Restriction map for
pFN21AE5468
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KspI
MaeII MamI MluI MroI MslI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 563 GGTACC G^GTACC
AciI 434 CCGC CCGC(-3/-1)
Alw44I 407 GTGCAC G^TGCAC
AlwNI 637 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 407 GTGCAC G^TGCAC
Asp718I 563 GGTACC G^GTACC
AspHI 407 GTGCAC G[AT]GC[AT]^C
AvaII 934 GGACC G^G[AT]CC
BalI 834 TGGCCA TGG^CCA
BamHI 925 GGATCC G^GATCC
BanI 563 GGTACC G^G[CT][GA]CC
BclI 443 TGATCA T^GATCA
BfaI 380 CTAG C^TAG
BmyI 407 GTGCAC G[AGT]GC[ACT]^C
BpmI 660 CTCCAG CTGGAG(16/14)
BsaWI 896 TCCGGT [AT]^CCGG[AT]
BseRI 522 GAGGAG GAGGAG(10/8)
BsiHKAI 407 GTGCAC G[AT]GC[AT]^C
Bsp1286I 407 GTGCAC G[AGT]GC[ACT]^C
BspMI 566 ACCTGC ACCTGC(4/8)
BstAPI 636 GCAGATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 543 CCATGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 933 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 834 TGGCCA [CT]^GGCC[GA]
EcoO109I 933 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 929 CCAAGG C^C[AT][AT]GG
FbaI 443 TGATCA T^GATCA
HapII 897 CCGG C^CGG
HpaII 897 CCGG C^CGG
KpnI 563 GGTACC GGTAC^C
MaeI 380 CTAG C^TAG
MaeIII 499 GTGAC ^GT[ACGT]AC
MfeI 69 CAATTG C^AATTG
MluNI 834 TGGCCA TGG^CCA
MscI 834 TGGCCA TGG^CCA
MspA1I 99 CAGCTG C[AC]G^C[GT]G
MspI 897 CCGG C^CGG
MunI 69 CAATTG C^AATTG
PpuMI 933 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 99 CAGCTG CAG^CTG
StyI 929 CCAAGG C^C[AT][AT]GG
Tsp45I 499 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 59 GAATTC [GA]^AATT[CT]
208 GAATTC
AfaI 431 GTAC GT^AC
564 GTAC
ApoI 59 GAATTC [GA]^AATT[CT]
208 GAATTC
BbvI 98 GCAGC GCAGC(8/12)
642 GCTGC
BsrI 54 ACTGG ACTGG(1/-1)
335 CCAGT
BstF5I 705 CATCC GGATG(2/0)
395 GGATG
BstYI 666 AGATCC [GA]^GATC[CT]
925 GGATCC
Cac8I 294 GCTTGC GC[ACGT]^[ACGT]GC
832 GCTGGC
EarI 724 CTCTTC CTCTTC(1/4)
519 GAAGAG
EcoRI 59 GAATTC G^AATTC
208 GAATTC
Fnu4HI 98 GCAGC GC^[ACGT]GC
642 GCTGC
FokI 705 CATCC GGATG(9/13)
395 GGATG
ItaI 98 GCAGC GC^[ACGT]GC
642 GCTGC
Ksp632I 724 CTCTTC CTCTTC(1/4)
519 GAAGAG
MflI 666 AGATCC [GA]^GATC[CT]
925 GGATCC
MwoI 627 GCACACACGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
636 GCAGATGCTGC
NlaIII 226 CATG CATG^
544 CATG
RsaI 431 GTAC GT^AC
564 GTAC
SmlI 159 CTTGAG C^T[CT][GA]AG
364 CTCAAG
TseI 98 GCAGC G^C[AT]GC
642 GCTGC
XhoII 666 AGATCC [GA]^GATC[CT]
925 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI AlwI BsaJI BsiYI BslI
BsmFI BssKI BstNI Cfr13I DdeI
DpnII EcoRII HaeIII HinfI HphI
MboI MboII MnlI MseI MvaI
NdeII NlaIV PleI Sau3AI Sau96I
ScrFI SfaNI TfiI Tru9I Tsp509I
TspRI