Restriction map for
pFN21AE5447
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BcgI BciVI BcnI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstUI BstXI BstYI Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT14I EcoT22I FauI FseI
FspI HapII HgaI Hin1I HincII
HindII HpaI HpaII KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 397 GTATAC GT^[AC][GT]AC
AcsI 700 AAATTT [GA]^AATT[CT]
AlwI 939 GATCC GGATC(4/5)
AlwNI 544 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 700 AAATTT [GA]^AATT[CT]
AseI 712 ATTAAT AT^TAAT
AsnI 712 ATTAAT AT^TAAT
AvaII 861 GGTCC G^G[AT]CC
BanI 955 GGTGCC G^G[CT][GA]CC
BbsI 37 GAAGAC GAAGAC(2/6)
BbvCI 856 GCTGAGG CCTCAGC(-5/-2)
BclI 407 TGATCA T^GATCA
BfaI 745 CTAG C^TAG
BpuAI 37 GAAGAC GAAGAC(2/6)
BsaI 829 GAGACC GGTCTC(1/5)
BsaJI 761 CCCAGG C^C[ACGT][ACGT]GG
BspMI 952 GCAGGT ACCTGC(4/8)
BsrDI 582 CATTGC GCAATG(2/0)
Bst1107I 397 GTATAC GTA^TAC
BstEII 756 GGTGACC G^GT[ACGT]ACC
BstPI 756 GGTGACC G^GT[ACGT]ACC
BstZ17I 397 GTATAC GTA^TAC
CfoI 187 GCGC GCG^C
DraII 764 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 385 CTCTTC CTCTTC(1/4)
EcoO109I 764 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 756 GGTGACC G^GT[ACGT]ACC
EcoRV 748 GATATC GAT^ATC
FbaI 407 TGATCA T^GATCA
HaeII 186 AGCGCC [GA]GCGC^[CT]
HhaI 187 GCGC GCG^C
HinP1I 187 GCGC G^CGC
HindIII 112 AAGCTT A^AGCTT
Ksp632I 385 CTCTTC CTCTTC(1/4)
MaeI 745 CTAG C^TAG
MaeII 571 ACGT A^CGT
NlaIV 955 GGTGCC GG[ACGT]^[ACGT]CC
NspI 59 ACATGC [GA]CATG^[CT]
PflMI 864 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 712 ATTAAT AT^TAAT
SmlI 490 CTCAAG C^T[CT][GA]AG
StuI 149 AGGCCT AGG^CCT
Van91I 864 CCATCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AfaI 283 GTAC GT^AC
573 GTAC
BbvI 184 GCAGC GCAGC(8/12)
721 GCTGC
BsiYI 445 CCTATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
864 CCATCGGCTGG
BslI 445 CCTATCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
864 CCATCGGCTGG
BsmAI 829 GAGAC GTCTC(1/5)
970 GAGAC
BsmI 224 GAATGC GAATGC(1/-1)
456 GAATGC
BspHI 68 TCATGA T^CATGA
536 TCATGA
BsrI 825 ACTGG ACTGG(1/-1)
104 CCAGT
BssKI 549 CCTGG ^CC[ACGT]GG
762 CCAGG
BstNI 549 CCTGG CC^[AT]GG
762 CCAGG
Cfr13I 765 GGCCC G^G[ACGT]CC
861 GGTCC
DpnII 408 GATC ^GATC
939 GATC
EcoRII 549 CCTGG ^CC[AT]GG
762 CCAGG
Fnu4HI 184 GCAGC GC^[ACGT]GC
721 GCTGC
HaeIII 150 GGCC GG^CC
765 GGCC
HphI 756 GGTGA GGTGA(8/7)
274 TCACC
ItaI 184 GCAGC GC^[ACGT]GC
721 GCTGC
MaeIII 75 GTCAC ^GT[ACGT]AC
757 GTGAC
MboI 408 GATC ^GATC
939 GATC
MboII 37 GAAGA GAAGA(8/7)
386 TCTTC
MslI 77 CACACAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
627 CATATTTGTG
MvaI 549 CCTGG CC^[AT]GG
762 CCAGG
NdeII 408 GATC ^GATC
939 GATC
PleI 40 GACTC GAGTC(4/5)
670 GAGTC
RcaI 68 TCATGA T^CATGA
536 TCATGA
RsaI 283 GTAC GT^AC
573 GTAC
Sau3AI 408 GATC ^GATC
939 GATC
Sau96I 765 GGCCC G^G[ACGT]CC
861 GGTCC
ScrFI 549 CCTGG CC^[ACGT]GG
762 CCAGG
SfaNI 376 GATGC GCATC(5/9)
391 GATGC
TaqI 20 TCGA T^CGA
389 TCGA
TfiI 65 GATTC G^A[AT]TC
485 GAATC
TseI 184 GCAGC G^C[AT]GC
721 GCTGC
Tsp45I 75 GTCAC ^GT[GC]AC
757 GTGAC
TspRI 191 CACTG CA[GC]TG[ACGT][ACGT]^
450 CAGTG
TthHB8I 20 TCGA T^CGA
389 TCGA
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AluI BpmI BstF5I Cac8I DdeI
FokI HinfI MnlI MseI MwoI
NlaIII Tru9I Tsp509I