Restriction map for
pFN21AE5371
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BpuAI BsaAI BsaBI
BsaHI BsaI BseAI BsgI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CfoI ClaI CpoI DraII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeII MamI MluI
MluNI MroI MscI MspA1I MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I Van91I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 232 GTCTAC GT^[AC][GT]AC
AciI 366 GCGG CCGC(-3/-1)
AcsI 48 AAATTT [GA]^AATT[CT]
AgeI 643 ACCGGT A^CCGGT
AlwI 384 GATCC GGATC(4/5)
AlwNI 282 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 48 AAATTT [GA]^AATT[CT]
AseI 571 ATTAAT AT^TAAT
AsnI 571 ATTAAT AT^TAAT
AspHI 549 GAGCTC G[AT]GC[AT]^C
AvaI 276 CTCGAG C^[CT]CG[GA]G
BanII 549 GAGCTC G[GA]GC[CT]^C
BlpI 447 GCTTAGC GC^T[ACGT]AGC
BmyI 549 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 447 GCTTAGC GC^T[ACGT]AGC
BsaWI 643 ACCGGT [AT]^CCGG[AT]
BsiHKAI 549 GAGCTC G[AT]GC[AT]^C
BsiYI 644 CCGGTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 644 CCGGTTTCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 207 CGTCTC CGTCTC(1/5)
BsoBI 276 CTCGAG C^[CT]CG[GA]G
Bsp1286I 549 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 437 TGTACA T^GTACA
BsrFI 643 ACCGGT [GA]^CCGG[CT]
BsrGI 437 TGTACA T^GTACA
BstYI 383 AGATCC [GA]^GATC[CT]
Cac8I 399 GCTGGC GC[ACGT]^[ACGT]GC
CelII 447 GCTTAGC GC^T[ACGT]AGC
Cfr10I 643 ACCGGT [GA]^CCGG[CT]
DpnII 384 GATC ^GATC
DraI 45 TTTAAA TTT^AAA
DraIII 343 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
HaeIII 402 GGCC GG^CC
HapII 644 CCGG C^CGG
HpaII 644 CCGG C^CGG
MboI 384 GATC ^GATC
MfeI 90 CAATTG C^AATTG
MflI 383 AGATCC [GA]^GATC[CT]
MspI 644 CCGG C^CGG
MunI 90 CAATTG C^AATTG
MwoI 25 GCTATTATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 384 GATC ^GATC
NlaIV 778 GGGACC GG[ACGT]^[ACGT]CC
PaeR7I 276 CTCGAG C^TCGAG
PinAI 643 ACCGGT A^CCGGT
PleI 274 GACTC GAGTC(4/5)
PshBI 571 ATTAAT AT^TAAT
SacI 549 GAGCTC GAGCT^C
Sau3AI 384 GATC ^GATC
SexAI 160 ACCAGGT A^CC[AT]GGT
SspBI 437 TGTACA T^GTACA
TaqI 277 TCGA T^CGA
TthHB8I 277 TCGA T^CGA
XcmI 389 CCAGGCTGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 276 CTCGAG C^TCGAG
XhoII 383 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 315 GTAC GT^AC
438 GTAC
AvaII 741 GGTCC G^G[AT]CC
779 GGACC
BbvI 393 GCTGC GCAGC(8/12)
396 GCTGC
BpmI 559 CTCCAG CTGGAG(16/14)
818 CTCCAG
BseRI 123 CTCCTC GAGGAG(10/8)
609 GAGGAG
BsmFI 177 GGGAC GGGAC(10/14)
778 GGGAC
BsrI 561 CCAGT ACTGG(1/-1)
744 CCAGT
Cfr13I 741 GGTCC G^G[ACGT]CC
779 GGACC
EarI 126 CTCTTC CTCTTC(1/4)
210 CTCTTC
Fnu4HI 393 GCTGC GC^[ACGT]GC
396 GCTGC
ItaI 393 GCTGC GC^[ACGT]GC
396 GCTGC
Ksp632I 126 CTCTTC CTCTTC(1/4)
210 CTCTTC
MslI 570 CATTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
673 CATGTAGGTG
NlaIII 264 CATG CATG^
673 CATG
RsaI 315 GTAC GT^AC
438 GTAC
Sau96I 741 GGTCC G^G[ACGT]CC
779 GGACC
TseI 393 GCTGC G^C[AT]GC
396 GCTGC
Tsp509I 49 AATT ^AATT
91 AATT
TspRI 83 CAGTG CA[GC]TG[ACGT][ACGT]^
347 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BsaJI BsmAI BssKI BstF5I
BstNI DdeI EcoRII FokI HinfI
HphI MaeIII MboII MnlI MseI
MvaI ScrFI SfaNI SmlI TfiI
Tru9I Tsp45I