGT]GC
746 GCAGC
HaeIII 142 GGCC GG^CC
217 GGCC
ItaI 693 GCAGC GC^[ACGT]GC
746 GCAGC
MaeI 274 CTAG C^TAG
397 CTAG
MseI 778 TTAA T^TAA
793 TTAA
PleI 25 GAGTC GAGTC(4/5)
508 GAGTC
SacI 37 GAGCTC GAGCT^C
609 GAGCTC
Sau96I 32 GGACC G^G[ACGT]CC
142 GGCCC
SfcI 357 CTGCAG C^T[GA][CT]AG
666 CTACAG
SmlI 286 CTTGAG C^T[CT][GA]AG
606 CTCGAG
TaqI 607 TCGA T^CGA
639 TCGA
TfiI 106 GATTC G^A[AT]TC
736 GATTC
Tru9I 778 TTAA T^TAA
793 TTAA
TseI 693 GCAGC G^C[AT]GC
746 GCAGC
TthHB8I 607 TCGA T^CGA
639 TCGA
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI AspHI BmyI BsiHKAI Bsp1286I
BssKI BstNI DdeI EcoRII HinfI
HphI MaeIII MboII MnlI MvaI
NlaIII ScrFI Tsp509I